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Anti-Fatty Acid Synthase (C-terminal) antibody produced in rabbit

Name: Anti-Fatty Acid Synthase (C-terminal) antibody produced in rabbit
Brand: SIGMA
Price: 614 USD

1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-FAS, Anti-FASN

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Pack Size	SKU	Availability	Price
200 μL		Check Cart for Availability	$614.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00283209

Conjugate:

unconjugated

Clone:

polyclonal

Application:

Citations:

$614.00

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Properties

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 270 kDa

species reactivity

mouse, rat, human

concentration

1 mg/mL

technique(s)

western blot: 0.5-1 μg/mL using lysates of Rat1 cells, western blot: 1-2 μg/mL using lysates of human HEK 293-T cells

UniProt accession no.

P49327

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... FASN(2194)
mouse ... Fasn(14104)
rat ... Fasn(50671)

Description

General description

Fatty acid synthase (FAS, FASN) is a multifunctional protein, mainly involved in catalyzing the synthesis of palmitate from acetyl-CoA and malonyl CoA, in the presence of NADPH. In mammals, it is composed of two identical multifunctional polypeptides, in which three catalytic domains in the N-terminal section (β-ketoacyl synthase, malonyl/acetyl tansferase, and dehydrase) are separated by a core region of about 600 residues from four C-terminal domains (enoyl reductase, β-ketoacyl reductase, acyl carrier protein, and thioesterase).

Immunogen

synthetic peptide corresponding to amino acids 2497-2511 of human fatty acid synthase, conjugated to KLH via an N-terminal added lysine residue. The corresponding sequence is conserved in human, rat, and mouse.

Application

Anti-Fatty Acid Synthase (C-Terminal) antibody is suitable for western blot analysis at a concentration of 0.5-1μg/mL using lysates of Rat1 cells and 1-2μg/mL using lysates of human HEK 293-T cells.

Biochem/physiol Actions

FAS plays an essential role during embryogenesis and energy homeostasis in adult animals. It also acts as the carbon source for fatty acid synthesis. It has been shown to be overexpressed in various types of human cancer, including prostate, breast, ovary and others.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	Y0396	SAB1403807	WH0002194M1
Sigma-Aldrich F9679 Anti-Fatty Acid Synthase (C-terminal) antibody produced in rabbit	Sigma-Aldrich Y0396 Anti-YB-1 (C-terminal) antibody produced in rabbit	Sigma-Aldrich SAB1403807 Monoclonal Anti-FASN antibody produced in mouse	Sigma-Aldrich WH0002194M1 Monoclonal Anti-FASN antibody produced in mouse
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form purified immunoglobulin	antibody form purified immunoglobulin
Quality Level 200	Quality Level 200	Quality Level 100	Quality Level 100
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
biological source rabbit	biological source rabbit	biological source mouse	biological source mouse
technique(s) western blot: 0.5-1 μg/mL using lysates of Rat1 cells, western blot: 1-2 μg/mL using lysates of human HEK 293-T cells	technique(s) immunoprecipitation (IP): 5-10 μg using HEK-293T cell lysates, indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde-fixed NIH-3T3 cells, western blot: 0.5-1 μg/mL using PC12 cell lysates	technique(s) indirect ELISA: suitable, indirect immunofluorescence: suitable, western blot: 1-5 μg/mL	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, indirect immunofluorescence: suitable, western blot: 1-5 μg/mL
clone polyclonal	clone polyclonal	clone 3B3-1D6, monoclonal	clone 3F2-1F3, monoclonal

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number

097M4800V

081M4772

086K4817

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Articles

Fatty Acid Synthesis in Cancer Cells

Fatty acid synthesis supports cancer cell proliferation, essential for membrane generation, protein modification, and bioenergetics.

Involvement of a unique carbohydrate-responsive factor in the glucose regulation of rat liver fatty-acid synthase gene transcription.

C Rufo et al.

The Journal of biological chemistry, 276(24), 21969-21975 (2001-03-30)

Refeeding carbohydrate to fasted rats induces the transcription of genes encoding enzymes of fatty acid biosynthesis, e.g. fatty-acid synthase (FAS). Part of this transcriptional induction is mediated by insulin. An insulin response element has been described for the fatty-acid synthase

Structure and molecular organization of mammalian fatty acid synthase.

Francisco J Asturias et al.

Nature structural & molecular biology, 12(3), 225-232 (2005-02-16)

De novo synthesis of fatty acids in the cytosol of animal cells is carried out by the multifunctional, homodimeric fatty acid synthase (FAS). Cryo-EM analysis of single FAS particles imaged under conditions that limit conformational variability, combined with gold labeling

Proteomic study reveals that proteins involved in metabolic and detoxification pathways are highly expressed in HER-2/neu-positive breast cancer.

DaoHai Zhang et al.

Molecular & cellular proteomics : MCP, 4(11), 1686-1696 (2005-07-29)

The receptor tyrosine kinase ErbB2 (HER-2/neu) is overexpressed in up to 30% of breast cancers and is associated with poor prognosis and an increased likelihood of metastasis especially in node-positive tumors. In this proteomic study, to identify the proteins that

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Global Trade Item Number

SKU	GTIN
F9679-200UL	04061838105400

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