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G4252

Glyoxalase I from Saccharomyces cerevisiae

Grade IV, buffered aqueous glycerol solution, ≥400 units/mg protein

Synonym(s):

S-Lactoyl-glutathione methylglyoxal-lyase (isomerizing)

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Size/SKUAvailabilityPrice
200 units

Available to ship TODAYfromMILWAUKEE

$161.00
500 units

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$319.00
2500 units

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$1,180.00

About This Item

CAS Number:
9033-12-9
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
4.4.1.5 (BRENDA, IUBMB)
MDL number:
MFCD00131225
Specific activity:
≥400 units/mg protein

$161.00

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type

Grade IV

Quality Segment

form

buffered aqueous glycerol solution

specific activity

≥400 units/mg protein

foreign activity

glyoxalase II ≤1%

storage temp.

2-8°C

General description

Glyoxalase detoxification system consists of glyoxalase (GLO)-I and GLO-II. GLO-I is a cytosolic, 42 kDa, dimeric Zn2+ metalloenzyme.

Application

Glyoxalase I from Saccharomyces cerevisiae has been used as a standard in Glyoxalase I assay for C. dilatata females and brooded encapsulated embryos. It has also been used as a standard for calibration curve generation for quantifying glyoxalase I from brain tissues and cerebral microvessels.

Biochem/physiol Actions

Glyoxalase I is universally expressed and involved in the protection against cellular damage due to cytotoxic metabolites such as advanced glycation end products (AGEs). It is an integral component of the detoxification system, catalyzing the conversion of reactive, acyclic a-oxoaldehydes into the corresponding a-hydroxyacids in a glutathione-dependent manner.

Physical form

Solution in 50% glycerol, 0.4 M (NH4)2SO4 and 0.002 M KH2PO4 pH 6.5

Analysis Note

Protein determined by biuret.

Other Notes

One unit will form 1.0 μmole of S-lactoylglutathione from methylglyoxal and reduced glutathione per min at pH 6.6 at 25 °C.

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This Item
A8656C2436346210
Glyoxalase I from Saccharomyces cerevisiae Grade IV, buffered aqueous glycerol solution, ≥400 units/mg protein

Sigma-Aldrich

G4252

Glyoxalase I from Saccharomyces cerevisiae

Alcohol Dehydrogenase from Saccharomyces cerevisiae

Sigma-Aldrich

A8656

Alcohol Dehydrogenase from Saccharomyces cerevisiae

Cytochrome c from Saccharomyces cerevisiae ≥85% based on Mol. Wt. 12,588 basis

Sigma-Aldrich

C2436

Cytochrome c from Saccharomyces cerevisiae

β-Glucan, Saccharomyces cerevisiae A major structural element of the yeast cell wall having a β1,3-glucan linear structure with a small number of β1,6-glucan branches. Has been shown to induce an oxidative burst response from NR8383 cells.

Millipore

346210

β-Glucan, Saccharomyces cerevisiae

description

Grade IV, buffered aqueous glycerol solution, ≥400 units/mg protein

description

-

description

≥85% based on Mol. Wt. 12,588 basis

description

A major structural element of the yeast cell wall having a β1,3-glucan linear structure with a small number of β1,6-glucan branches. Has been shown to induce an oxidative burst response from NR8383 cells.

specific activity

≥400 units/mg protein

specific activity

-

specific activity

-

specific activity

-

form

buffered aqueous glycerol solution

form

powder

form

powder

form

solid

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

15-25°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

foreign activity

glyoxalase II ≤1%

foreign activity

-

foreign activity

-

foreign activity

-

type

Grade IV

type

-

type

-

type

-

Storage Class

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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