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Anti-Filamin antibody,Mouse monoclonal

Name: Anti-Filamin antibody,Mouse monoclonal
Brand: SIGMA
Price: 604 EUR

clone PM6/317, purified from hybridoma cell culture

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Size/SKU	Availability	Price
200 μL	Please contact Customer Service for Availability	€604.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00162292

Conjugate:

unconjugated

Clone:

PM6/317, monoclonal

Application:

IF, WB CL

Citations:

€604.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

PM6/317, monoclonal

form

buffered aqueous solution

mol wt

antigen ~190 kDa

species reactivity

human, monkey, hamster, bovine, canine

concentration

~1.0 mg/mL

technique(s)

indirect immunofluorescence: suitable, western blot (chemiluminescent): 0.5-1.0 μg/mL using whole extract of human Jurkat or HEK-293Tcells

isotype

IgG1

UniProt accession no.

P21333

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... FLNA(2316)

Description

General description

Filamin, also known as actin-binding protein, is a large flexible V-shaped actin cross-linking cytoplasmic protein present in vertebrates of both muscle and non-muscle cells. It has actin-binding domains at the ends of each arm. It is a polypeptide with molecular weight of approximately 250 kDa and forms dimers in solution. It has a large flexible actin cross-linking protein present in vertebrates in both the muscle and non-muscle cells. The name filamins refers to its filamentous colocalization with actin stress fibers. It has an actin binding domain and a homodimerization domain. Native form comprises of two subunits with one actin-binding site.

Monoclonal Anti-Filamin (mouse IgG1 isotype) is derived from the hybridoma PM6/317 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human platelet membranes.

Immunogen

human platelet membranes

Application

Monoclonal Anti-Filamin antibody is suitable for indirect immunofluorescence and western blot (chemiluminescent) at a concentration of 0.5-1.0μg/mL using whole extract of human Jurkat or HEK-293Tcells.

Monoclonal Anti-Filamin antibody produced in mouse has been used in:

immunoblotting
immunofluorescent staining[1][2]
immunoprecipitation[2]

Biochem/physiol Actions

Filamin has been primarily identified from smooth muscle but it has also found in macrophages, neutrophils, platelets and other cell types. It is associated in regulation of the cytoskeletal network by cross-linking actin and linking cell membrane to the cytoskeleton. It is also linked in regulating intracellular signaling pathways, responsible for skeletal development. It binds to the ring zinc finger domain of tumor necrosis factor (TNF) and receptor-associated factor 2 (TRAF2). Filamin cross-links with actin and promotes its aggregation and gelation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	PLA0168	SAB5700454	SAB4200760
Sigma-Aldrich F6682 Anti-Filamin antibody,Mouse monoclonal	Sigma-Aldrich PLA0168 Rabbit anti-Filamin A Antibody, Affinity Purified	Sigma-Aldrich SAB5700454 Anti-Phospho-FLNA-S2152 antibody produced in rabbit	Sigma-Aldrich SAB4200760 Anti-Fibronectin antibody, Mouse monoclonal
antibody form purified from hybridoma cell culture	antibody form affinity purified immunoglobulin	antibody form affinity isolated antibody	antibody form purified from hybridoma cell culture
clone PM6/317, monoclonal	clone polyclonal	clone polyclonal	clone FN-15, monoclonal
conjugate unconjugated	conjugate unconjugated	conjugate -	conjugate -
Gene Information human ... FLNA(2316)	Gene Information human ... FLNA(2316)	Gene Information human ... FLNA(2316)	Gene Information human ... FN1(2335)
species reactivity human, monkey, hamster, bovine, canine	species reactivity human, mouse	species reactivity rat, mouse, human	species reactivity human, mouse
biological source mouse	biological source rabbit	biological source rabbit	biological source mouse

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c