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G1160

Monoclonal Anti-Glutathione-S-Transferase (GST) antibody produced in mouse

clone GST-2, ascites fluid

Synonym(s):

Mouse Anti-GST Tag

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100 μL

Available to ship TODAYfromMILWAUKEE

$552.00
0.2 mL

Available to ship TODAYfromMILWAUKEE

$727.00
$617.95

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00212963
Conjugate:
unconjugated
Clone:
GST-2, monoclonal
Application:
DB, ELISA (i), WB
Citations:
104

$552.00

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biological source

mouse

Quality Segment

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

GST-2, monoclonal

contains

15 mM sodium azide

technique(s)

dot blot: suitable, indirect ELISA: suitable, western blot: 1:1,000 using purified recombinant GST or lysate of induced bacteria expressing recombinant GST

isotype

IgG2b

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Monoclonal anti-glutathione-S-transferase (GST) (mouse IgG2b isotype) is derived from the GST-2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a purified recombinant GST fusion protein.GST belongs to three family of proteins distinguished as cytosolic, mitochondrial and microsomal GST. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: α, κ, μ, ω, σ, θ, π and ζ.
Recombinant target proteins are often expressed as a fusion product with Glutathione-S-Transferase (GST) tags using various expression vector constructs. Thus, antibodies directed against the GST tags of the recombinant constructs can facilitate the purification and study of target proteins. Monoclonal Anti-Glutathione-S-Transferase (GST) antibody reacts with GST from Schistosoma japonicum. Furthermore, the product identifies native as well as denatured-reduced forms of purified GST and GST fusion proteins. The antibody does not detect GST derived from rat, rabbit, porcine and bovine liver or from human placenta.

Immunogen

recombinant Glutathione-S-Transferase (GST) fusion protein.

Application

Monoclonal Anti-Glutathione-S-Transferase (GST) antibody has been used for use in western blot and ELISA. This product has also been used for dot blot.

Biochem/physiol Actions

Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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G7781G6539H1029
Monoclonal Anti-Glutathione-S-Transferase (GST) antibody produced in mouse clone GST-2, ascites fluid

Sigma-Aldrich

G1160

Monoclonal Anti-Glutathione-S-Transferase (GST) antibody produced in mouse

Anti-Glutathione-S-Transferase (GST) antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

Sigma-Aldrich

G7781

Anti-Glutathione-S-Transferase (GST) antibody produced in rabbit

Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse clone GFP-20, ascites fluid

Sigma-Aldrich

G6539

Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse

Monoclonal Anti-polyHistidine antibody produced in mouse clone HIS-1, ascites fluid

Sigma-Aldrich

H1029

Monoclonal Anti-polyHistidine antibody produced in mouse

description

clone GST-2, ascites fluid

description

IgG fraction of antiserum, buffered aqueous solution

description

clone GFP-20, ascites fluid

description

clone HIS-1, ascites fluid

biological source

mouse

biological source

rabbit

biological source

mouse

biological source

mouse

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

ascites fluid

antibody form

IgG fraction of antiserum

antibody form

ascites fluid

antibody form

ascites fluid

clone

GST-2, monoclonal

clone

polyclonal

clone

GFP-20, monoclonal

clone

HIS-1, monoclonal

isotype

IgG2b

isotype

-

isotype

IgG1

isotype

IgG2a

technique(s)

dot blot: suitable, western blot: 1:1,000 using purified recombinant GST or lysate of induced bacteria expressing recombinant GST, indirect ELISA: suitable

technique(s)

indirect ELISA: 1:10,000, western blot: 1:2,000 using lysates of induced Escherichia coli expressing GST, dot blot: suitable

technique(s)

western blot: 1:2,000 using purified recombinant GFP preparation, indirect ELISA: suitable, dot blot: suitable

technique(s)

indirect ELISA: suitable, dot blot: suitable, immunoprecipitation (IP): suitable, western blot: 1:3,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein

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12 - Non Combustible Liquids

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