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Glycerol Dehydrogenase from Cellulomonas sp.

Name: Glycerol Dehydrogenase from <I>Cellulomonas</I> sp.
Brand: SIGMA

lyophilized powder, ≥50 units/mg protein

Synonym(s):

Glycerol:NAD⁺ 2-oxidoreductase

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About This Item

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-824-3

MDL number:

MFCD00131206

EC Number:

1.1.1.6 (BRENDA, IUBMB)

Specific activity:

≥50 units/mg protein

Biological source:

bacterial (Cellulomonas spp.)

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Properties

biological source

bacterial (Cellulomonas spp.)

Quality Segment

200

form

lyophilized powder

specific activity

≥50 units/mg protein

mol wt

~390 kDa

composition

Protein, ≥60% biuret

storage temp.

−20°C

Description

General description

Glycerol Dehydrogenase belongs to iron-containing alcohol dehydrogenase family. It is made up of eight identical subunits which are about 42,000 Da each. The C-terminal has histidine and the N-terminal has serine amino acid.

Isoelectric point : 4.4 ± 0.1
Michaelis constants : 1.1 x 10¯²M (Glycerol), 8.9 x 10¯⁵M (NAD⁺⁾
Structure : 10 subunits (42,000) per mol of enzyme
Inhibitors : p-Chloromercuribenzoate, o-phenanthroline, monoiodoacetate, heavy metal
ions (Co++, Ni⁺⁺, Cu⁺⁺, Zn⁺⁺, Cd⁺⁺)
Optimum pH : 10.0 – 10.5
Optimum temperature : 50°C
pH Stability : pH 7.5 – 10.5 (25°C, 20hr)
Thermal stability : below 55°C (pH 7.5, 15min)
Substrate specificity : This enzyme has the highest specificity for glycerol and 1,2-propanediol, and also oxidizes glycerol-α-monochlorohydrin, ethylene
glycol and 2,3-butanediol. The oxidative reaction is stimulated by K⁺, NH⁺4 and Rb⁺.

Application

Glycerol Dehydrogenase (GDH) from Cellulomonas sp. has been used in the determination of glycerol by sequential injection analysis (SIA) method. It has also been used in the preparation of pyrroloquinoline quinone (PQQ) based biosensor for the determination of glycerol.

This enzyme is useful for enzymatic determination of glycerol and of triglyceride when coupled with lipoprotein lipase in clinical analysis. Formation of NADH from the reaction of glycerol and NAD⁺ was catalyzed by the enzyme glycerol dehydrogenase.

Biochem/physiol Actions

Glycerol dehydrogenase catalyzes the conversion of glycerol to glycerone.

Physical form

Lyophilized powder containing bovine serum albumin

Other Notes

One unit will oxidize 1.0 μmole of glycerol to dihydroxyacetone per min at 25 °C at pH 10.0.

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This Item	G6142	L5135	P4553
Sigma-Aldrich G3512 Glycerol Dehydrogenase from Cellulomonas sp.	Sigma-Aldrich G6142 Glycerokinase from Cellulomonas sp.	Sigma-Aldrich L5135 L-Leucine Dehydrogenase from Bacillus cereus	Sigma-Aldrich P4553 6-Phosphogluconic Dehydrogenase from yeast
description lyophilized powder, ≥50 units/mg protein	description lyophilized powder, 25-75 units/mg protein	description lyophilized powder, ≥60 units/mg protein	description lyophilized powder, ≥3.0 units/mg solid
specific activity ≥50 units/mg protein	specific activity 25-75 units/mg protein	specific activity ≥60 units/mg protein	specific activity ≥3.0 units/mg solid
biological source bacterial (Cellulomonas spp.)	biological source -	biological source -	biological source yeast
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C
mol wt ~390 kDa	mol wt ~128 kDa (by gel filtration)	mol wt 245 kDa	mol wt 52 kDa
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200

Pictograms

GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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