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Anti-β-Tubulin−FITC antibody, Mouse monoclonal

Name: Anti-β-Tubulin−FITC antibody, Mouse monoclonal
Brand: SIGMA
Price: 589 USD
Availability: InStock

clone TUB 2.1, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-β-Tubulin

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0.2 mL	Check Cart for Availability	$589.00

About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

eCl@ss:

32160702

Conjugate:

FITC conjugate

Clone:

TUB 2.1, monoclonal

Application:

IF (d)

Citations:

$589.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

FITC conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

TUB 2.1, monoclonal

form

buffered aqueous solution

mol wt

antigen 55 kDa

species reactivity

human, rat, frog, moth, mouse, plant, rabbit, chicken, bovine, wheat, sea urchin, hamster

storage condition

protect from light

technique(s)

direct immunofluorescence: 1:25 using cultured BHK cells

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

Description

General description

β-Tubulin belongs to the subfamily of tubulins. It has two β-sheets enclosed by α-helices. It also contains an N-terminal domain with the guanine nucleotide-binding region, an intermediate domain with taxol-binding site and a C-terminal domain, which has the binding surface for molecular motor proteins. Human β-tubulin consists of seven isoforms (βI (class I), βII (class II), βIII (class III), βIVa (class IVa), βIVb (class IVb), βV (class V), and βVI (class VI)). TUBB1 (tubulin beta class I) is located on human chromosome 6p21.33.(10)

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton. Tubulin is a heterodimer, which consists of α-tubulin and β-tubulin; both subunits have a molecular weight of 55 kDa and share considerable homology. The most studied tubulins have been isolated from vertebrate brains. The microtubules can be viewed in immunofluorescent microscopy, which enables the observation of the intracellular organization of proteins that are in the form of a supramolecular structure.

Immunogen

tubulin from rat brain.

Application

FITC-β tubulin antibody may be used for detection ajnd localization of β-tubulin by direct labeling in a variety of eukaryotic cells ranging from human embryonic kidney cells (HEK293T) to cells of azuki bean epicotyl. by immunohistochemical and immunocytochemical assays.

Monoclonal Anti-β-Tubulin-FITC antibody has been used:

in the evaluation of the status of the meiotic spindle and the arrangement of chromosomes
in immunofluorescence studies
in immunofluorescence microscopy
in spindle and chromosome imagery(6)

Biochem/physiol Actions

Mutation in β-Tubulin gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations. β-1 tubulin, also known as class VI or TUBB1, plays a vital role in platelet production and is considered to be a potential biomarker candidate for immune thrombocytopenia (ITP).

The antibody recognizes all five isoforms of β-tubulin (β1-β5). It reacts with the β-Lc and β-Sc fragments in the carboxy-terminal part of β−tubulin in immunoblotting. The antibody may be used to localize β−tubulin in cultured cells or tissue sections.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	C4585	T4026	T5201
Sigma-Aldrich F2043 Anti-β-Tubulin−FITC antibody, Mouse monoclonal	Sigma-Aldrich C4585 Anti-β-Tubulin−Cy3 antibody, Mouse monoclonal	Sigma-Aldrich T4026 Monoclonal Anti-β-Tubulin antibody produced in mouse	Sigma-Aldrich T5201 Anti-β-Tubulin antibody, Mouse monoclonal
description clone TUB 2.1, purified from hybridoma cell culture	description clone TUB 2.1, purified from hybridoma cell culture	description clone TUB 2.1, ascites fluid	description clone TUB 2.1, purified from hybridoma cell culture
species reactivity human, rat, frog, moth, mouse, plant, rabbit, chicken, bovine, wheat, sea urchin, hamster	species reactivity human, rat, frog, moth, mouse, plant, rabbit, chicken, bovine, wheat, sea urchin, hamster	species reactivity human, rat, frog, moth, mouse, plant, rabbit, chicken, bovine, wheat, sea urchin, hamster	species reactivity human, rat, frog, moth, mouse, plant, rabbit, chicken, bovine, wheat, sea urchin, hamster
antibody form purified from hybridoma cell culture	antibody form purified from hybridoma cell culture	antibody form ascites fluid	antibody form purified immunoglobulin
conjugate FITC conjugate	conjugate CY3 conjugate	conjugate unconjugated	conjugate unconjugated
biological source mouse	biological source mouse	biological source mouse	biological source mouse
clone TUB 2.1, monoclonal	clone TUB 2.1, monoclonal	clone TUB 2.1, monoclonal	clone TUB 2.1, monoclonal
Gene Information human ... TUBB(203068), TUBB1(81027), TUBB1(81027), TUBB1(81027), TUBB1(81027), TUBB2A(7280), TUBB2A(7280), TUBB2A(7280), TUBB2A(7280), TUBB2C(10383), TUBB2C(10383), TUBB2C(10383), TUBB2C(10383) mouse ... Tubb1(104068), Tubb1(104068), Tubb1(104068), Tubb1(104068), Tubb2a(22151), Tubb2a(22151), Tubb2a(22151), Tubb2a(22151), Tubb2c(227613), Tubb2c(227613), Tubb2c(227613), Tubb2c(227613), Tubb3(22152) rat ... Tubb2(29212), Tubb2(29212), Tubb2(29212), Tubb2(29212), Tubb2c(296554), Tubb2c(296554), Tubb2c(296554), Tubb2c(296554)	Gene Information human ... TUBB(203068), TUBB1(81027), TUBB1(81027), TUBB1(81027), TUBB1(81027), TUBB2A(7280), TUBB2A(7280), TUBB2A(7280), TUBB2A(7280), TUBB2C(10383), TUBB2C(10383), TUBB2C(10383), TUBB2C(10383) mouse ... Tubb1(104068), Tubb1(104068), Tubb1(104068), Tubb1(104068), Tubb2a(22151), Tubb2a(22151), Tubb2a(22151), Tubb2a(22151), Tubb2c(227613), Tubb2c(227613), Tubb2c(227613), Tubb2c(227613), Tubb3(22152) rat ... Tubb2(29212), Tubb2(29212), Tubb2(29212), Tubb2(29212), Tubb2c(296554), Tubb2c(296554), Tubb2c(296554), Tubb2c(296554)	Gene Information human ... TUBB(203068), TUBB1(81027), TUBB1(81027), TUBB1(81027), TUBB1(81027), TUBB2A(7280), TUBB2A(7280), TUBB2A(7280), TUBB2A(7280), TUBB2C(10383), TUBB2C(10383), TUBB2C(10383), TUBB2C(10383) mouse ... Tubb1(104068), Tubb1(104068), Tubb1(104068), Tubb1(104068), Tubb2a(22151), Tubb2a(22151), Tubb2a(22151), Tubb2a(22151), Tubb2c(227613), Tubb2c(227613), Tubb2c(227613), Tubb2c(227613) rat ... Tubb2(29212), Tubb2(29212), Tubb2(29212), Tubb2(29212), Tubb2c(296554), Tubb2c(296554), Tubb2c(296554), Tubb2c(296554)	Gene Information human ... TUBB(203068), TUBB1(81027), TUBB1(81027), TUBB1(81027), TUBB1(81027), TUBB2A(7280), TUBB2A(7280), TUBB2A(7280), TUBB2A(7280), TUBB2C(10383), TUBB2C(10383), TUBB2C(10383), TUBB2C(10383) mouse ... Tubb1(104068), Tubb1(104068), Tubb1(104068), Tubb1(104068), Tubb2a(22151), Tubb2a(22151), Tubb2a(22151), Tubb2a(22151), Tubb2c(227613), Tubb2c(227613), Tubb2c(227613), Tubb2c(227613), Tubb3(22152) rat ... Tubb2(29212), Tubb2(29212), Tubb2(29212), Tubb2(29212), Tubb2c(296554), Tubb2c(296554), Tubb2c(296554), Tubb2c(296554)

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Storage Class

12 - Non Combustible Liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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