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Anti-GroEL antibody produced in rabbit

Name: Anti-GroEL antibody produced in rabbit
Brand: SIGMA
Price: 635 USD
Availability: InStock

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-GroEL, GroEL Antibody - Anti-GroEL antibody produced in rabbit - Tested Applications: immunoblotting 1:80,000,indirect ELISA 1:40,000-1:80,000 - Sigma Aldrich G6532, Groel Antibody

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Size/SKU	Availability	Price
0.5 mL	Available to ship TODAYfromMILWAUKEE	$635.00

About This Item

NACRES:

NA.46

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

polyclonal

Application:

ELISA (i), WB

Citations:

$635.00

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

E. coli

technique(s)

indirect ELISA: 1:40,000-1:80,000, western blot: 1:80,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

Escherichia coli ... groEL(913705), groEL(913705)
Escherichia coli K12 ... Grol(948665)

Description

General description

GroEL protein belongs to the folding chaperonin family. It is a 58.3 kDa protein with apical, intermediate and equatorial functional domains.

Immunogen

purified recombinant GroEL produced in E. coli.

Application

Anti-GroEL antibody produced in rabbit has been used as positive control in histology and immunohistochemistry of Dirofilaria immitis, nematode worms and in the western blot analysis of the HeLa cells.

Biochem/physiol Actions

GroEL, together with GroES, in the presence of ATP, assists in protein folding. Co-expression of aldehyde dehydrogenase with GroEL/GroES promotes folding and higher production of protein in soluble form. GroES and GroEL bind to the protein substrate, enclosing it in a cage like fold. The target protein after folding gets dissociated from the cage and is accompanied with hydrolysis of adenosine triphosphate (ATP) to adenosine diphosphate (ADP).

The antibody does not cross-react with GroES and bovine HSP 70.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	G7781	S6390	G2781
$Anti-GroEL antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich G6532 Anti-GroEL antibody produced in rabbit	$Anti-Glutathione-S-Transferase (GST) antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich G7781 Anti-Glutathione-S-Transferase (GST) antibody produced in rabbit	Sigma-Aldrich S6390 Anti-Streptavidin antibody produced in rabbit	$Anti-Glutamine Synthetase antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich G2781 Anti-Glutamine Synthetase antibody produced in rabbit
description IgG fraction of antiserum, buffered aqueous solution	description IgG fraction of antiserum, buffered aqueous solution	description whole antiserum	description IgG fraction of antiserum, buffered aqueous solution
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
antibody form IgG fraction of antiserum	antibody form IgG fraction of antiserum	antibody form whole antiserum	antibody form IgG fraction of antiserum
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
species reactivity E. coli	species reactivity -	species reactivity -	species reactivity rat
technique(s) indirect ELISA: 1:40,000-1:80,000, western blot: 1:80,000	technique(s) dot blot: suitable, indirect ELISA: 1:10,000, western blot: 1:2,000 using lysates of induced Escherichia coli expressing GST	technique(s) immunoelectrophoresis: suitable, indirect ELISA: 1:10,000	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:10,000 using rat brain sections, microarray: suitable, western blot: 1:10,000 using rat brain cytosolic fraction

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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