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Anti-Fibroblast Growth Factor Receptor-2, Extracellular antibody produced in rabbit

Name: Anti-Fibroblast Growth Factor Receptor-2, Extracellular antibody produced in rabbit
Brand: SIGMA
Price: 624.75 USD
Availability: InStock

affinity isolated antibody, buffered aqueous solution

Enhanced Validation

Synonym(s):

Anti-FGFR-2

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Size/SKU	Availability	Price
0.2 mL	Check Cart for Availability	$735.00 $624.75

About This Item

UNSPSC Code:

51111800

NACRES:

NA.41

MDL number:

MFCD02096345

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IHC (p), WB

Citations:

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 115-125 kDa (doublet)

species reactivity

human

packaging

antibody small pack of 25 μL

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using trypsin-digested, human and animal tissue sections, western blot: 1:2,000 using extract of FGFR-2 transiently transfected cells

UniProt accession no.

P21802

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... FGFR2(2263)

Description

General description

Fibroblast growth factors (FGFs) belongs to the large tyrosine kinase family of growth factor receptors (17-38kDa). The FGF family consists of at least seventeen members designated FGF-1 through FGF-17 that bind to high affinity FGF receptors (FGFRs). Soluble, secreted or possibly cleaved forms of FGFR-1 and FGFR-2 have also been found in body fluids. They are glycosylated 110-150 kDa proteins that are constructed of an extracellular ligand binding region with either two, or typically three, immunoglobulin (Ig)-like domains and an eight amino acid "acidic box", a transmembrane region and a cytoplasmic split tyrosine kinase domain that is activated following ligand binding and receptor dimerization. The ligand binding site of all FGFRs is confined to the extracellular Ig-like domains 2 and 3.

Immunogen

synthetic peptide corresponding to amino acids 362-374 of the extracellular region of human FGFR-2 (Bek).

Application

Anti-Fibroblast Growth Factor Receptor-2, Extracellular antibody produced in rabbit has been used in immunoprecipitation and immunoblotting.

Biochem/physiol Actions

Fibroblast growth factors (FGFs) are potent physiological regulators normal development, wound healing and repair, angiogenesis, a variety of neurotrophic activities, in hematopoiesis as well as in tissue remodeling and maintenance. FGFRs exhibit overlapping recognition and redundant specificity. Signal transduction by FGFRs requires dimerization or oligomerization and autophosphorylation of the receptors through their tyrosine kinase domain. Subsequent association with cytoplasmic signaling molecules leads to DNA synthesis or differentiation. The signaling and biological responses elicited by distinct FGFRs substantially differ. FGFR-2 is highly expressed in developing human tissues including the brain (preferentially in glial cells), choroid plexus, skin, lung, kidney and bone. It may be found in several anchorage-dependent cells, such as normal and malignant breast cancer cells.

The antibody detects human FGFR-2 (bek) in extracts of transfected cells.

The antibody specifically reacts with human FGFR-2; no reaction with FGFR-1 or FGFR-3 is detected.

Physical form

Solution in 0.01 M phosphate buffered saline,
pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Preparation Note

Affinity isolated on a peptide-agarose column.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	F0300	F3922	F5421
Sigma-Aldrich F6796 Anti-Fibroblast Growth Factor Receptor-2, Extracellular antibody produced in rabbit	Sigma-Aldrich F0300 Anti-Fibroblast Growth Factor Receptor-2, Cytoplasmic antibody produced in rabbit	Sigma-Aldrich F3922 Anti-Fibroblast Growth Factor Receptor-3, Extracellular antibody produced in rabbit	Sigma-Aldrich F5421 Anti-Fibroblast Growth Factor Receptor-1 antibody produced in rabbit
description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution	description ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
technique(s) western blot: 1:2,000 using extract of FGFR-2 transiently transfected cells, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using trypsin-digested, human and animal tissue sections	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2-4 μg/mL using trypsin-digested, human tissue sections, western blot: 0.25-0.5 μg/mL using extracts/lysates of FGFR-2 transfected cells	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using protease-digested, human and animal tissue sections, western blot: 1:500 using extract of FGFR-3 transfected cells	technique(s) immunoprecipitation (IP): suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using trypsin-digested human and animal tissue sections, western blot: 1:400 using an extract of FGFR-1 transfected cells
species reactivity human	species reactivity human	species reactivity human	species reactivity human

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Storage Class

10 - Combustible liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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