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Anti-Goat/Sheep IgG−FITC antibody, Mouse monoclonal

Name: Anti-Goat/Sheep IgG−FITC antibody, Mouse monoclonal
Brand: SIGMA

clone GT-34, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Goat/Sheep IgG

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About This Item

NACRES:

NA.46

UNSPSC Code:

12352203

Conjugate:

FITC conjugate

Clone:

GT-34, monoclonal

Application:

DIBA, IHC (p), PIFA

Citations:

Pricing and availability is not currently available.

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Properties

biological source

mouse

conjugate

FITC conjugate

antibody form

purified from hybridoma cell culture

antibody product type

secondary antibodies

clone

GT-34, monoclonal

form

buffered aqueous solution

species reactivity

sheep, goat, bovine

should not react with

rat, canine, mouse, rabbit, guinea pig, chicken, horse, pig, feline, human

storage condition

protect from light

technique(s)

dot immunobinding: 1:32, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20, particle immunofluorescence: 1:32

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Monoclonal Anti-Goat IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. IgG (immunoglobulin G) antibody subtype is a predominant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. IgG is found in four subclasses IgG1, IgG2, IgG3 and IgG4. IgG has a monomeric structure.

The antibody reacts with an epitope located on the heavy chain of goat IgG1 and IgG2.

Application

Biofile 39 Navya load

Biochem/physiol Actions

IgG (immunoglobulin G) antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. IgG antibody participates in complement fixation and opsonization. Goat antibodies against many analytes are widely used in research as primary antibodies in various assay techniques. Second antibodies are that used to detect goat antibodies may lack specificity for goat immunoglobulins, and in many instances will also recognize other immunoglobulins that appear in the preparation being tested. This results in the need for extensive adsorption of the second antibody. Therefore, using a monoclonal antibody to goat IgG that is devoid of a binding capacity to other species can serve as a useful tool in many applications.

Monoclonal Anti-Goat IgG, FITC Conjugate is immunospecific for goat IgG. The antibody does not react with human, mouse, rat, rabbit, guinea pig, horse, or chicken IgGs.

Physical form

Solution in 0.01 M sodium phosphate buffer, pH 7.4, containing 1% inactivated bovine serum albumin and 15 mM sodium azide

Preparation Note

For continuous use, store at 2-8 °C for a maximum of one month. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	A8062	SAB4200804	F9890
Sigma-Aldrich F4891 Anti-Goat/Sheep IgG−FITC antibody, Mouse monoclonal	Sigma-Aldrich A8062 Anti-Goat/Sheep IgG−Alkaline Phosphatase antibody, Mouse monoclonal	Sigma-Aldrich SAB4200804 Anti-Goat/sheep IgG antibody, Mouse monoclonal	Sigma-Aldrich F9890 Anti-Human IgG4−FITC antibody, Mouse monoclonal
technique(s) dot immunobinding: 1:32, particle immunofluorescence: 1:32, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20	technique(s) direct ELISA: 1:50,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40, western blot (chemiluminescent): 1:150,000 using total cell extract of chicken fibroblasts (10-20 μg per well)	technique(s) indirect ELISA: 0.25-0.5 μg/mL using 5μg/ml Goat IgG for coating.	technique(s) dot immunobinding: 1:64 using using a 4-8 μg dot of human IgG4
species reactivity sheep, goat, bovine	species reactivity sheep, goat, bovine	species reactivity goat, sheep	species reactivity -
conjugate FITC conjugate	conjugate alkaline phosphatase conjugate	conjugate -	conjugate FITC conjugate
antibody form purified from hybridoma cell culture	antibody form purified from hybridoma cell culture	antibody form purified from hybridoma cell culture	antibody form purified from hybridoma cell culture
biological source mouse	biological source mouse	biological source mouse	biological source mouse
isotype IgG1	isotype IgG1	isotype IgG1	isotype IgG1

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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