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F6682

Anti-Filamin antibody,Mouse monoclonal

clone PM6/317, purified from hybridoma cell culture

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200 μL

Available to ship TODAYfromMILWAUKEE

$664.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD00162292
Conjugate:
unconjugated
Clone:
PM6/317, monoclonal
Application:
IF, WB CL
Citations:
13

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biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

PM6/317, monoclonal

form

buffered aqueous solution

mol wt

antigen ~190 kDa

species reactivity

human, monkey, hamster, bovine, canine

concentration

~1.0 mg/mL

technique(s)

indirect immunofluorescence: suitable, western blot (chemiluminescent): 0.5-1.0 μg/mL using whole extract of human Jurkat or HEK-293Tcells

isotype

IgG1

UniProt accession no.

P21333

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... FLNA(2316)

General description

Filamin, also known as actin-binding protein, is a large flexible V-shaped actin cross-linking cytoplasmic protein present in vertebrates of both muscle and non-muscle cells. It has actin-binding domains at the ends of each arm. It is a polypeptide with molecular weight of approximately 250 kDa and forms dimers in solution. It has a large flexible actin cross-linking protein present in vertebrates in both the muscle and non-muscle cells. The name filamins refers to its filamentous colocalization with actin stress fibers. It has an actin binding domain and a homodimerization domain. Native form comprises of two subunits with one actin-binding site.
Monoclonal Anti-Filamin (mouse IgG1 isotype) is derived from the hybridoma PM6/317 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human platelet membranes.

Immunogen

human platelet membranes

Application

Monoclonal Anti-Filamin antibody is suitable for indirect immunofluorescence and western blot (chemiluminescent) at a concentration of 0.5-1.0μg/mL using whole extract of human Jurkat or HEK-293Tcells.
Monoclonal Anti-Filamin antibody produced in mouse has been used in:
  • immunoblotting
  • immunofluorescent staining[1][2]
  • immunoprecipitation[2]

Biochem/physiol Actions

Filamin has been primarily identified from smooth muscle but it has also found in macrophages, neutrophils, platelets and other cell types. It is associated in regulation of the cytoskeletal network by cross-linking actin and linking cell membrane to the cytoskeleton. It is also linked in regulating intracellular signaling pathways, responsible for skeletal development. It binds to the ring zinc finger domain of tumor necrosis factor (TNF) and receptor-associated factor 2 (TRAF2). Filamin cross-links with actin and promotes its aggregation and gelation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-Filamin antibody,Mouse monoclonal clone PM6/317, purified from hybridoma cell culture

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Gene Information

human ... FLNA(2316)

Gene Information

human ... FLNA(2316)

Gene Information

human ... FN1(2335)

Gene Information

bovine ... ACTG1(404122)
chicken ... ACTG1(415296)
human ... ACTG1(71)
mouse ... Actg1(11465)

species reactivity

human, monkey, hamster, bovine, canine

species reactivity

human

species reactivity

human, mouse

species reactivity

mouse, chicken, canine, bovine, human, hamster

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

clone

PM6/317, monoclonal

clone

3B6-1D10, monoclonal

clone

FN-15, monoclonal

clone

2-2.1.14.17, monoclonal

conjugate

unconjugated

conjugate

unconjugated

conjugate

-

conjugate

unconjugated

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000424122
0000424122
0000406480
0000406480
0000203186

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Related Content

Data Sheet - F6682

Instructions

Structure and Organization of Actin Filaments
Cooper GM et al.
Cell (2000)
Sayaka Yamamoto et al.
Molecular biology of the cell, 27(3), 491-499 (2015-12-04)
At the initial step of carcinogenesis, transformation occurs in single cells within epithelia, where the newly emerging transformed cells are surrounded by normal epithelial cells. A recent study revealed that normal epithelial cells have an ability to sense and actively
Takanori Chiba et al.
Scientific reports, 6, 28383-28383 (2016-06-22)
Cell competition is a cell-cell interaction by which a cell compares its fitness to that of neighboring cells. The cell with the relatively lower fitness level is the "loser" and actively eliminated, while the cell with the relatively higher fitness
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Global Trade Item Number

SKUGTIN
F6682-200UL04061837720420

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