FCANOS1
Nitric Oxide Synthase Detection System, Fluorimetric
sufficient for 200 reactions
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| Size/SKU | Availability | Price |
|---|---|---|
1 kit | Check Cart for Availability | $833.00 |
About This Item
$833.00
usage
sufficient for 200 reactions
Quality Segment
availability
not available in Japan
shipped in
wet ice
storage temp.
2-8°C
Gene Information
human ... NOS1(4842), NOS2(4843), NOS2B(201288), NOS2C(645740), NOS3(4846)
Biochem/physiol Actions
The standard assay for NOS activity is for solubilized cell-free enzyme preparations such as cell lysates, crude tissue extracts or purified enzymes. It measures the production of radiolabeled citrulline from radiolabeled arginine substrate (Sigma Product No. NOS-1). In contrast, the Fluorometric Cell-Associated NOS Detection System measures intracellular production of NO by a non-radiometric method. Radioisotopes are replaced by a cell-permeable diacetate derivative of 4,5-diaminofluorescein (DAF-2 DA). DAF-2 DA penetrates cells rapidly where it is hydrolyzed by intracellular esterase to DAF-2 that, in turn, reacts with NO produced by NOS to form a fluorescent triazolo-fluorescein. The fluorescent product can be quantitated using an excitation filter at 492 nm and an emission filter at 515 nm.
Legal Information
US Patent No. 5,874, 590
1 of 1
This Item | |||
|---|---|---|---|
| description sufficient for 200 reactions | description Supplied with 1 Reader, 1 Reader Stand, 1 Membrane Transfer Tool, 1 Removal Rack, 1 Camera and Installation flash drive | description recombinant, expressed in E. coli, buffered aqueous solution | description clone NOS-IN, purified from hybridoma cell culture |
| usage sufficient for 200 reactions | usage - | usage - | usage - |
| shipped in wet ice | shipped in - | shipped in dry ice | shipped in dry ice |
| Quality Level 200 | Quality Level EQ2 | Quality Level 200 | Quality Level 200 |
| availability not available in Japan | availability - | availability - | availability - |
| storage temp. 2-8°C | storage temp. - | storage temp. −70°C | storage temp. −20°C |
| Gene Information human ... NOS1(4842), NOS2(4843), NOS2B(201288), NOS2C(645740), NOS3(4846) | Gene Information - | Gene Information mouse ... Nos2(18126) | Gene Information mouse ... Nos2(18126) |
Kit Components Only
- Diphenyleneiodonium Chloride (DPI) 1 mM 200 μL
- Arginine Substrate Solution for NOS Detection, Fluorimetric 2 mL
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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