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F8799

Fas/Fc Chimera from mouse

>97% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

Synonym(s):

Apo-1, CD95

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50 μg
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$695.00
$590.75

About This Item

UNSPSC Code:
12352200
NACRES:
NA.32
MDL number:
MFCD01867037

$590.75

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recombinant

expressed in baculovirus infected Sf21 cells

Quality Level

200

assay

>97% (SDS-PAGE)

form

lyophilized powder

potency

0.03-0.1 μg per mL ED50 (in the presence of 20 ng/mL recombinant human Fas ligand for cytotoxicty assay of Jurkat cells)

mol wt

55 kDa (glycosylated form)

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

P25446

storage temp.

−20°C

Gene Information

mouse ... Fas(14102)

General description

Fas is a key component of apoptosis, and is a cell surface receptor. This gene is localized to human chromosome 10q24.1, and is composed of nine exons and eight introns.[1]

Application

Fas/Fc Chimera from mouse has been used as a blocking agent during FACS (flow cytometry).[2]

Biochem/physiol Actions

Fas and its ligand play an essential role in apoptosis, by activating the cell death signaling pathway. Mutations in this gene are associated with evasion of apoptosis by cells, leading to tumorigenesis, and prevention of clearance of these cells by the immune system.[1] A polymorphism in this gene results in the production of soluble Fas (sFas), which is implicated in the chronic autoimmune disorder called primary Sjögren′s syndrome (pSS).[3]

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 50 μg bovine serum albumin per 1 μg of cytokine.

Analysis Note

Biological activity was measured by its ability to inhibit Fas-ligand induced apoptosis of Jurkat cells.

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Fas/Fc Chimera from mouse >97% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

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R0775

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Fas Intracellular Domain human >90% (SDS-PAGE), recombinant, expressed in E. coli (N-terminal histidine tagged), buffered aqueous solution

Sigma-Aldrich

F2053

Fas Intracellular Domain human

Ephrin-A1/Fc Chimera from mouse >90% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

Sigma-Aldrich

E9902

Ephrin-A1/Fc Chimera from mouse

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in NSO cells

recombinant

expressed in E. coli (N-terminal histidine tagged)

recombinant

expressed in NSO cells

assay

>97% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

>90% (SDS-PAGE)

assay

>90% (SDS-PAGE)

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

lyophilized powder

form

lyophilized powder

form

buffered aqueous solution

form

lyophilized powder

Gene Information

mouse ... Fas(14102)

Gene Information

mouse ... Tnfrsf11a(21934)

Gene Information

human ... FAS(355)

Gene Information

mouse ... Efna1(13636)

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
091M1506
081M1218
061M1220
129K2178
010M1089

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Related Content

Data Sheet - F8799

Instructions

Davidson D Ateh et al.
Biomaterials, 32(33), 8538-8547 (2011-08-10)
The CD95/CD95L receptor-ligand system is mainly recognised in the induction of apoptosis. However, it has also been shown that CD95L is over-expressed in many cancer types where it modulates immune-evasion and together with its receptor CD95 promotes tumour growth. Here
J Cheng et al.
Science (New York, N.Y.), 263(5154), 1759-1762 (1994-03-25)
Fas is an apoptosis-signaling receptor molecule on the surface of a number of cell types. Molecular cloning and nucleotide sequence analysis revealed a human Fas messenger RNA variant capable of encoding a soluble Fas molecule lacking the transmembrane domain because
A Oehm et al.
The Journal of biological chemistry, 267(15), 10709-10715 (1992-05-25)
The APO-1 antigen as defined by the mouse monoclonal antibody anti-APO-1 was previously found to be expressed on the cell surface of activated human T and B lymphocytes and a variety of malignant human lymphoid cell lines. Cross-linking of the
View All Related Papers

Global Trade Item Number

SKUGTIN
F8799-50UG04061833384800

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