G8880
Glutaminase from Escherichia coli
Grade V, lyophilized powder, ≥50 units/mg protein
Synonym(s):
L-Glutamine amidohydrolase
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About This Item
biological source
Escherichia coli
Quality Segment
type
Grade V
form
lyophilized powder
specific activity
≥50 units/mg protein
mol wt
110 kDa
composition
Protein, ~30% biuret
solubility
water: 1 mg/mL (Clear w/Particle to Sl. Hazy w/Particles)
cation traces
NH4+: <0.1 μg/unit
foreign activity
NADH Oxidase ≤0.01%
storage temp.
−20°C
General description
Application
- for the determination of glutamine from the cerebral cortex tissue extract
- for immobilization on aminopropylsilylated controlled pore glass (CPG) for determination of glutamine
- in combination with acivicin in in vitro melanoma cells and MCF-7 and OAW-42 cells for cell proliferation and invasiveness assay.
Biochem/physiol Actions
Physical form
Other Notes
1 of 1
This Item | |||
|---|---|---|---|
| description Grade V, lyophilized powder, ≥50 units/mg protein | description lyophilized powder, 400-2,000 units/mg protein | description lyophilized powder, 30-60 units/mg protein (in glycine buffer) | description recombinant, expressed in E. coli, essentially salt-free, lyophilized powder, ≥3 units/mg protein |
| biological source Escherichia coli | biological source - | biological source - | biological source Escherichia coli |
| specific activity ≥50 units/mg protein | specific activity 400-2,000 units/mg protein | specific activity 30-60 units/mg protein (in glycine buffer) | specific activity ≥3 units/mg protein |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form essentially salt-free, lyophilized powder |
| mol wt 110 kDa | mol wt - | mol wt - | mol wt 11.7 kDa |
| solubility water: 1 mg/mL (Clear w/Particle to Sl. Hazy w/Particles) | solubility H2O: soluble 0.95-1.05 mg/mL, clear to hazy | solubility - | solubility water: 1.90-2.10 mg/mL, clear, colorless |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
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Storage Class
11 - Combustible Solids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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