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Monoclonal Anti-CD31 (PECAM-1)−FITC antibody produced in mouse

Name: Monoclonal Anti-CD31 (PECAM-1)−FITC antibody produced in mouse
Brand: SIGMA
Price: 653 USD
Availability: InStock

clone WM-59, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Monoclonal Anti-CD31, Anti-PECAM-1

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100 test	Check Cart for Availability	$653.00

About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Conjugate:

FITC conjugate

Clone:

WM-59, monoclonal

Application:

FACS

Citations:

$653.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

WM-59, monoclonal

form

buffered aqueous solution

mol wt

antigen 130-140 kDa

species reactivity

human

storage condition

protect from light

technique(s)

flow cytometry: 10 μL using 1 × 10⁶ cells

isotype

IgG1

UniProt accession no.

P16284

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... PECAM1(5175)

Description

General description

CD31 is a single chain integral membrane glycoprotein member of the immunoglobulin superfamily of cell adhesion molecules with molecular mass of 130-140kDa. It has six Ig-like loops in the extracellular domain, a trans-membrane domain including a relatively long cytoplasmic domain. It is expressed abundantly on platelets, myeloid cells, B lymphocytes, certain T-lymphocyte cell subsets, bone marrow precursor cells, NK cells and localized to the intracellular junctions in monolayers of cultured endothelial cells.

Human CD31 (PECAM-1) is expressed on platelets, endothelial cells, myeloid cells, B lymphocytes, and certain T lymphocyte subsets. CD31 (PECAM-1) functions in homophilic and heterophilic cell-cell adhesion and cell signaling activities.

Monoclonal Anti-CD31 (PECAM-1) is derived from the WM-59 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Immunogen

human cell line RC-2A, originally derived from myeloid leukemia cells.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)

Monoclonal Anti-CD31 (PECAM-1)-FITC antibody has been used in

DAB based-immunohistochemistry
direct immunofluorescent staining
fluorescence-activated cell sorting (FACS)
flow cytometry

Monoclonal Anti-CD31 (PECAM-1)-FITC antibody is suitable for immunostaining of HEPC-CB2 and HEPC-CB.2 cell Lines (hematopoietic cells) and flow cytometry to evaluate the percentage of contaminating endothelia cells in characterization and decidualization of endometrial stromal cells.

Biochem/physiol Actions

CD31 is also known as PECAM-1 (Platelet endothelial cell adhesion molecule-1) binds to glycosaminoglycans (GAG). Endothelial CD31 is phosphorylated on tyrosine and serine residues. CD31 is involved in some of the interactive events taking place during cardiovascular development inflammation, thrombosis, wound healing and angiogenesis.

CD31 performs in homophilic and heterophilic cell-cell adhesion and cell signalling activities. It plays a major role in the transmigration of monocytes, neutrophils and NK cells between the endothelial cell junctions into the subendothelial matrix.

Recognizes the CD31 (PECAM-1, endoCAM, gpIIa, hec7), human cell surface integral membrane glycoprotein. The antibody increases the rate of homotypic aggregation induced in U937 cells by TGF-β1. Its reactivity with platelets is enhanced following their washing.
5th Workshop: code No. P025

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	P8590	SAB5600061	SAB4700466
Sigma-Aldrich F8402 Monoclonal Anti-CD31 (PECAM-1)−FITC antibody produced in mouse	Sigma-Aldrich P8590 Anti-CD31(PECAM-1) antibody , Mouse monoclonal	Sigma-Aldrich SAB5600061 Anti-CD31 (PECAM-1) antibody, Rabbit monoclonal	Sigma-Aldrich SAB4700466 Monoclonal Anti-CD31-FITC antibody produced in mouse
description clone WM-59, purified immunoglobulin, buffered aqueous solution	description clone WM-59, purified from hybridoma cell culture	description recombinant, expressed in HEK 293 cells, clone RM247, purified immunoglobulin	description clone MEM-05, purified immunoglobulin, buffered aqueous solution
conjugate FITC conjugate	conjugate unconjugated	conjugate unconjugated	conjugate FITC conjugate
clone WM-59, monoclonal	clone WM-59, monoclonal	clone recombinant monoclonal, RM247, monoclonal	clone MEM-05, monoclonal
antibody form purified immunoglobulin	antibody form purified from hybridoma cell culture	antibody form purified immunoglobulin	antibody form purified immunoglobulin
species reactivity human	species reactivity human	species reactivity human	species reactivity human
biological source mouse	biological source mouse	biological source -	biological source mouse
Gene Information human ... PECAM1(5175)	Gene Information human ... PECAM1(5175)	Gene Information human ... PECAM1(5175)	Gene Information human ... PECAM1(5175)

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Storage Class

10 - Combustible liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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