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E4011

ExtrAvidin®−R-Phycoerythrin

buffered aqueous solution

Synonym(s):

R-Phycoerythrin

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Size/SKUAvailabilityPrice
1 mL
Please contact Customer Service for Availability
3 640,00 CZK

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00163600
Form:
buffered aqueous solution

3 640,00 CZK

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conjugate

phycoerythrin (R-PE) conjugate

Quality Segment

200

form

buffered aqueous solution

storage condition

protect from light

extent of labeling

1-4 mol PE per mol ExtrAvidin®

technique(s)

flow cytometry: suitable using human peripheral blood lymphocytes with biotinylated antibody, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 5 μg/mL using human tissues

storage temp.

2-8°C

General description

ExtrAvidin is a chemically modified protein purified from egg whites.
The extravidin-R-phycoerythrin protein can be conjugated with fluorescein-labelled arylacetamide, a κ opioid ligand and biotin-conjugated anti-fluorescein IgG to identify the subpopulations of thymocytes that express κ opioid receptor. This product has shown specificity for rabbit anti-avidin in immunoelectrophoresis analysis.
ExtrAvidin, a uniquely modified avidin reagent, combines the high specific activity and sensitivity of avidin with the low background staining of streptavidin. Avidin consists of four identical subunits, each containing a single biotin binding site.

Immunogen

Thymocytes present in C57BL mice.

Application

The ExtrAvidin-R-Phycoerythrin (PE) conjugate may be used in conjunction with biotinylated reagents in the avidin/biotin labeling system in flow cytometry.
ExtrAvidin®-R-Phycoerythrin has been used
  • In flow cytometric analysis to confirm membrane expression of ncamp-1 (cationic oligodeoxynucleotide binding protein).
  • In indirect immunofluorescence.
  • In Western blotting.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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ExtrAvidin®−R-Phycoerythrin buffered aqueous solution

Sigma-Aldrich

E4011

ExtrAvidin®−R-Phycoerythrin

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Sigma-Aldrich

E2513

ExtrAvidin®-Agarose, high binding

ExtrAvidin®−Peroxidase buffered aqueous solution

Sigma-Aldrich

E2886

ExtrAvidin®−Peroxidase

ExtrAvidin® –Alkaline Phosphatase buffered aqueous solution

Sigma-Aldrich

E2636

ExtrAvidin® –Alkaline Phosphatase

technique(s)

flow cytometry: suitable using human peripheral blood lymphocytes with biotinylated antibody, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 5 μg/mL using human tissues

technique(s)

-

technique(s)

direct ELISA: 1:2,000, dot blot: 1:4,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

technique(s)

dot blot: 1:300,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissues sections, indirect ELISA: 1:60,000

form

buffered aqueous solution

form

aqueous suspension

form

buffered aqueous solution

form

buffered aqueous solution

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage condition

protect from light

storage condition

-

storage condition

-

storage condition

-

conjugate

phycoerythrin (R-PE) conjugate

conjugate

-

conjugate

peroxidase conjugate

conjugate

alkaline phosphatase conjugate

extent of labeling

1-4 mol PE per mol ExtrAvidin®

extent of labeling

-

extent of labeling

-

extent of labeling

-

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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