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Anti-Guanylyl Cyclase β1 (ER-19) antibody produced in rabbit

Name: Anti-Guanylyl Cyclase β1 (ER-19) antibody produced in rabbit
Brand: SIGMA
Price: 575.45 USD
Availability: InStock

affinity isolated antibody, buffered aqueous solution

Enhanced Validation

Synonym(s):

Anti-GC-S-beta-1, Anti-GC-SB3, Anti-GUC1B3, Anti-GUCB3, Anti-GUCSB3, Anti-GUCY1B3

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Size/SKU	Availability	Price
0.2 mL	Check Cart for Availability	$677.00 $575.45

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD03455078

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IHC (p), IP, WB

Citations:

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 70 kDa

species reactivity

rat, mouse, human, bovine

enhanced validation

independent
Learn more about Antibody Enhanced Validation

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 using trypsin-digested human, bovine and mouse heart tissue, immunoprecipitation (IP): 2-3 μg using 60-120 μg of a cytosolic fraction of rat brain, western blot: 1:2,000 using cytosolic fraction of rat brain

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GUCY1B3(2983)
mouse ... Gucy1b3(54195)
rat ... Gucy1b3(25202)

Description

General description

Soluble guanylyl cyclase (sGC) is an obligate hemoprotein enzyme consisting of α and β subunits of ~80 kDa and ~70 kDa, respectively.

Immunogen

synthetic peptide corresponding to amino acid residues 189-207, with N-terminal added lysine, of rat soluble guanylate cyclase 1, conjugated to KLH with glutaraldehyde. The sequence differs in human, bovine and mouse by 1 amino acid.

Application

Anti-Guanylyl Cyclase β1 (ER-19) antibody produced in rabbit is suitable for immunoblotting at a working dilution of 1:2000 using a cytosolic fraction of rat brain, immunoprecipitation at a working antibody amount of 2-3μg using 60-120μg of a cytosolic fraction of rat brain and for immunohistochemistry at 1:400 using trypsin-digested human, bovine and mouse heart tissue.
It has been used as a primary antibody for:

localization of β1 subunits of sGC (soluble guanylate cyclase) in the guinea pig gastrointestinal tract
detection of expression of sGC in the vasculature of rat skeletal muscle
localization of the functional subunit of NO receptors, sGCβ1 in guinea pig caecum

It has also been used in immunoblot assay to determine whether there were changes in uterine vascular smooth muscle (UVSM) sGC expression during the ovine reproductive cycle.

Biochem/physiol Actions

Guanylyl cyclase (GC) catalyzes the conversion of guanosine-5′–triphosphate (GTP) to cyclic guanosine-3′,5-monophosphate (cGMP) and pyrophosphate. This reaction requires Mg²⁺ or Mn²⁺. Both the units are required for catalytic activity. The N-terminal domains of the subunits are essential for the stimulation of the enzyme by NO. Dimerization is mediated by the central portion of GC. The C-terminus domain of both subunits forms the catalytic domain.

The enzyme (GC) is a major physiological receptor for nitric oxide (NO), an important intra- and intercellular membrane-permeant signaling molecule. Gaseous NO binds to Fe²⁺ in the prosthetic heme group of the enzyme. NO binding is followed by disruption of the β1 subunit histidine¹⁰⁵ bond to iron and activation of the enzyme. GC forms a complex with NO and cGMP and regulates smooth muscle relaxation, inflammation, platelet adhesion and aggregation, pulmonary physiology and neuronal function. It is an important target for NO-releasing and non-NO-releasing activator drugs in human cardiovascular therapy.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Show Differences

1 of 1

This Item	G4280	MABN2746	SAB5701478
Sigma-Aldrich G4405 Anti-Guanylyl Cyclase β1 (ER-19) antibody produced in rabbit	$Anti-Guanylyl Cyclase α1 antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich G4280 Anti-Guanylyl Cyclase α1 antibody produced in rabbit	MABN2746 Anti-Guanylate cyclase 1 Antibody, clone IS4	Sigma-Aldrich SAB5701478 Anti-Guanylyl Cyclase beta 1 (GUCY1B3) antibody produced in rabbit
description affinity isolated antibody, buffered aqueous solution	description IgG fraction of antiserum, buffered aqueous solution	description -	description -
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 100
biological source rabbit	biological source rabbit	biological source mouse	biological source rabbit
antibody form affinity isolated antibody	antibody form IgG fraction of antiserum	antibody form purified antibody	antibody form -
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
technique(s) immunoprecipitation (IP): 2-3 μg using 60-120 μg of a cytosolic fraction of rat brain, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 using trypsin-digested human, bovine and mouse heart tissue, western blot: 1:2,000 using cytosolic fraction of rat brain	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using trypsin-treated human, bovine and mouse heart tissue, western blot: 1:10,000 using cytosolic fraction of rat brain, immunoprecipitation (IP): 5-10 μg using 60-120 μg of a cytosolic fraction of rat brain	technique(s) immunohistochemistry: suitable, western blot: suitable, immunofluorescence: suitable	technique(s) immunohistochemistry: 1:50-1:200, western blot: 1:500-1:2000
shipped in dry ice	shipped in dry ice	shipped in -	shipped in wet ice

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Storage Class

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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