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F1269

Anti-Avidin–FITC antibody, Mouse monoclonal

clone WC19.10, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Avidin

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0.5 mL
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$411.00

About This Item

NACRES:
NA.43
UNSPSC Code:
12352203
Conjugate:
FITC conjugate
Clone:
WC19.10, monoclonal
Application:
IF, IHC (p)
Citations:
3

$411.00

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biological source

mouse

Quality Segment

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

WC19.10, monoclonal

form

buffered aqueous solution

storage condition

protect from light

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, indirect immunofluorescence: suitable

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Monoclonal Anti-Avidin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Avidin is a glycoprotein (68 kDa) composed of four identical polypeptide chains isolated from egg whites.
The antibody binds to the avidin molecule without interfering with the biotin-binding reaction. The product reacts specifically with egg white avidin, ExtrAvidin and their complexes formed with biotin. The antibody shows no cross-reaction with streptavidin.

Immunogen

Purified egg white avidin.

Application

Anti-Avidin-FITC antibody, Mouse monoclonal has been used in indirect immunohistology.

Biochem/physiol Actions

Avidin has a high binding affinity for the vitamin biotin. This strong interaction has been exploited in the design of immunoassays and immunohistologic staining techniques to serve as a basis for identifying antigen antibody interactions
FITC conjugated monoclonal anti-avidin may be used for single or double immunofluorescence labeling techniques and for modification of non-fluorescent to fluorescent reactions.

Physical form

Solution supplied in 0.01 M phosphate buffered saline, pH 8.0, containing 1% BSA and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-Avidin–FITC antibody, Mouse monoclonal clone WC19.10, purified from hybridoma cell culture

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Rabbit Anti-Goat IgG Antibody, F(ab′) 2, FITC conjugate Chemicon®, from rabbit

Sigma-Aldrich

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Rabbit Anti-Goat IgG Antibody, F(ab′) 2, FITC conjugate

description

clone WC19.10, purified from hybridoma cell culture

description

clone BN-34, purified from hybridoma cell culture

description

clone FL-D6, ascites fluid

description

Chemicon®, from rabbit

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

ascites fluid

antibody form

F(ab′)2 fragment of affinity isolated antibody

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

unconjugated

conjugate

FITC conjugate

clone

WC19.10, monoclonal

clone

BN-34, monoclonal

clone

FL-D6, monoclonal

clone

polyclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

rabbit

technique(s)

indirect immunofluorescence: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:80

technique(s)

indirect ELISA: 1:2,500 using FITC-conjugate, indirect immunofluorescence: suitable

technique(s)

immunofluorescence: suitable

form

buffered aqueous solution

form

buffered aqueous solution

form

-

form

-

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Storage Class

12 - Non Combustible Liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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