The K12 strain information can be found on the UniProt website under the following entry: P0A9C5.
G1270
L-Glutamine Synthetase from Escherichia coli
lyophilized powder, 400-2,000 units/mg protein
Synonym(s):
L-Glutamate:ammonia ligase (ADP-forming)
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| Size/SKU | Availability | Price |
|---|---|---|
100 units | Check Cart for Availability | $715.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.26
EC Number:
MDL number:
Specific activity:
400-2,000 units/mg protein
form
lyophilized powder
Quality Segment
specific activity
400-2,000 units/mg protein
purified by
affinity chromatography
contains
dithioerythritol as preservative
composition
Protein, ~5% Lowry
solubility
H2O: soluble 0.95-1.05 mg/mL, clear to hazy
UniProt accession no.
foreign activity
ATPase <0.2%
storage temp.
−20°C
Gene Information
Escherichia coli K12 ... glnA(948370)
General description
L-Glutamine Synthetase from bacteria shows dodecameric structure comprising of 12 active sites. Each active site termed bifunnel, has an ATP and glutamate binding sites. The dodecamer is stabilized by two hexameric rings.
Application
L-Glutamine Synthetase from Escherichia coli has been used in the synthesis of methylglutamine from methylammonium in E coli and in the glutamine synthetase protection activity of human thioredoxin peroxidase enzyme, AOE372.
Biochem/physiol Actions
L-glutamine synthetase catalyzes the condensation of L-glutamate and ammonia to L-glutamine. It is a degradative enzyme for glutamic acid.
Degradative enzyme for glutamic acid
Nitrogen starvation dictates the expression of the glutamine synthetase (GS) gene in E. coli. GS plays a key role in ammonia assimilation in bacteria. Adenylylation of GS is catalyzed by adenylyltransferase. Adenylylation of GS modulates its catalytic functionality resulting in glutamine limitation in E coli.
Physical form
Contains potassium phosphate, sodium citrate and magnesium acetate buffer salts
Other Notes
One unit will convert 1.0 μmole of L-glutamate to L-glutamine in 15 min at pH 7.1 at 37 °C.
1 of 1
This Item | ||
|---|---|---|
| description lyophilized powder, 400-2,000 units/mg protein | description Grade V, lyophilized powder, ≥50 units/mg protein | description lyophilized powder, ≥150 units/mg protein (biuret) |
| Gene Information Escherichia coli K12 ... glnA(948370) | Gene Information - | Gene Information Escherichia coli K12 ... ackA(946775) |
| specific activity 400-2,000 units/mg protein | specific activity ≥50 units/mg protein | specific activity ≥150 units/mg protein (biuret) |
| form lyophilized powder | form lyophilized powder | form lyophilized powder |
| solubility H2O: soluble 0.95-1.05 mg/mL, clear to hazy | solubility water: 1 mg/mL (Clear w/Particle to Sl. Hazy w/Particles) | solubility - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| UniProt accession no. | UniProt accession no. - | UniProt accession no. |
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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From which E. coli strain is your product G1270 L-Glutamine Synthetase from Escherichia coli isolated?
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