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G8880

Glutaminase from Escherichia coli

Grade V, lyophilized powder, ≥50 units/mg protein

Synonym(s):

L-Glutamine amidohydrolase

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Size/SKUAvailabilityPrice
10 units
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$192.00
50 units
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$431.00

About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
MDL number:
Specific activity:
≥50 units/mg protein
Biological source:
Escherichia coli

$192.00


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biological source

Escherichia coli

Quality Segment

type

Grade V

form

lyophilized powder

specific activity

≥50 units/mg protein

mol wt

110 kDa

composition

Protein, ~30% biuret

solubility

water: 1 mg/mL (Clear w/Particle to Sl. Hazy w/Particles)

cation traces

NH4+: <0.1 μg/unit

foreign activity

NADH Oxidase ≤0.01%

storage temp.

−20°C

General description

Glutaminase is an amidohydrolase. It has the catalytic domain in the N-terminal and the C-terminal extension possesses STAS domain.

Application

Glutaminase from E.coli has been used:
  • for the determination of glutamine from the cerebral cortex tissue extract
  • for immobilization on aminopropylsilylated controlled pore glass (CPG) for determination of glutamine
  • in combination with acivicin in in vitro melanoma cells and MCF-7 and OAW-42 cells for cell proliferation and invasiveness assay.

Biochem/physiol Actions

Glutaminase from Escherichia coli along with acivicin, a glutamine analog regulates proliferation and invasiveness of cancer cells. Bacterial glutaminase is implicated in increasing the life span of cancer patients by lowering the tumor burden.
Glutaminase catalyzes the conversion of glutamine to glutamate.

Physical form

Lyophilized powder containing potassium succinate and EDTA

Other Notes

One unit will deaminate 1.0 μmole of L-glutamine per min at pH 4.9 at 37 °C.

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This Item
A3809A7437D0690
description

Grade V, lyophilized powder, ≥50 units/mg protein

description

lyophilized powder, 100-300 units/mg protein (biuret)

description

lyophilized powder, ≥150 units/mg protein (biuret)

description

aqueous glycerol solution

biological source

Escherichia coli

biological source

-

biological source

-

biological source

Escherichia coli

specific activity

≥50 units/mg protein

specific activity

100-300 units/mg protein (biuret)

specific activity

≥150 units/mg protein (biuret)

specific activity

-

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

aqueous glycerol solution

solubility

water: 1 mg/mL (Clear w/Particle to Sl. Hazy w/Particles)

solubility

-

solubility

-

solubility

-

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−70°C

mol wt

110 kDa

mol wt

-

mol wt

-

mol wt

~374 kDa


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Storage Class

11 - Combustible Solids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)



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