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F2253

Riboflavin 5′-monophosphate sodium salt hydrate

synthetic, ≥70% (HPLC)

Synonym(s):

FMN-Na, Flavin mononucleotide, Riboflavin 5′-phosphate sodium salt

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10 mg
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RM 274.00
25 mg
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RM 480.00
100 mg
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RM 1,494.00

About This Item

Empirical Formula (Hill Notation):
C17H20N4NaO9P · xH2O
CAS Number:
130-40-5
Molecular Weight:
478.33 (anhydrous basis)
NACRES:
NA.51
PubChem Substance ID:
24894817
eCl@ss:
34058005
UNSPSC Code:
41106305
MDL number:
MFCD00150992
Beilstein/REAXYS Number:
4106529
Assay:
≥70% (HPLC)
Biological source:
synthetic
Form:
powder
Solubility:
water: 50 mg/mL
Storage temp.:
−20°C

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biological source

synthetic

Quality Level

200

assay

≥70% (HPLC)

form

powder

impurities

≤0.3% Riboflavin

solubility

water: 50 mg/mL

storage temp.

−20°C

SMILES string

O.[Na+].Cc1cc2N=C3C(=O)NC(=O)N=C3N(C[C@H](O)[C@H](O)[C@H](O)COP(O)([O-])=O)c2cc1C

InChI

1S/C17H21N4O9P.Na.H2O/c1-7-3-9-10(4-8(7)2)21(15-13(18-9)16(25)20-17(26)19-15)5-11(22)14(24)12(23)6-30-31(27,28)29;;/h3-4,11-12,14,22-24H,5-6H2,1-2H3,(H,20,25,26)(H2,27,28,29);;1H2/q;+1;/p-1/t11-,12+,14-;;/m0../s1

InChI key

BHRVCJBIICJWTH-APQIITSESA-M

Application

Riboflavin 5′-monophosphate sodium salt hydrate has been used:
  • as a component of assay buffer to determine the luminescence of L. lactis cells
  • as a component of the reaction mixture in nitric oxide synthase (NOS) enzymatic activity assay
  • in the high performance liquid chromatography (HPLC) analysis of Flavin mononucleotide (FMN) cyclase products
  • in luciferase assay with firefly luciferase[1]

Biochem/physiol Actions

Riboflavin 5′-monophosphate (FMN), a derivative of riboflavin, is used as a prosthetic group for one and two-electron transfer in oxidation/reduction reactions.[2] It acts as a cofactor for enzymes such as reduced nicotinamide adenine dinucleotide (NADH) dehydrogenase, nitric oxide synthase, and nitrilotriacetate monooxygenase. Riboflavin 5′-monophosphate is used as a substrate to study the specificity and kinetics of FMN phosphohydrolases[3] and to study molecular sensing-based gene regulation by riboswitches.[4]

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Riboflavin 5′-monophosphate sodium salt hydrate synthetic, ≥70% (HPLC)

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Riboflavin Sodium Phosphate Hydrate tested according to Ph. Eur.

Sigma-Aldrich

77623

Riboflavin Sodium Phosphate Hydrate

biological source

synthetic

biological source

non-animal source

biological source

-

biological source

synthetic

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

200

assay

≥70% (HPLC)

assay

70-79% (fluorimetric)

assay

≥70%

assay

-

form

powder

form

powder

form

powder

form

powder

solubility

water: 50 mg/mL

solubility

water: 50 mg/mL, orange

solubility

H2O: soluble 50 mg/mL, clear, orange

solubility

water: soluble 100 g/L at 20 °C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000528986
0000487306
0000487306
0000528986
0000229674

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Establishment of patterned thalamocortical connections does not require nitric oxide synthase
Finney E M and Shatz C J
The Journal of Neuroscience, 18(21), 8826-8838 (1998)
Luciferase detection during stationary phase in Lactococcus lactis
Bachmann H, et al.
Applied and Environmental Microbiology, 73(14), 4704-4706 (2007)
M Barile et al.
European journal of biochemistry, 249(3), 777-785 (1997-12-12)
In order to gain some insight into mitochondrial flavin biochemistry, rat liver mitochondria essentially free of lysosomal and microsomal contamination were prepared and their capability to metabolise externally added and endogenous FAD and FMN tested both spectroscopically and via HPLC.
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Global Trade Item Number

SKUGTIN
F2253-100MG04061833612842
F2253-10MG04061833612859
F2253-25MG04061833261637

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