Key Documents

Anti-acetyl-Histone H3 (Ac-Lys⁹) antibody, Mouse monoclonal

Name: Anti-acetyl-Histone H3 (Ac-Lys<SUP>9</SUP>) antibody, Mouse monoclonal
Brand: SIGMA
Price: 192 USD
Availability: InStock

clone AH3-120, purified from hybridoma cell culture

Synonym(s):

Anti-H3K9ac

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Size/SKU	Availability	Price
25 μL	Check Cart for Availability	$192.00
200 μL	Check Cart for Availability	$789.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD02685921

Conjugate:

unconjugated

Clone:

AH3-120, monoclonal

Application:

ARR, ELISA (i), ICC, WB

Citations:

$192.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

AH3-120, monoclonal

form

buffered aqueous solution

mol wt

antigen ~17 kDa

species reactivity

human, bovine, Caenorhabditis elegans, frog, Drosophila, chicken, rat, mouse

packaging

antibody small pack of 25 μL

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable, indirect ELISA: suitable, microarray: suitable, western blot: 1-2 μg/mL using whole cell extract of mouse fibroblasts 3T3 cell line treated with sodium butyrate

isotype

IgG1

UniProt accession no.

P68431

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

acetylation (Lys9)

Description

General description

Monoclonal Anti-Acetyl-Histone H3 (Ac-Lys9) recognizes human histone H3 when acetylated on Lys9. Staining of the histone H3-Ac-Lys9 band in immunoblotting is specifically inhibited with the acetylated histone H3 immunizing peptide but not with the nonacetylated one.

Immunogen

synthetic, acetylated histone H3 peptide (amino acids 7-20, Ac-Lys⁹) corresponding to the N-terminus of human histone H3. The sequence is identical in many species including mouse, rat, bovine, chicken, frog, Drosophila, and C. elegans, and is highly conserved (single amino acid substitution) in Tetrahymena histone H3.

Application

Monoclonal Anti-acetyl-Histone H3 (Ac-Lys9) antibody may be used in various applications including ELISA, immunoblotting (approx. 17 kDa), and immunocytochemistry.

Biochem/physiol Actions

Acetyl-Histone H3 hav Acetylation of lysine residues within these N-terminal domains of histones by histone acetyl-transferase (HATs), including Gcn5p, PCAF, p300/CBP and TAFII250 is associated with transcriptional activation. This modification results in remodeling of the nucleosome structure into an open conformation more accessible to transcription complexes. Conversely, histone deacetylation by histone deacetylase (HDACs) is associated with transcription repression reversing the chromatin remodeling process. In most species, histone H3 is primarily acetylated at lysine 9, 14, 18, and 23. Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Acetylation of specific lysines in histone H3 is also associated with processes apart from transcription. During DNA replication, new histones are rapidly synthesized and assembled into replicated DNA. Histones H3 and H4 are brought to replicating chromatin in a pre-acetylated state that turns into a de-acetylated state after replication is completed and the newly assembled chromatin matures.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	H9286	H0412	SAB4200651
Sigma-Aldrich H0913 Anti-acetyl-Histone H3 (Ac-Lys⁹) antibody, Mouse monoclonal	Sigma-Aldrich H9286 Anti-acetyl-Histone H3 (Ac-Lys⁹) antibody produced in rabbit	$Anti-phospho-Histone H3 (pSer10) antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich H0412 Anti-phospho-Histone H3 (pSer¹⁰) antibody produced in rabbit	Sigma-Aldrich SAB4200651 Monoclonal Anti-Histone H3 (C-terminal) antibody produced in mouse
description clone AH3-120, purified from hybridoma cell culture	description 2-2.5 mg/mL, affinity isolated antibody, buffered aqueous solution	description IgG fraction of antiserum, buffered aqueous solution	description clone H6.10, purified from hybridoma cell culture
antibody form purified immunoglobulin	antibody form affinity isolated antibody	antibody form IgG fraction of antiserum	antibody form purified immunoglobulin
clone AH3-120, monoclonal	clone polyclonal	clone polyclonal	clone H6.10, monoclonal
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate -
Gene Information human ... H3F3A(3020), H3F3B(3021), HIST1H3A(8350), HIST1H3B(8358), HIST1H3C(8352), HIST1H3D(8351), HIST1H3E(8353), HIST1H3F(8968), HIST1H3G(8355), HIST1H3H(8357), HIST1H3I(8354), HIST1H3J(8356), HIST2H3A(333932), HIST2H3C(126961), HIST3H3(8290) mouse ... H3f3a(15078), H3f3b(15081), Hist1h3a(360198), Hist1h3b(319150), Hist1h3c(319148), Hist1h3d(319149), Hist1h3e(319151), Hist1h3f(260423), Hist1h3g(97908), Hist1h3h(319152), Hist1h3i(319153), Hist2h3b(319154), Hist2h3c1(15077), Hist2h3c2(97114) rat ... H3f3b(117056)	Gene Information human ... H3F3A(3020), H3F3B(3021), HIST1H3A(8350), HIST1H3B(8358), HIST1H3C(8352), HIST1H3D(8351), HIST1H3E(8353), HIST1H3F(8968), HIST1H3G(8355), HIST1H3H(8357), HIST1H3I(8354), HIST1H3J(8356), HIST2H3A(333932), HIST2H3C(126961), HIST3H3(8290) mouse ... H3f3a(15078), H3f3b(15081), Hist1h3a(360198), Hist1h3b(319150), Hist1h3c(319148), Hist1h3d(319149), Hist1h3e(319151), Hist1h3f(260423), Hist1h3g(97908), Hist1h3h(319152), Hist1h3i(319153), Hist2h3b(319154), Hist2h3c1(15077), Hist2h3c2(97114) rat ... H3f3b(117056)	Gene Information human ... H3F3A(3020), H3F3B(3021), HIST1H3A(8350), HIST1H3B(8358), HIST1H3C(8352), HIST1H3D(8351), HIST1H3E(8353), HIST1H3F(8968), HIST1H3G(8355), HIST1H3H(8357), HIST1H3I(8354), HIST1H3J(8356), HIST2H3A(333932), HIST2H3C(126961), HIST3H3(8290) mouse ... H3f3a(15078), H3f3b(15081), Hist1h3a(360198), Hist1h3b(319150), Hist1h3c(319148), Hist1h3d(319149), Hist1h3e(319151), Hist1h3f(260423), Hist1h3g(97908), Hist1h3h(319152), Hist1h3i(319153), Hist2h3b(319154), Hist2h3c1(15077), Hist2h3c2(97114) rat ... H3f3b(117056)	Gene Information human ... HIST1H3A(8350)
biological source mouse	biological source rabbit	biological source rabbit	biological source mouse
species reactivity human, bovine, Caenorhabditis elegans, frog, Drosophila, chicken, rat, mouse	species reactivity bovine, human, Caenorhabditis elegans, frog, mouse, rat, Drosophila, chicken	species reactivity Caenorhabditis elegans, bovine, human, rat, chicken, Drosophila, frog, mouse	species reactivity chicken, human, monkey, hamster, canine

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