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Monoclonal Anti-CD16−FITC antibody produced in mouse

Name: Monoclonal Anti-CD16−FITC antibody produced in mouse
Brand: SIGMA
Price: 536 USD
Availability: InStock

clone 3G8, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-CD16-II-Fluorescein isothiocyanate, Anti-CD16A-Fluorescein isothiocyanate, Anti-FCG3-Fluorescein isothiocyanate, Anti-FCGR3-Fluorescein isothiocyanate, Anti-FCGRIII-Fluorescein isothiocyanate, Anti-FCRIII-Fluorescein isothiocyanate

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Size/SKU	Availability	Price
50 test	Check Cart for Availability	$536.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.44

MDL number:

MFCD00675220

Conjugate:

FITC conjugate

Clone:

3G8, monoclonal

Application:

FACS

Citations:

$536.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

3G8, monoclonal

form

buffered aqueous solution

species reactivity

human

storage condition

protect from light

technique(s)

flow cytometry: 10 μL using 1 × 10⁶ cells

isotype

IgG1

UniProt accession no.

P08637

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... FCGR3A(2214), FCGR3B(2215)

Description

General description

CD16 antigen (also known as the low affinity receptor for complexed IgG, or Fcγ-RIII) expressed on natural killer (NK) cells, a macrophage sub-population and weakly on granulocytes. The human CD16 molecule exhibits two truncated Ig-like domains. In NK cells and macrophages, a trans-membrane form (50-80 kDa) with a 25 amino acid cytoplasmic tail is present. This form is non-covalently associated with other receptors (FcεRIγ or the TcRξ chain). The human CD16 molecule in granulocytes is a glycosyl-phosphatidyl-inositol (GPI)-linked form.

Human CD16 or FCγRIII is a 50-70kDa membrane glycoprotein expressed by neutrophils, eosinophils, cultured monocytes, natural killer (NK) cells, tissue macrophages subpopulation, and on a small subset of T cells that binds aggregated but not monomeric human IgG. It is also known as FcR3, the low affinity receptor for complexed IgG. It comprises of two truncated Ig-like cytoplasmic domains.

Monoclonal Anti-CD16 (mouse IgG1 isotype) is derived from the 3G8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized CD2F1 mouse.

Immunogen

viable human polymorphonuclear cells.

Application

Monoclonal Anti-CD16-FITC antibody is suitable for receptor blocking in flow cytometry analysis.

Monoclonal Anti-CD16-FITC antibody produced in mouse has been used in direct immunofluorescent staining.

Biochem/physiol Actions

Cluster of differentiation 16 (CD16) induces Ca²⁺ increase in neutrophils. It strongly labels neutrophils in tissue frozen sections. Liver kupffer cells are weakly stained. The antibody functionally blocks binding of soluble immune complexes to granulocytes and inhibits E-IgG rosettes.

Human CD16 plays a pivotal role in immune responses by linking the humoral immune system with cellular effector functions. CD16 plays an important role in natural killer (NK)-cell function and in peripheral blood mononuclear cells (PBMCs). In the FcyRIII-expressing lymphocytes the NK cell activity is highly influenced through FcyRIII. CD16 exists as a transmembrane form in NK cells, macrophages and cultured monocytes with a 25 amino acid cytoplasmic tail which further connects with other receptors. The transmembrane form binds complexed IgG and mediates phagocytosis and antibody-dependent cellular cytotoxicity (ADCC).

Recognizes the human CD16 antigen expressed on natural killer (NK) cells, granulocytes and a macrophage subpopulation. The antibody reacts with both NA1 and NA2 neutrophils and NK lymphocytes. It strongly labels neutrophils in tissue frozen sections. Liver Kupffer cells are weakly stained. The epitope recognized by the antibody seems to reside near the IgG binding site of FCγ-RIII. The antibody functionally blocks binding of soluble immune complexes to granulocytes and inhibits E-IgG rosettes. It induces Ca²⁺ increase in neutrophils.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	F9137	SAB5500046	SAB4700658
Sigma-Aldrich F3668 Monoclonal Anti-CD16−FITC antibody produced in mouse	Sigma-Aldrich F9137 Anti-Mouse IgG (whole molecule)−FITC antibody produced in rabbit	Sigma-Aldrich SAB5500046 Anti-CD16A antibody, Rabbit monoclonal	Sigma-Aldrich SAB4700658 Monoclonal Anti-HLA-DR-DP-FITC antibody produced in mouse
description clone 3G8, purified immunoglobulin, buffered aqueous solution	description -	description recombinant, expressed in proprietary host, clone SP189, affinity isolated antibody	description clone MEM-136, purified immunoglobulin, buffered aqueous solution
Gene Information human ... FCGR3A(2214), FCGR3B(2215)	Gene Information -	Gene Information human ... FCGR3A(2214)	Gene Information human ... HLA-DRA(3122)
clone 3G8, monoclonal	clone polyclonal	clone SP189, monoclonal	clone MEM-136, monoclonal
antibody form purified immunoglobulin	antibody form IgG fraction of antiserum	antibody form affinity isolated antibody	antibody form purified immunoglobulin
species reactivity human	species reactivity -	species reactivity human (tested)	species reactivity human
biological source mouse	biological source rabbit	biological source rabbit	biological source mouse
conjugate FITC conjugate	conjugate FITC conjugate	conjugate unconjugated	conjugate FITC conjugate

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

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