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| Size/SKU | Availability | Price |
|---|---|---|
50 μg | Check Cart for Availability | $154.00 |
250 μg | Check Cart for Availability | $563.00 |
About This Item
recombinant
expressed in E. coli
Quality Segment
assay
≥95% (SDS-PAGE)
form
lyophilized powder
packaging
pkg of 1 kit (3 components)
application(s)
CRISPR
shipped in
ambient
storage temp.
−20°C
General description
Application
Biochem/physiol Actions
Features and Benefits
- Enhanced specificity compared to wild type Cas9
- Highly active
- Ready-to-inject/transfect
Packaging
pkg of 250 μg
Preparation Note
Other Notes
- one vial of eSpCas9 recombinant protein
- one vial containing 1 mL of 1× dilution buffer
- one vial containing 1 mL of nuclease-free water with glycerol
Check out our other MISSION® Cas9 Proteins at SigmaAldrich.com/CRISPRproteins
Legal Information
1 of 1
This Item | |||
|---|---|---|---|
| description from Streptococcus pyogenes with mutations conferring enhanced specificity, recombinant, expressed in E. coli, 1X NLS | description from Streptococcus pyogenes with mutations conferring enhanced specificity, fused with enhanced GFP, recombinant, expressed in E. coli, 3X NLS | description from Streptococcus pyogenes, recombinant, expressed in E. coli, 3X NLS | description from Streptococcus pyogenes, fused with enhanced GFP, recombinant, expressed in E. coli, 3X NLS |
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli |
| assay ≥95% (SDS-PAGE) | assay ≥90% (SDS-PAGE) | assay ≥95% (SDS-PAGE) | assay ≥90% (SDS-PAGE) |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| shipped in ambient | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| packaging pkg of 1 kit (3 components) | packaging pkg of 1 kit (3 components) | packaging pkg of 1 kit (4 components) | packaging pkg of 1 kit (3 components) |
Kit Components Only
- Enhanced specificity Cas9-NLS from Streptococcus pyogenes, expressed in Escherichia coli
- Dilution buffer for Cas9 proteins
- Reconstitution solution for Cas9 proteins
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
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