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Anti-β-Actin–FITC antibody, Mouse monoclonal

Name: Anti-β-Actin–FITC antibody, Mouse monoclonal
Brand: SIGMA
Price: 624 USD
Availability: InStock

clone AC-15, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-β-Actin

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Size/SKU	Availability	Price
0.2 mL	Available to ship TODAYfromMILWAUKEE	$624.00

About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Conjugate:

FITC conjugate

Clone:

AC-15, monoclonal

Application:

IF (d)

Citations:

$624.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

AC-15, monoclonal

form

buffered aqueous solution

mol wt

antigen 42 kDa

species reactivity

sheep, carp, feline, chicken, rat, mouse, Hirudo medicinalis, rabbit, canine, pig, human, bovine, guinea pig

should not react with

Dictyostelium discoideum

technique(s)

direct immunofluorescence: 1:250 using cultured human and chicken fibroblasts

isotype

IgG1

UniProt accession no.

P60709

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ACTB(60)
mouse ... Actb(11461)
rat ... Actb(81822)

Description

General description

In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used.

Monoclonal Anti-b-Actin (mouse IgG1 isotype) is derived from the AC-15 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. ACTB (actin beta) gene codes for β-actin. It is a cytoskeletal housekeeping protein. It is present in the cytoplasm. This gene is located on human chromosome 7p22.1. Actin is a eukaryotic protein. It belongs to the major cytoskeletal protein family.

Immunogen

slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.

Application

Anti-β-Actin-FITC antibody, Mouse monoclonal has been used in:

western blotting
immunohistochemistry
flow cytometry
direct immunofluorescent staining
dual immuno-fluorescent staining

Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures.

Monoclonal mouse anti-actin was used as a loading control for western blot analysis of rat liver protein lysates.

Biochem/physiol Actions

β-Actin modulates cell growth, migration and the G-actin pool. Mutations in ACTB leads to pleiotropic developmental disorder.

Actins are majorly associated with cell motility. It is ubiquitously present in cytoplasm and involved in a myriad of cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. It is expressed in mammals and birds and at least six isoforms have been identified.

Actins is a eukaryotic protein. It belongs to the major cytoskeletal protein family and majorly associated with cell motility. It is ubiquitously present in cytoplasm and involved in a myriad of cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. It is expressed in mammals and birds and at least six isoforms have been identified.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	A5441	F3777	A3854
Sigma-Aldrich F3022 Anti-β-Actin–FITC antibody, Mouse monoclonal	Sigma-Aldrich A5441 Monoclonal Anti-β-Actin antibody produced in mouse	Sigma-Aldrich F3777 Anti-Actin, α-Smooth Muscle - FITC antibody, Mouse monoclonal	Sigma-Aldrich A3854 Anti-β-Actin−Peroxidase antibody, Mouse monoclonal
description clone AC-15, purified from hybridoma cell culture	description clone AC-15, ascites fluid	description clone 1A4, purified from hybridoma cell culture	description clone AC-15, purified from hybridoma cell culture
conjugate FITC conjugate	conjugate unconjugated	conjugate FITC conjugate	conjugate peroxidase conjugate
clone AC-15, monoclonal	clone AC-15, monoclonal	clone 1A4, monoclonal	clone AC-15, monoclonal
Gene Information human ... ACTB(60) mouse ... Actb(11461) rat ... Actb(81822)	Gene Information human ... ACTB(60) mouse ... Actb(11461) rat ... Actb(81822)	Gene Information human ... ACTA2(59) mouse ... Acta2(11475) rat ... Acta2(81633)	Gene Information human ... ACTB(60) mouse ... Actb(11461) rat ... Actb(81822)
antibody form purified immunoglobulin	antibody form ascites fluid	antibody form purified from hybridoma cell culture	antibody form purified immunoglobulin
biological source mouse	biological source mouse	biological source mouse	biological source mouse
species reactivity sheep, carp, feline, chicken, rat, mouse, Hirudo medicinalis, rabbit, canine, pig, human, bovine, guinea pig	species reactivity sheep, carp, feline, chicken, rat, mouse, Hirudo medicinalis, rabbit, canine, pig, human, bovine, guinea pig	species reactivity human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake	species reactivity sheep, carp, feline, chicken, rat, mouse, Hirudo medicinalis, rabbit, canine, pig, human, bovine, guinea pig

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c