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Merck

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E5160

Epidermal Growth Factor from mouse

≥90% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell cutlure

Sinônimo(s):

Mouse EGF, Mouse Epidermal Growth Factor

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Para você/SKUDisponibilidadePreço
100 μg
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R$ 1.601,00

Sobre este item

UNSPSC Code:
12352202
NACRES:
NA.77
Biological source:
mouse
Recombinant:
expressed in E. coli
Assay:
≥90% (SDS-PAGE)
Form:
lyophilized powder
Mol wt:
~6 kDa
Impurities:
≤1 EU/μg endotoxin (Protein)

R$ 1.601,00


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Nome do produto

Epidermal Growth Factor from mouse, EGF

biological source

mouse

Quality Segment

recombinant

expressed in E. coli

assay

≥90% (SDS-PAGE)

form

lyophilized powder

potency

0.05-1 ng/mL ED50/EC50

mol wt

~6 kDa

packaging

pkg of 100 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/μg endotoxin (Protein)

color

white

solubility

water: soluble 0.10 mL, clear, colorless

UniProt accession no.

storage temp.

−20°C

Gene Information

mouse ... Egf(13645)

General description

Epidermal Growth Factor (EGF) is a small mitogenic polypeptide (∼6kDa) present in many mammalian species and distributed throughout a wide number of tissues and body fluids. Four ErbB (HER) family receptor tyrosine kinases, including EGFR/ErbB1, ErbB2, ErbB3, and ErbB4 mediate responses to EGF family members. Human and mouse EGFs are very similar, but not identical in their physical and chemical properties. Of the 53 amino acid residues comprising each of the two polypeptides, 37 are common to both molecules, and three disulfide bonds are formed in the same relative positions.

Application

Epidermal Growth Factor from mouse has been used as a basal (N2B27) medium supplement for culturing differentiated mouse embryonic stem (E14) cells.

Biochem/physiol Actions

EGF (epidermal growth factor) is involved in cellular proliferation, differentiation, and survival. Moreover, it was found to affect various biological activities like angiogenesis, inhibition of gastric acid secretion, modulation of the synthesis of a number of hormones, synthesis and turn-over of proteins of the extracellular matrix, calcium release from bone tissue (thus promoting bone resorption), chemoattraction of fibroblasts and epithelial cells, and alone or in combination with other cytokines, mediation of wound healing processes. EGF is mitogenic for a large variety cell types, including fibroblasts, epithelial cells, endothelial cells, chondrocytes, and SV40-3T3 cells.

Physical form

The product is lyophilized from a 0.2 μm-filtered solution of phosphate buffered saline (PBS), pH 7.4.

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Este Item
E1257H9661V4512
assay

≥90% (SDS-PAGE)

assay

≥98% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥98% (SDS-PAGE)

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

recombinant

expressed in E. coli

recombinant

-

recombinant

expressed in NSO cells

recombinant

expressed in E. coli

biological source

mouse

biological source

mouse submaxillary glands

biological source

human

biological source

mouse

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

powder


Classe de armazenamento

11 - Combustible Solids

wgk

WGK 1



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