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H0412

Anti-phospho-Histone H3 (pSer10) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

PH3 Antibody - Anti-phospho-Histone H3 (pSer10) antibody produced in rabbit, Ph3 Antibody, Anti-H3S10p

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Size/SKUAvailabilityPrice
200 μL
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$672.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD01633984
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ARR, IF, WB
Citations:
66

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biological source

rabbit

Quality Segment

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 17 kDa

species reactivity

Caenorhabditis elegans, bovine, human, rat, chicken, Drosophila, frog, mouse

technique(s)

indirect immunofluorescence: 1:500 using human epitheloid carcinoma HeLa cell line treated with nocodazole., microarray: suitable, western blot: 1:2,000 using whole cell extract of human acute T cell leukemia Jurkat cell line treated with nocodazole.

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pSer10)

Gene Information

General description

Monoclonal Anti-Phosphoserine (mouse IgG1 isotype) is derived from the PSR-45 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with phosphoserine conjugated to KLH. Histone proteins H3, H4, H2A, H2B and H1 function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibres. The gene encoding Histone H3 is mapped to human chromosome 1q42.12.
Rabbit polyclonal anti-phospho-Histone H3 (pSer10) antibody recognizes histone H3 phosphorylated on Ser10 (17 kDa). Staining of pSer10-histone H3 in immunoblotting is specifically inhibited with the [pSer10] histone H3 immunizing peptide (human, amino acids 7-20). No inhibition is observed with the respective non-phosphorylated histone H3 peptide histone H3 (human, amino acids 7-20).

Immunogen

synthetic, phosphorylated peptide (pSer10) histone H3 (amino acids 7-20) corresponding to the N-terminus of human histone H3. The sequence is identical in many species.

Application

Anti-phospho-Histone H3 (pSer10) antibody produced in rabbit has been used in the detection of pSer10-histone H3 by immunoblotting, immunohistochemistry and by immunofluorescence.
Rabbit polyclonal anti-phospho-Histone H3 (pSer10) antibody is used to tag Ser10 phosphorylated histone H3 for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques such as immunoblotting and immunofluorescence. It is used as a probe to determine the presence and roles of Ser10 phosphorylated histone H3 in chromosome condensation during both mitosis and meiosis.

Biochem/physiol Actions

Phosphorylation of histone H3, referred to as the nucleosomal response, is localized to a small fraction of highly acetylated H3s and occurs primarily in response to mitogenic and stress stimuli. Histone H3 is phosphorylated during mitosis on at least two serine residues, Ser10 and Ser28. Phosphorylation of Ser10 is considered a crucial event for the onset of mitosis. It is tightly correlated with chromosome condensation during both mitosis and meiosis. Members of the Aurora/AIK family of protein kinases are involved in the mitotic phosphorylation of histone H3 at Ser10 residue. Expression of Aurora-A and Aurora-B is tightly coordinated with histone H3 phosphorylation at G2/M transition and both kinases efficiently phosphorylate H3 at Ser10 in vitro and in vivo. Phosphorylation of H3 at Ser10 is also directly correlated with the induction of immediate-early genes such as c-jun, c-fos and c-myc. Protein kinase A (PKA), ribosomal protein S6 kinase-3 (Rsk-2) and mitogen and stress activated protein kinase-1 (Msk-1) are necessary for the histone H3 phosphorylation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-phospho-Histone H3 (pSer10) antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

Sigma-Aldrich

H0412

Anti-phospho-Histone H3 (pSer10) antibody produced in rabbit

Monoclonal Anti-phospho-Histone H3 (pSer28) antibody produced in rat ~0.5 mg/mL, clone HTA28, purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

H9908

Monoclonal Anti-phospho-Histone H3 (pSer28) antibody produced in rat

Anti-Histone H3 antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

Sigma-Aldrich

H0164

Anti-Histone H3 antibody produced in rabbit

Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit 2-2.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

H9286

Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit

description

IgG fraction of antiserum, buffered aqueous solution

description

~0.5 mg/mL, clone HTA28, purified immunoglobulin, buffered aqueous solution

description

IgG fraction of antiserum, buffered aqueous solution

description

2-2.5 mg/mL, affinity isolated antibody, buffered aqueous solution

biological source

rabbit

biological source

rat

biological source

rabbit

biological source

rabbit

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody form

purified immunoglobulin

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

clone

polyclonal

clone

HTA28, monoclonal

clone

polyclonal

clone

polyclonal

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Storage Class

12 - Non Combustible Liquids

WGK

nwg

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