this works great for coating plates for cell culture
F4759
Bovine Fibronectin
from bovine plasma, powder, suitable for cell culture
Synonym(s):
Fibronectin
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| Size/SKU | Availability | Price |
|---|---|---|
1 mg | Check Cart for Availability | $256.00 |
2 mg | Check Cart for Availability | $419.00 |
5 mg | Check Cart for Availability | $832.00 |
About This Item
Biological source:
bovine plasma
Form:
powder
Technique(s):
cell culture | mammalian: suitable
Concentration:
≥1 mg protein/mL
Mol wt:
~450 kDa
Product Name
Fibronectin bovine plasma, powder, BioReagent, suitable for cell culture
biological source
bovine plasma
Quality Segment
sterility
sterile
product line
BioReagent
form
powder
mol wt
~450 kDa
packaging
pkg of 1 mg, pkg of 2 mg, pkg of 5 mg
concentration
≥1 mg protein/mL
technique(s)
cell culture | mammalian: suitable
surface coverage
1‑5 μg/cm2
solubility
H2O: 1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.)
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Gene Information
bovine ... FN1(2807940)
General description
Fibronectin is a glycoprotein with multiple domains. It comprises a N-terminal, central binding domain and a heparin-binding domain. Bovine plasma fibronectin has polypeptide chains with disulfide linkage and corresponds to a molecular weight of 220 and 215 kDa.
Fibronectins are adhesive glycoproteins and are found both as cell surface proteins and in plasma. Fibronectin (FN) is a multifunctional glycoprotein, the gene of which is localized to human chromosome 2q34-36. This gene spans 75kb and contains 50 exons. It exists as two major isoforms, one soluble and one insoluble form. The former is present in plasma, whereas the insoluble form resides in tissues and extracellular matrix (ECM) of cartilage. There are around 20 different forms of FN due to alternative splicing of a variable region and two type III exons called Extra Domains A and B.
Application
Fibronectin from bovine plasma is used for the following applications:
- Used for coating the glass coverslips for the culture of decidual stromal cells (DEC)
- Cell movement assays (culture plates were coated with fibronectin)
- Cell spreading and immunofluorescence
- Protein specificity
- Integration of sensor films into microfluidic chips
- Histological and Immunohistochemical (IHC) analyses
- Used in primary cell cultures from patients with lung carcinoma
Biochem/physiol Actions
Fibronectin is a regulator of protein scaffolding. It coordinates cellular processes like platelet functions, wound-healing, adhesion, migration and aggregation.Study shows that human dental pulp (HDP) cells rely on fibronectin (FN) for initial attachment and subsequent spreading. FN may exhibit mitogenic activity, promoting cell proliferation, and also play a role in regulating the differentiation process of pulp cells into odontoblasts. The presence of FN appears to influence the fate and development of HDP cells, specifically in their differentiation into odontoblasts, which are responsible for dentin formation in teeth.
Disclaimer
As supplied, this product should be stored at -20°C and will remain active for at least 2 years.
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This Item | |||
|---|---|---|---|
| description powder, BioReagent, suitable for cell culture | description solution, sterile-filtered, BioReagent, suitable for cell culture | description lyophilized powder, BioReagent, suitable for cell culture | description cell culture derived, ~0.5 mg/mL, sterile-filtered, BioReagent, suitable for cell culture |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| sterility sterile | sterility sterile-filtered | sterility sterile | sterility sterile-filtered |
| biological source bovine plasma | biological source bovine plasma | biological source human plasma | biological source human fibroblasts |
| concentration ≥1 mg protein/mL | concentration - | concentration >1 mg/mL protein | concentration ~0.5 mg/mL |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form powder | form solution | form lyophilized powder | form solution |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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