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Anti-Guanylyl Cyclase α1 antibody produced in rabbit

Name: Anti-Guanylyl Cyclase α1 antibody produced in rabbit
Brand: SIGMA
Price: 666 USD
Availability: InStock

IgG fraction of antiserum, buffered aqueous solution

Enhanced Validation

Synonym(s):

Anti-GC-S-alpha-1, Anti-GC-SA3, Anti-GCS-alpha-3, Anti-GUC1A3, Anti-GUCA3, Anti-GUCSA3, Anti-GUCY1A3, Anti-MYMY6

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Size/SKU	Availability	Price
0.2 mL	Check Cart for Availability	$666.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD03455075

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IHC (p), IP, WB

Citations:

$666.00

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

~80 kDa

species reactivity

mouse, rat, human, bovine

packaging

antibody small pack of 25 μL

enhanced validation

independent
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technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using trypsin-treated human, bovine and mouse heart tissue, immunoprecipitation (IP): 5-10 μg using 60-120 μg of a cytosolic fraction of rat brain, western blot: 1:10,000 using cytosolic fraction of rat brain

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GUCY1A3(2982)
mouse ... Gucy1a3(60596)
rat ... Gucy1a3(497757)

Description

General description

The enzyme consists of α and β subunits of ~80kDa and ~70kDa respectively. Both the units are required for catalytic activity. The N-terminal domains of the subunits are essential for the stimulation of the enzyme by NO. Dimerization is mediated by the central portion of GC. The C-terminus domain of both subunits forms the catalytic domain.

Immunogen

synthetic peptide corresponding to amino acid residues 673-690 of rat soluble Guanylyl cyclase α1, conjugated to KLH with glutaraldehyde. The corresponding human sequence differs by 2 amino acids.

Application

Anti-Guanylyl Cyclase α1 antibody produced in rabbit is suitable for immunohistochemistry at a working dilution of 1:100 using trypsin-treated human, bovine and mouse heart tissue and immunoprecipitation at 5-10μg concentration using 60-120μg of a cytosolic fraction of rat brain. It is also suitable for western blot analysis using cytosolic fraction of rat brain at a working dilution of 1:10,000.
It was used as a primary antibody for immunohistochemical:

localization of α1 subunits of sGC (soluble guanylate cyclase) in the guinea pig gastrointestinal tract
detection of expression of sGC in the vasculature of rat skeletal muscle
localization of the functional subunit of NO receptors, sGCα1 in guinea pig caecum

Biochem/physiol Actions

Guanylyl cyclase (GC) catalyzes the conversion of guanosine-5′-triphosphate (GTP) to cyclic guanosine-3′,5-monophosphate (cGMP) and pyrophosphate. This reaction requires Mg²⁺ or Mn²⁺.

Guanylyl cyclase (GC) catalyzes the conversion of guanosine-5′-triphosphate (GTP) to cyclic guanosine-3′,5-monophosphate (cGMP) and pyrophosphate. This reaction requires Mg²⁺ or Mn²⁺.†† The enzyme (GC) is a major physiological receptor for nitric oxide (NO), an important intra- and intercellular membrane-permeant signaling molecule. Gaseous NO binds to Fe²⁺ in the prosthetic heme group of the enzyme. NO binding is followed by disruption of the β1 subunit histidine¹⁰⁵ bond to iron and activation of the enzyme. GC forms a complex with NO and cGMP and regulates smooth muscle relaxation, inflammation, platelet adhesion and aggregation, pulmonary physiology and neuronal function. It is an important target for NO-releasing and non-NO-releasing activator drugs in human cardiovascular therapy.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	G4405	V2514	HPA058617
$Anti-Guanylyl Cyclase α1 antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich G4280 Anti-Guanylyl Cyclase α1 antibody produced in rabbit	Sigma-Aldrich G4405 Anti-Guanylyl Cyclase β1 (ER-19) antibody produced in rabbit	$Anti-VGLUT2 (HY-19) antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich V2514 Anti-VGLUT2 (HY-19) antibody produced in rabbit	Sigma-Aldrich HPA058617 Anti-GUCY1A3 antibody produced in rabbit
description IgG fraction of antiserum, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution	description IgG fraction of antiserum, buffered aqueous solution	description Prestige Antibodies^® Powered by Atlas Antibodies, affinity isolated antibody
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level -
antibody form IgG fraction of antiserum	antibody form affinity isolated antibody	antibody form IgG fraction of antiserum	antibody form affinity isolated antibody
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
Gene Information human ... GUCY1A3(2982) mouse ... Gucy1a3(60596) rat ... Gucy1a3(497757)	Gene Information human ... GUCY1B3(2983) mouse ... Gucy1b3(54195) rat ... Gucy1b3(25202)	Gene Information human ... SLC17A6(57084) mouse ... Slc17a6(140919) rat ... Slc17a6(84487)	Gene Information human ... GUCY1A3(2982)
technique(s) western blot: 1:10,000 using cytosolic fraction of rat brain, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using trypsin-treated human, bovine and mouse heart tissue, immunoprecipitation (IP): 5-10 μg using 60-120 μg of a cytosolic fraction of rat brain	technique(s) western blot: 1:2,000 using cytosolic fraction of rat brain, immunoprecipitation (IP): 2-3 μg using 60-120 μg of a cytosolic fraction of rat brain, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 using trypsin-digested human, bovine and mouse heart tissue	technique(s) western blot: 1:2,000 using synaptic vesicle (SV) fraction of rat brain	technique(s) immunofluorescence: 0.25-2 μg/mL

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Storage Class

12 - Non Combustible Liquids

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WGK 1

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