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G8415

L-Glutamic acid

98.5-100.5%, suitable for cell culture, non-animal source, meets EP testing specifications

Synonym(s):

(S)-2-Aminopentanedioic acid, Glu

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Size/SKUAvailabilityPrice
10 mg

Available to ship TODAYfromMILWAUKEE

$34.20
100 g

Available to ship TODAYfromMILWAUKEE

$52.70
1 kg

Available to ship TODAYfromMILWAUKEE

$206.00

About This Item

Linear Formula:
HO2CCH2CH2CH(NH2)CO2H
CAS Number:
Molecular Weight:
147.13
PubChem Substance ID:
eCl@ss:
32160406
UNSPSC Code:
12352209
NACRES:
NA.26
EC Number:
200-293-7
MDL number:
Beilstein/REAXYS Number:
1723801

$34.20


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Product Name

L-Glutamic acid, from non-animal source, meets EP testing specifications, suitable for cell culture, 98.5-100.5%

biological source

non-animal source

Quality Segment

agency

meets EP testing specifications

assay

98.5-100.5%

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

color

white

mp

205 °C (dec.) (lit.)

solubility

1 M HCl: 100 mg/mL

density

1.54 g/cm3 at 20 °C

anion traces

chloride (Cl-): ≤200 ppm, sulfate (SO42-): ≤200 ppm

cation traces

As: ≤1 ppm, passes test, Fe: ≤10 ppm, passes test, NH4+: ≤200 ppm, passes test

application(s)

advanced drug delivery
pharmaceutical (small molecule)

SMILES string

N[C@@H](CCC(O)=O)C(O)=O

InChI

1S/C5H9NO4/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H,7,8)(H,9,10)/t3-/m0/s1

InChI key

WHUUTDBJXJRKMK-VKHMYHEASA-N

Application

L-Glutamic acid has been used as a supplement in for cell culture media to culture hippocampal neurons and hT-PA (human tissue plasminogen activation) producing cell line.

Biochem/physiol Actions

An excitatory amino acid neurotransmitter that is an agonist at all subtypes of glutamate receptors (metabotropic, kainate, NMDA, and AMPA).
Glutamic acid, or glutamate, the salt form of glutamic acid, functions as a neurotransmitter and also serves as a precursor to other neurotransmitters such as γ-aminobutyric acid. Glutamic acid also plays a key role in many metabolic pathways, that controls growth, reproduction, maintenance and immunity. It is converted to α-ketoglutarate, a key component of the TCA (tricarboxylic acid) cycle, and a precursor for the biosynthesis of nucleic acids and certain amino acids. In cells, glutamine is converted to glutamate by the enzyme glutaminase. Glutamine serves as a source of energy for rapidly dividing cells comprising lymphocytes, enterocytes, macrophages and tumors. Glutamine mediates protein turnover via cellular mTOR (mammalian target of rapamycin) signaling. It is also known to be associated with the inhibition of apoptosis.

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This Item
49449W32850295436
description

from non-animal source, meets EP testing specifications, suitable for cell culture, 98.5-100.5%

description

BioUltra, ≥99.5% (NT)

description

FCC

description

certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

form

powder

form

powder

form

powder or crystals

form

-

assay

98.5-100.5%

assay

≥99.5% (NT)

assay

98.5-101.5% dry basis (perchloric acid titration)

assay

-

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

impurities

endotoxin, tested

impurities

≤0.5% foreign amino acids, insoluble matter, passes filter test

impurities

-

impurities

-

cation traces

As: ≤1 ppm, passes test, Fe: ≤10 ppm, passes test, NH4+: ≤200 ppm, passes test

cation traces

Ni: ≤5 mg/kg, Cr: ≤5 mg/kg, K: ≤50 mg/kg, Ca: ≤200 mg/kg, Mg: ≤5 mg/kg, Sr: ≤5 mg/kg, Cu: ≤5 mg/kg, Li: ≤5 mg/kg, Ba: ≤5 mg/kg, Co: ≤5 mg/kg, Na: ≤200 mg/kg, Zn: ≤5 mg/kg, Mo: ≤5 mg/kg, Bi: ≤5 mg/kg, NH4+: ≤100 mg/kg, Mn: ≤5 mg/kg, Al: ≤5 mg/kg, Pb: ≤5 mg/kg, Cd: ≤5 mg/kg, Fe: ≤5 mg/kg

cation traces

Pb: ≤5 ppm, Hg: ≤1 ppm, As: ≤3 ppm, Cd: ≤1 ppm

cation traces

-

Quality Level

300

Quality Level

200

Quality Level

400, 300

Quality Level

300


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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Questions

1–3 of 3 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. I have to do a solution 147,13mg/ml Can I frooze aliquot of this solution?

    1 answer
    1. Unfortunately, information regarding the solution stability of Product G8415, L-Glutamic acid is not determined. It is recommended to dissolve the product and use freshly prepared dilutions.

      Helpful?

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