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F0522

Monoclonal Anti-CD3−FITC antibody produced in mouse

Name: Monoclonal Anti-CD3−FITC antibody produced in mouse
Brand: SIGMA
Price: 266.9 EUR

clone UCHT-1, purified immunoglobulin, buffered aqueous solution

Sinonimo/i:

Monoclonal Anti-CD3

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A voi/SKU	Disponibilità	Prezzo
50 test	Per informazioni sulla disponibilità, contatta il Servizio Clienti.	314,00 € 266,90 €

Informazioni su questo articolo

NACRES:

NA.44

UNSPSC Code:

12352203

Conjugate:

FITC conjugate

Clone:

UCHT-1, monoclonal

Application:

FACS

Citations:

266,90 €

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Proprietà

biological source

mouse

Quality Segment

200

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

UCHT-1, monoclonal

form

buffered aqueous solution

species reactivity

human

technique(s)

flow cytometry: 10 μL using 1 × 10⁶cells

isotype

IgG1

UniProt accession no.

P07766

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... CD3E(916)

Descrizione

General description

CD3e molecule, ε (CD3E) is a polypeptide encoded by the CD3E gene in humans. It resides on chromosome 11. The CD3ε cytoplasmic domain of the T cell receptor binds to the inner leaflet of the plasma membrane (PM) and is accessible during early stages of T cell activation. It is specific for T lymphocytes and is found in Purkinje cells of the cerebellum as well as in gastric parietal cells of humans and other species. Cytoplasmic cyCD3 expression can only be seen during the early phase of NK-cell differentiation.

The CD3 complex (16/20/29/22/25-28 kDa) consists of 5 polypeptide chains associated with each other and with the T cell receptor.

Immunogen

human thymocytes followed by Sezary T cells.

Application

Monoclonal Anti-CD3-FITC antibody produced in mouse is suitable for flow cytometry (at a dilution of 10μL using 1 × 106 cells). It was also used for ADCC Assay.

Biochem/physiol Actions

Recognizes the CD3 complex. The epitope recognized by clone no. UCHT-1 is expressed on the ε-chain of the CD3 antigen/T cell receptor complex. 3rd Workshop: code no. 126, 208 and 471
4th Workshop: paper no. T3.2

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Questo articolo	C7048	SAB4700555	SAB4700050
Sigma-Aldrich F0522 Monoclonal Anti-CD3−FITC antibody produced in mouse	Sigma-Aldrich C7048 Monoclonal Anti-CD3 antibody produced in mouse	Sigma-Aldrich SAB4700555 Monoclonal Anti-Cd3e-FITC antibody produced in hamster	Sigma-Aldrich SAB4700050 Monoclonal Anti-CD3 antibody produced in mouse
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin
clone UCHT-1, monoclonal	clone UCHT-1, monoclonal	clone 145-2C11, monoclonal	clone APA1/1, monoclonal
conjugate FITC conjugate	conjugate unconjugated	conjugate FITC conjugate	conjugate unconjugated
biological source mouse	biological source mouse	biological source hamster	biological source mouse
Gene Information human ... CD3E(916)	Gene Information human ... CD3E(916)	Gene Information mouse ... Cd3e(12501)	Gene Information human ... CD3(916)
species reactivity human	species reactivity human	species reactivity mouse	species reactivity human, mouse

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Classe di stoccaggio

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c