The product is cited in the reference Sulaimana, Saba A.J., et al., J of Enz Inhib and Med Chem. 18(4), 297-301 (2003).
G7509
GABase from Pseudomonas fluorescens
lyophilized powder, Protein ≥30 % by biuret
Synonym(s):
GABase Enzyme, GABase from Pseudomonas, Pseudomonas GABase
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| Size/SKU | Availability | Price |
|---|---|---|
10 units | Check Cart for Availability | $589.00 |
25 units | Check Cart for Availability | $1,300.00 |
About This Item
biological source
Pseudomonas fluorescens
Quality Segment
assay
≥30 % (biuret)
form
lyophilized powder
specific activity
≥0.5 units/mg protein
composition
Protein, ≥30% biuret
technique(s)
inhibition assay: suitable
storage temp.
−20°C
General description
Application
Biochem/physiol Actions
GABase catalyzes the conversion of γ-aminobutyric acid (GABA) to succinic acid in the central nervous system. It is used to measure GABA levels in various biological samples. p-Hydroxybenzaldehyde (HBA) is known to be a potential inhibitor on GABase.
Physical form
Partially purified lyophilized powder containing buffer salts and stabilizer
Other Notes
One unit will convert 1.0 μmole of γ-aminobutyric acid (GABA) to succinic semialdehyde and then to succinate per min with a stoichiometric reduction of 1.0 μmole of NADP+ at pH 8.6 at 25 °C.
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This Item | ||
|---|---|---|
| description lyophilized powder, Protein ≥30 % by biuret | description recombinant, expressed in E. coli, ammonium sulfate suspension, ≥50 units/mg protein (biuret) | description lyophilized powder, recombinant, expressed in E. coli |
| assay ≥30 % (biuret) | assay 0.5—2.0 mg protein/mL (biuret) | assay - |
| technique(s) inhibition assay: suitable | technique(s) - | technique(s) - |
| biological source Pseudomonas fluorescens | biological source Pseudomonas fluorescens | biological source - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form ammonium sulfate suspension | form lyophilized powder |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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Are there any literature references that cite use of the Product No. G7509, GABase from Pseudomonas fluorescens?
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How do I solubilize Product No. G7509, GABase from Pseudomonas fluorescens?
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Sigma recommends to dissolve the product with 75 mM phosphate buffer, pH 7.2, containing 25% (v/v) glycerol to a concentration of 2-5 units per mL. Keep the solution in an ice bath while using and store solution frozen. This solution may be stored frozen and thawed several times with only slight loss of activity.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the shelf life of the Product No.G7509, GABase from Pseudomonas fluorescens?
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Sigma recommends retesting the product 3 years after its release date. The retest date will appear on the lot specific Certificate of Analysis for the product.
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