G6142
Glycerokinase from Cellulomonas sp.
lyophilized powder, 25-75 units/mg protein
Synonym(s):
glpK, ATP:glycerol 3-phosphotransferase, Glycerol Kinase
Select a Size
| Size/SKU | Availability | Price |
|---|---|---|
1000 units | Available to ship TODAYfromMILWAUKEE | $393.00 |
About This Item
form
lyophilized powder
Quality Segment
specific activity
25-75 units/mg protein
mol wt
~128 kDa (by gel filtration)
composition
Protein, ≥60% biuret
storage temp.
−20°C
General description
Michaelis constants : 4.4 x 10-5M (Glycerol), 4.3 x 10-4M (ATP)
Inhibitors : p-Chloromercuribenzoate, heavy metal ions (Pb++, Fe++, Hg++, Ag+)
Optimum pH : 9.8 (G-3-PDH system), 7.8 (G-3-P oxidase system) Optimum temperature : 500C
pH Stability : pH 5.5 x 10.0 (25oC, 20hr)
Thermal stability : below 40oC (pH 7.5, 15min)
Substrate specificity : This enzyme catalyzes the stereospecific transfer of the terminal
phosphoryl moiety of ATP to one of the primary hydroxyl group of
glycerol, forming sn-glycerol-3-P. The enzyme has the highest
specificity for glycerol, and also phosphorylates dihydroxyacetone
and glyceraldehyde (Table 1,2). Mg++ is essentially required for the
reaction.
Application
- for determining the kinetic characteristics of human and trypanosomatid phosphofructokinases using an enzyme-linked kinetic assay.
- to study the effect of sugar in fluorescence emission.
- in 2-Arachidonoylglycerol-based fluorescence assay for DH-463, a fluorescent activity-based probe for monoacylglycerol lipase.
Biochem/physiol Actions
Physical form
Other Notes
1 of 1
This Item | |||
|---|---|---|---|
| description lyophilized powder, 25-75 units/mg protein | description lyophilized powder, 40-100 units/mg protein | description buffered aqueous solution, ≥75 units/mg protein (biuret) | description lyophilized powder, ≥150 units/mg protein (biuret) |
| specific activity 25-75 units/mg protein | specific activity 40-100 units/mg protein | specific activity ≥75 units/mg protein (biuret) | specific activity ≥150 units/mg protein (biuret) |
| form lyophilized powder | form lyophilized powder | form buffered aqueous solution | form lyophilized powder |
| mol wt ~128 kDa (by gel filtration) | mol wt - | mol wt - | mol wt - |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| composition Protein, ≥60% biuret | composition Protein, ≥50% biuret | composition - | composition Protein, 5.0-20.0% |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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