Key Documents

Monoclonal Anti-Actin–FITC antibody produced in mouse

Name: Monoclonal Anti-Actin–FITC antibody produced in mouse
Brand: SIGMA
Price: 748 USD
Availability: InStock

clone AC-40, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Monoclonal Anti-Actin

Select a Size

Change View

Size/SKU	Availability	Price
0.2 mL	Estimated to ship TODAYfromMILWAUKEE	$748.00

About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Conjugate:

FITC conjugate

Clone:

AC-40, monoclonal

Application:

IF (d)

Citations:

$748.00

Estimated to ship TODAYDetails

Request a Custom Order

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

biological source

mouse

Quality Segment

200

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

AC-40, monoclonal

form

buffered aqueous solution

mol wt

antigen 42 kDa

species reactivity

human, Xenopus, hamster, mouse, guinea pig, rat, rabbit, snail, sheep, canine, carp, chicken, pig, bovine, viper, goat

technique(s)

direct immunofluorescence: 1:75 using cultured human or chicken fibroblasts

isotype

IgG2a

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

Description

General description

Monoclonal Anti-Actin (mouse IgG2a isotype) is derived from the AC-40 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. It has one N-terminal region which appears to be a major antigenic region and may be involved in the interaction of actin with other proteins such as myosin. Actin isoforms show 50-60% homology in their 18 N-terminal residues.

The antibody recognizes an epitope located on the C-terminal end of actin conserved in all actin isoforms. It specifically labels actin in a wide variety of tissues and species. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. Zinc-formalin, B5, ethanol, methacarn, Brunnel′s or Bouin′s solutions may also be used as fixatives.

Immunogen

synthetic actin C-terminal peptide, attached to a Multiple Antigen Peptide (MAP) backbone

Application

Actin–FITC antibody is suitable for direct immunofluorescence at a dilution of 1:75, using cultured human or chicken fibroblasts.

Monoclonal anti-actin antibody was used for western blot analysis of Cos-7 cell lysates to ensure equal protein loading.

Western blot analysis of MDCK cell lysates were performed using monoclonal anti-actin antibody as a primary antibody.

Biochem/physiol Actions

Actin, a conserved eukaryotic protein, is the major cytoskeletal component which actively participates in cell motility. It is associated with myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. In mammals and birds, actin is expressed as six different isoforms. It has one N-terminal region which appears to be a major antigenic region, and may be involved in the interaction of actin with other proteins such as myosin. Actin isoforms show 50-60% homology in their 18 N-terminal residues.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	A4700	A2103	F3777
Sigma-Aldrich F3046 Monoclonal Anti-Actin–FITC antibody produced in mouse	Sigma-Aldrich A4700 Monoclonal Anti-Actin antibody produced in mouse	Sigma-Aldrich A2103 Anti-Actin, N-terminal antibody produced in rabbit	Sigma-Aldrich F3777 Anti-Actin, α-Smooth Muscle - FITC antibody, Mouse monoclonal
description clone AC-40, purified immunoglobulin, buffered aqueous solution	description clone AC-40, ascites fluid	description ~0.5 mg/mL, affinity isolated antibody, buffered aqueous solution	description clone 1A4, purified from hybridoma cell culture
biological source mouse	biological source mouse	biological source rabbit	biological source mouse
conjugate FITC conjugate	conjugate unconjugated	conjugate unconjugated	conjugate FITC conjugate
antibody form purified immunoglobulin	antibody form ascites fluid	antibody form affinity isolated antibody	antibody form purified from hybridoma cell culture
species reactivity human, Xenopus, hamster, mouse, guinea pig, rat, rabbit, snail, sheep, canine, carp, chicken, pig, bovine, viper, goat	species reactivity human, Xenopus, hamster, mouse, guinea pig, rat, rabbit, snail, sheep, canine, carp, chicken, pig, bovine, viper, goat	species reactivity frog, rat, mouse, chicken, human	species reactivity human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake
Gene Information human ... ACTA1(58), ACTA1(58), ACTA1(58), ACTA2(59), ACTA2(59), ACTA2(59), ACTB(60), ACTC1(70), ACTC1(70), ACTC1(70), ACTG1(71), ACTG1(71), ACTG1(71), ACTG2(72), ACTG2(72), ACTG2(72) mouse ... Acta1(11459), Acta1(11459), Acta1(11459), Acta2(11475), Acta2(11475), Acta2(11475), Actb(11461), Actb(11461), Actb(11461), Actc1(11464), Actc1(11464), Actc1(11464), Actg1(11465), Actg1(11465), Actg1(11465), Actg2(11468), Actg2(11468), Actg2(11468) rat ... Acta1(29437), Acta1(29437), Acta1(29437), Acta2(81633), Acta2(81633), Acta2(81633), Actb(81822), Actb(81822), Actb(81822), Actc1(29275), Actc1(29275), Actc1(29275), Actg1(287876), Actg1(287876), Actg1(287876), Actg2(25365), Actg2(25365), Actg2(25365)	Gene Information human ... ACTA1(58), ACTA1(58), ACTA1(58), ACTA2(59), ACTA2(59), ACTA2(59), ACTB(60), ACTC1(70), ACTC1(70), ACTC1(70), ACTG1(71), ACTG1(71), ACTG1(71), ACTG2(72), ACTG2(72), ACTG2(72) mouse ... Acta1(11459), Acta1(11459), Acta1(11459), Acta2(11475), Acta2(11475), Acta2(11475), Actb(11461), Actb(11461), Actb(11461), Actc1(11464), Actc1(11464), Actc1(11464), Actg1(11465), Actg1(11465), Actg1(11465), Actg2(11468), Actg2(11468), Actg2(11468) rat ... Acta1(29437), Acta1(29437), Acta1(29437), Acta2(81633), Acta2(81633), Acta2(81633), Actb(81822), Actb(81822), Actb(81822), Actc1(29275), Actc1(29275), Actc1(29275), Actg1(287876), Actg1(287876), Actg1(287876), Actg2(25365), Actg2(25365), Actg2(25365)	Gene Information human ... ACTA1(58), ACTA2(59), ACTB(60), ACTC1(70), ACTG1(71), ACTG2(72) mouse ... Acta1(11459), Acta2(11475), Actb(11461), Actc1(11464), Actg1(11465), Actg2(11468) rat ... Acta1(29437), Acta2(81633), Actb(81822), Actc1(29275), Actg1(287876), Actg2(25365)	Gene Information human ... ACTA2(59) mouse ... Acta2(11475) rat ... Acta2(81633)
clone AC-40, monoclonal	clone AC-40, monoclonal	clone polyclonal	clone 1A4, monoclonal

Still not finding the right product?

Try our Product Selector Tool to narrow your options

Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c