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F5636

Monoclonal Anti-FITC antibody produced in mouse

clone FL-D6, ascites fluid

Synonym(s):

Monoclonal Anti-FITC

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Size/SKUAvailabilityPrice
0.2 mL
Please contact Customer Service for Availability
$762.00
0.5 mL
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$1,540.00

About This Item

NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
FL-D6, monoclonal
Application:
ELISA (i), IF
Citations:
7

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biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

FL-D6, monoclonal

contains

15 mM sodium azide

technique(s)

indirect ELISA: 1:2,500 using FITC-conjugate, indirect immunofluorescence: suitable

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques because the conjugation of FITC to proteins is relatively easy and generally does not destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Antibodies to FITC are used to identify FITC labeled proteins and as models to study the mechanism of antibody response to a well defined hapten.

Immunogen

FITC-BSA conjugate

Application

Mouse monoclonal clone FL-D6 anti-FITC antibody may be used for the detection of FITC and as a universal indicator reagent for bridging FITC with other immunochemical reagents. It may be used in ELISA, competitive ELISA[1] and immunofluorescent techniques. A FITC Anti-FITC system has been used in the amplification of signal in immunofluorescent detection and as a means of separating bound from free tracer by affinity chromatography. The antibody can also be used to isolate cells that have an FITC-labeled ligand on their surface.

Biochem/physiol Actions

Mouse monoclonal clone FL-D6 anti-FITC antibody will react with either free or conjugated FITC. The antibody does not react with bound or free TRITC (tetramethylrhodamine isothiocyanate).
The antibody reacts with both free and conjugated fluorescein isothiocyanate (FITC).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Monoclonal Anti-FITC antibody produced in mouse clone FL-D6, ascites fluid

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Monoclonal Anti-FITC−Alkaline Phosphatase antibody produced in mouse clone FL-D6, purified immunoglobulin, buffered aqueous solution

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Anti-FITC antibody, Mouse monoclonal clone FL-D6, purified from hybridoma cell culture

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Monoclonal Anti-FITC−Biotin antibody produced in mouse clone FL-D6, purified immunoglobulin, buffered aqueous solution

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B0287

Monoclonal Anti-FITC−Biotin antibody produced in mouse

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

clone

FL-D6, monoclonal

clone

FL-D6, monoclonal

clone

FL-D6, monoclonal

clone

FL-D6, monoclonal

conjugate

unconjugated

conjugate

alkaline phosphatase conjugate

conjugate

-

conjugate

biotin conjugate

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

technique(s)

indirect ELISA: 1:2,500 using FITC-conjugate, indirect immunofluorescence: suitable

technique(s)

dot blot: 1:80,000 using human IgG/FITC monoclonal anti-human IgG, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20 using FITC conjugate, indirect ELISA: 1:30,000

technique(s)

immunoblotting: 1.25-2.5 μg/mL using ExtrAvidin-FITC, immunohistochemistry: suitable, indirect ELISA: 0.15-0.3 μg/mL using 5 μg/ml ExtrAvidin-FITC

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 using human tonsil

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

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Storage Class

10 - Combustible liquids

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flash_point_f

Not applicable

flash_point_c

Not applicable

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