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Granulocyte Colony-Stimulating Factor from mouse

Name: Granulocyte Colony-Stimulating Factor from mouse
Brand: SIGMA
Price: 1030 USD
Availability: InStock

>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym(s):

G-CSF

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Size/SKU	Availability	Price
25 μg	Check Cart for Availability	$1,030.00

About This Item

MDL number:

MFCD00166475

NACRES:

NA.77

UNSPSC Code:

12352202

Biological source:

mouse

Recombinant:

expressed in E. coli

Assay:

>97% (SDS-PAGE)

Form:

lyophilized powder

Mol wt:

19 kDa (179 amino acids including N-terminal methionine)

Impurities:

≤0.01 EU/μg

$1,030.00

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Properties

Product Name

Granulocyte Colony-Stimulating Factor from mouse, G-CSF, recombinant, expressed in E. coli, suitable for cell culture

biological source

mouse

Quality Segment

100

recombinant

expressed in E. coli

assay

>97% (SDS-PAGE)

form

lyophilized powder

potency

10-60 pg/mL ED₅₀

quality

endotoxin tested

mol wt

19 kDa (179 amino acids including N-terminal methionine)

packaging

pkg of 5 and 25 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤0.01 EU/μg

UniProt accession no.

P09920

storage temp.

−20°C

Gene Information

mouse ... Csf3(12985), Csf3r(12986)

Description

General description

The Csf3r (colony stimulating factor 3 receptor (granulocyte)) gene encodes a prototypical mobilizing cytokine.

Application

Granulocyte Colony-Stimulating Factor from mouse has been used in cytokine therapy.

Biochem/physiol Actions

G-CSF is a proliferation, differentiation, survival, and activation factor for hematopoietic cells of the restricted neutrophilic granulocyte lineage. It is produced by macrophages activated by endotoxin (LPS), by monocytes activated by TNF-α with IFNγ, by fibroblasts and endothelial cells activated by IL-1 or TNF-α, and by bone marrow stromal cells activated by IL-1 or LPS. It is suggested that during the inflammatory process endotoxins stimulate tissue macrophages to produce not only G-CSF but several other cytokines, including IL-1 and TNF-α, which in turn stimulate more G-CSF release from endothelial cells and fibroblasts. G-CSF can also synergize with IL-3 to shorten the G₀ period of early hematopoietic progenitors. In addition to the namesake proliferative activity, G-CSF acts on mature neutrophils to enhance their survival and to stimulate their tumorcidal activity. Human G-CSF binds and activates a 130 kD to150 kD glycoprotein single chain receptor that has been classified as a member of the hematopoietic (cytokine) receptor family, the cytokine receptor class I, or the gp130 related cytokine receptor family (although it does not apparently bind to gp130). G-CSF receptors can be found on neutrophils, myeloid leukemia cells that respond to G-CSF, bone marrow cells of neutrophilic granulocyte lineage, and on placental trophoblasts, plus a soluble form may be expressed. Two forms of human G-CSF (177 and 174 amino acids) are synthesized from a single gene by alternative splicing, but murine G-CSF is a single expressed form of 178 amino acids. Human and murine G-CSF share 73% amino acid sequence homology and full cross-reactivity.

Granulocyte colony-stimulating factor (G-CSF) is a lineage-restricted hematopoietic growth factor, stimulating final mitotic divisions and the terminal cellular maturation of the partially differentiated hematopoietic progenitors. G-CSF^3,4 is produced by monocytes and fibroblasts. It stimulates granulocyte colony formation, activates neutrophils and mature granulocytes, and promotes differentiation of certain myeloid leukemic cells. Natural G-CSF is a glycoprotein of 177 amino acids and a molecular weight of 19 kDa.⁵ Human and murine G-CSF have about 75% homology and show biological cross-reactivity.

Physical form

Lyophilized from a 0.2 μm filtered solution in 10 mM citric acid, 0.01% TWEEN^® 20, pH 4.0, containing 50 μg bovine serum albumin per 1 μg of cytokine.

Analysis Note

The biological activity is measured in a cell proliferation assay using a murine myeloblastic cell line, NFS-60.

Legal Information

TWEEN is a registered trademark of Croda International PLC

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This Item	G0282	SRP3050	SRP3331
Sigma-Aldrich G8160 Granulocyte Colony-Stimulating Factor from mouse	Sigma-Aldrich G0282 Granulocyte-Macrophage Colony-Stimulating Factor from mouse	Sigma-Aldrich SRP3050 Granulocyte-Macrophage Colony-Stimulating Factor human	Sigma-Aldrich SRP3331 G-CSF from mouse
description G-CSF, recombinant, expressed in E. coli, suitable for cell culture	description GM-CSF, from mouse, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture	description GM-CSF, Animal-component free, recombinant, expressed in E. coli, suitable for cell culture	description Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)
biological source mouse	biological source mouse	biological source human	biological source mouse
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) -
assay >97% (SDS-PAGE)	assay ≥97% (SDS-PAGE)	assay ≥98% (SDS-PAGE)	assay ≥98% (SDS-PAGE), ≥98% (HPLC)
recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli
Quality Level 100	Quality Level 200	Quality Level 200	Quality Level -
form lyophilized powder	form lyophilized powder	form lyophilized	form lyophilized

Pictograms

GHS07

Signal Word

Warning

Hazard Codes

H319,H335

Precautionary Statements

P261 - P264 - P271 - P280 - P304 + P340 + P312 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)