The molecular weight of this enzyme has not been analyzed. The enzymatic assay states a solubility of 1 mg/ ml in water.
Immediately prior to pipetting into cuvette, prepare a solution at 1 mg / mL in cold purified water. Place on ice.
This solution is stable for a maximum of thirty minutes.
F0628
Ferredoxin-NADP+ Reductase from Spinacia oleracea (spinach)
lyophilized powder, ≥15 units/mg solid, secondary activity: ≥10 units/mg solid NADPH diaphorase
Synonym(s):
Ferridoxin-NADP+ oxidoreductase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥15 units/mg solid
Pricing and availability is not currently available.
form
lyophilized powder
Quality Level
specific activity
≥15 units/mg solid
foreign activity
NADH diaphorase activity ≤10 units/mg solid
storage temp.
−20°C
General description
Ferredoxin-NADP+ Reductase is a ferredoxin-soluble partner with a molar ratio to PSI of 0.9 and 3.
Application
Ferredoxin-NADP+ Reductase was used in in vitro ferredoxin-dependent desaturation of fatty acids in cyanobacterial thylakoid membranes.[1] It was also used to regulate glyceraldehyde-3-phosphate dehydrogenase.[2]
Ferredoxin-NADP+ Reductase from Spinacia oleracea (spinach) has been used to test the effect of potential protein partners on the activity of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH)−CP12 preparation. It has also been used in standard assay conditions for phycocyanobilin:ferredoxin oxidoreductase (PcyA) enzyme assay.
Biochem/physiol Actions
Ferredoxin-NADP+ reductase catalyzes the reversible conversion of reduced ferredoxin to oxidized ferredoxin during photosynthesis.[3] Ferredoxin-NADP(H) reductase constitutes a family of hydrophilic FAD-containing monomeric enzymes that deliver NADPH or low potential one-electron donors to redox-based metabolisms in plastids, mitochondria, and bacteria.[4]
Ferredoxin-NADP+ reductase catalyzes the reversible conversion of reduced ferredoxin to oxidized ferredoxin during photosynthesis[3]. Ferredoxin-NADP(H) reductase constitutes a family of hydrophilic FAD-containing monomeric enzymes that deliver NADPH or low potential one-electron donors to redox-based metabolisms in plastids, mitochondria, and bacteria.
Other Notes
One unit will reduce 1.0 millimole of cytochrome C per min at pH 7.5 at 25 °C in the presence of spinach ferredoxin and NADP.
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This Item | |||
|---|---|---|---|
| specific activity ≥15 units/mg solid | specific activity - | specific activity ≥40 units/mg protein (biuret) | specific activity - |
| form lyophilized powder | form lyophilized powder | form partially purified powder | form buffered aqueous solution |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −70°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| foreign activity NADH diaphorase activity ≤10 units/mg solid | foreign activity - | foreign activity - | foreign activity - |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Protocols
To measure ferredoxin NADP reductase activity, oxidized cytochrome C is used in a continuous spectrophotometric rate-determination assay at 550 nm.
[Laparocentesis in clinical traumatology].
V V Kuz'menko et al.
Khirurgiia, (6)(6), 50-54 (1986-06-01)
Manuel Twachtmann et al.
The Plant cell, 24(7), 2979-2991 (2012-07-19)
To adapt to different light intensities, photosynthetic organisms manipulate the flow of electrons through several alternative pathways at the thylakoid membrane. The enzyme ferredoxin:NADP(+) reductase (FNR) has the potential to regulate this electron partitioning because it is integral to most
Sara Baroni et al.
Biochemistry, 51(18), 3819-3826 (2012-04-24)
Plasmodium falciparum ferredoxin-NADP(+) reductase (FNR) is a FAD-containing enzyme that, in addition to be a promising target of novel antimalarial drugs, represents an excellent model of plant-type FNRs. The cofactor specificity of FNRs depends on differences in both k(cat) and
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Hi, how can I prepare a stock solution with the 1 U vial? It's not very clear for me in the specification sheet. I need a 1 uM final concentration of reductase for my assay, how do I prepare a stock solution of it?
1 answer-
Helpful?
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Hi there, could you please provide the specific methodology to extract this lyophilized powder from the vial? I didn't know how to extract this ferredoxin-NADP+ reductase from the vial
1 answer-
The vials contain less than 1mg solid so reconstituting the whole vial is recommended.
Helpful?
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