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G6751

α-Glycerophosphate Dehydrogenase from rabbit muscle

Type I, ammonium sulfate suspension, 100-300 units/mg protein

Synonym(s):

sn-Glycerol-3-phosphate Dehydrogenase from rabbit muscle, sn-Glycerol-3-phosphate:NAD+ 2-oxidoreductase

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Size/SKUAvailabilityPrice
200 units
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$79.10
1000 units
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$147.00
5000 units
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$508.00
10000 units
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$817.00

About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-982-3
MDL number:
EC Number:
Specific activity:
100-300 units/mg protein
Biological source:
rabbit muscle

$79.10


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biological source

rabbit muscle

Quality Segment

type

Type I

form

ammonium sulfate suspension

specific activity

100-300 units/mg protein

storage condition

(Tightly closed)

technique(s)

activity assay: suitable

color

white

foreign activity

Lactic dehydrogenase, pyruvate kinase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase ≤0.01%, Triosephosphate isomerase ≤0.02%

storage temp.

2-8°C

General description

α-Glycerophosphate dehydrogenase is a membrane protein and a flavin-linked primary dehydrogenase.

Research area: Cell Signaling

Application

α-Glycerophosphate Dehydrogenase from rabbit muscle has been used:
  • in the reaction mixture to measure the glycerol kinase activity
  • to demonstrate compartmentalized enzymatic reactions where NADH is involved
  • in the reaction mixture assay for L-fuculose-1-phosphate aldolase

Biochem/physiol Actions

α-Glycerophosphate dehydrogenase catalyzes the oxidation of L-α-glycerophosphate (Glp) to form dihydroxyacetone phosphate (DHAP) and hydrogen peroxide (H2O2). This enzyme is associated with respiratory electron transport chain, glycolysis, and phospholipid metabolism.

Physical form

Crystalline suspension in 3.2 M (NH4)2SO4 and 0.1 g/L EDTA solution, pH 6.0

Preparation Note

Prepared by modification of the method of Fondy.

Analysis Note

Protein determined by biuret.

Other Notes

One unit will convert 1.0 μmole of dihydroxyacetone phosphate to α-glycerophosphate per min at pH 7.4 at 25 °C.

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This Item
G6880P3397G1881
description

Type I, ammonium sulfate suspension, 100-300 units/mg protein

description

Type X, lyophilized powder, ≥100 units/mg protein

description

ammonium sulfate suspension, ≥100 units/mg protein

description

Type III, ammonium sulfate suspension

biological source

rabbit muscle

biological source

-

biological source

rabbit muscle

biological source

rabbit muscle

technique(s)

activity assay: suitable

technique(s)

-

technique(s)

activity assay: suitable

technique(s)

-

specific activity

100-300 units/mg protein

specific activity

≥100 units/mg protein

specific activity

≥100 units/mg protein

specific activity

-

form

ammonium sulfate suspension

form

lyophilized powder

form

ammonium sulfate suspension

form

ammonium sulfate suspension

storage condition

(Tightly closed)

storage condition

-

storage condition

(Tightly closed)

storage condition

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C


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Pictograms

Health hazard

Signal Word

Danger

Hazard Codes

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable



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