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F0552

Fas Ligand from mouse

>95% (SDS-PAGE), recombinant, expressed in mouse NSO cells, lyophilized powder

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50 μg
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$984.00

About This Item

UNSPSC Code:
12352200
NACRES:
NA.32
MDL number:
MFCD00285792

$984.00

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recombinant

expressed in mouse NSO cells

Quality Segment

100

assay

>95% (SDS-PAGE)

form

lyophilized powder

mol wt

monomer calculated mol wt ~18 kDa, 28-32 kDa by SDS-PAGE

impurities

endotoxin, tested

UniProt accession no.

P41047

storage temp.

−20°C

Gene Information

mouse ... Fasl(14103)

General description

FASLG (Fas ligand) acts as a ligand for Fas receptor, and is a major protein involved in programmed cell death, apoptosis. Soluble Fas (sFAS) is usually detected in plasma prior to apoptosis.[1]

Application

Fas Ligand (FASLG) from mouse has been used for-
  • the induction of apoptosis in PC12 cells and
  • the induction of migration in BV-2 murine microglial cells.[2][3]

Biochem/physiol Actions

FASLG (Fas ligand) and Fas receptor constitute the basic elements in apoptosis. Interaction of FASLG with Fas receptor leads to activation of caspase-8. This caspase in turn leads to activation of effector caspases such as caspase-3, -6 and -7. This cascade results in the hydrolysis of nuclear and cytoplasmic components. Expression of FASLG is induced by nuclear factor-κB (NFκB). NFκB/FASLG pathway facilitates the suppression of p,p′-DDT (dichlorodiphenoxytrichloroethane)-induced cell toxicity by vitamin C and E.[4] In CD4+ T cells, this protein is expressed on stimulus by T-cell receptor (TCR), both during normal and pathological conditions, such as alcohol exposure.[5]
Fas ligand, a protein belonging to the tumor necrosis factor (TNF) family of cytokines, induces apoptosis in cells expressing the cell membrane receptor Fas (CD95/Apo-1).
Protein belonging to the tumor necrosis factor (TNF) family of cytokines; induces apoptosis in cells expressing the cell membrane receptor Fas (CD95/Apo-1).

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.

Analysis Note

Measured by its ability to induce apoptosis in Jurkat cells.

Other Notes

Mouse Fas Ligand, N-terminal 6X histidine-tagged, encodes amino acid residues 132-279.

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S8689F8799PF033
Fas Ligand from mouse >95% (SDS-PAGE), recombinant, expressed in mouse NSO cells, lyophilized powder

Sigma-Aldrich

F0552

Fas Ligand from mouse

Fas Ligand, soluble human recombinant, expressed in HEK 293 cells, lyophilized powder

Sigma-Aldrich

S8689

Fas Ligand, soluble human

Fas/Fc Chimera from mouse >97% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

Sigma-Aldrich

F8799

Fas/Fc Chimera from mouse

Fas Ligand, Human, Recombinant Fas Ligand, Human, Recombinant, consists of amino acids 103-281 fused at the N-terminus to a tag-linker peptide. Binds to human, rat, and mouse Fas.

Sigma-Aldrich

PF033

Fas Ligand, Human, Recombinant

description

>95% (SDS-PAGE), recombinant, expressed in mouse NSO cells, lyophilized powder

description

recombinant, expressed in HEK 293 cells, lyophilized powder

description

>97% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

description

Fas Ligand, Human, Recombinant, consists of amino acids 103-281 fused at the N-terminus to a tag-linker peptide. Binds to human, rat, and mouse Fas.

recombinant

expressed in mouse NSO cells

recombinant

expressed in HEK 293 cells

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in HEK 293 cells

assay

>95% (SDS-PAGE)

assay

≥95% (SDS-GE)

assay

>97% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

100

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized

UniProt accession no.

P41047

UniProt accession no.

P48023

UniProt accession no.

P25446

UniProt accession no.

-

impurities

endotoxin, tested

impurities

-

impurities

endotoxin, tested

impurities

<0.1 EU/μg Endotoxin (EU/μg purified protein)

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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