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EphB2/Fc Chimera from mouse

Name: EphB2/Fc Chimera from mouse
Brand: SIGMA
Price: 580 USD
Availability: InStock

>90% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

Synonym(s):

Cek5, Drt, ERK, Hek5, Nuk, Qek2, Sek3, Tyro5

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Size/SKU	Availability	Price
200 μg	Check Cart for Availability	$580.00

About This Item

UNSPSC Code:

51111800

NACRES:

NA.32

MDL number:

MFCD01867526

Assay:

>90% (SDS-PAGE)

Biological source:

mouse

Recombinant:

expressed in NSO cells

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Properties

biological source

mouse

Quality Segment

100

recombinant

expressed in NSO cells

assay

>90% (SDS-PAGE)

form

lyophilized powder

potency

0.04-2.5 ng/mL

mol wt

monomer calculated mol wt 85.3 kDa, 100-110 kDa by SDS-PAGE (reducing)

packaging

pkg of 200 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

ligand binding assay: suitable

impurities

endotoxin, tested

UniProt accession no.

P54763

storage temp.

−20°C

Gene Information

mouse ... Ephb2(13844)

Description

General description

EphB2 (Epherin receptor B2) is a receptor tyrosine kinase (RTK), which belongs to a 13 member family of ephrin receptors. This family is divided into two classes A and B. EphB2 belongs to EphB group, which contains five members. The ligands to this group of receptors are named ephrin-B1-B3. It is a bidirectional functioning membrane protein.

Application

EphB2/Fc Chimera from mouse has been used for the investigation of the role of ephrinB-EphB signaling in the induction and maintenance of hyperalgesia, hyperexcitability of neurons of DRG (dorsal root ganglion) and hyperexcitability and elevated synaptic plasticity of neurons of DH (dorsal horn) regions of brain.

Biochem/physiol Actions

EphB2 (Epherin receptor B2) is involved in the development and plasticity of glutamatergic synapses, and their interaction with NMDA receptor. It can also regulate the processing of acute inflammatory pain, and mechanical allodynia as a result of nerve crush. Activation of this receptor results in recruitment and activation of Src-protein kinases. These, in turn, phosphorylate and activate NMDA receptor. It regulates the function of platelets and the development of thrombus. It is also responsible for the retraction of clots, and modulates independent platelet function in a contact-independent manner. EphB2 signaling is also involved in the modulation of axonal sprouting, which is induced by lesions.

Member of the Eph receptor tyrosine kinase family shown to bind ephrin-B1, ephrin-B2, and ephrin-B3; involved in pattern formation and morphogenesis, and in arterial/venous demarcation of the developing vasculature.

Physical form

Lyophilized from a 0.2 μm filtered solution in 20 mM Tris, pH 8.0.

Analysis Note

The biological activity is measured by its ability to bind recombinant mouse ephrin-B2/Fc in an ELISA assay.

Other Notes

Extracellular domain of mouse EphB2 (amino acids 1-548) fused to the C-terminal 6X histidine-tagged Fc region of human IgG1 via a polypeptide linker.

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This Item	E0778
Sigma-Aldrich E9402 EphB2/Fc Chimera from mouse	Sigma-Aldrich E0778 Ephrin-B2/Fc Chimera from mouse
description >90% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder	description >90% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder
technique(s) ligand binding assay: suitable	technique(s) -
biological source mouse	biological source mouse
assay >90% (SDS-PAGE)	assay >90% (SDS-PAGE)
Gene Information mouse ... Ephb2(13844)	Gene Information mouse ... Efnb2(13642)
recombinant expressed in NSO cells	recombinant expressed in NSO cells
form lyophilized powder	form lyophilized powder

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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