Product H1029, Monoclonal Anti-polyHistidine antibody requires at least 6 histidines in a row for recognition.
H1029
Monoclonal Anti-polyHistidine antibody produced in mouse
clone HIS-1, ascites fluid
Synonym(s):
Monoclonal Anti-polyHistidine, Monoclonal 6 His epitope tag, Monoclonal 6xHis-tag, Monoclonal HHHHHH epitope tag, Monoclonal Hexa His tag, Monoclonal His-tag, Monoclonal His6 tag, Monoclonal Histidine tagged, Monoclonal Poly-His-tag
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| Size/SKU | Availability | Price |
|---|---|---|
100 μL | Available to ship TODAYfromMILWAUKEE | $577.00 |
0.2 mL | Available to ship TODAYfromMILWAUKEE | $785.00 |
About This Item
NACRES:
NA.56
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
HIS-1, monoclonal
Application:
DB, ELISA (i), IP, WB
Citations:
448
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
HIS-1, monoclonal
form
liquid
technique(s)
dot blot: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 1:3,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein
isotype
IgG2a
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
The Monoclonal Anti-polyHistidine (mouse IgG2a) antibody recognizes native or denatured, reduced forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins. The antibody preferentially recognizes the N-terminal tagged fusion protein and is reactive with fusion proteins expressed by the prokaryotic expression vectors pET, pRSET, and pTrc.
Immunogen
recombinant polyHistidine tagged fusion protein.
Application
Antibody suitable for immunoblotting, dot blot assays, ELISA and immunocytochemistry. Monoclonal Anti-polyHistidine antibody produced in mouse has been used in Western blotting.
Biochem/physiol Actions
The IgG2a (Immunoglobulin G 2a) gene actively takes part in stimulating autoimmunity in mice. The gene is found to be highly expressed in virus infection.
The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.
Physical form
Suppled as ascites fluid with 15mM sodium azide.
Other Notes
Recommended dilution for immunoblotting is 1:3000
Legal Information
This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.
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This Item | |||
|---|---|---|---|
| description clone HIS-1, ascites fluid | description clone HIS-1, purified immunoglobulin | description clone HIS-1, purified from hybridoma cell culture | description clone HIS-1, purified from hybridoma cell culture |
| antibody form ascites fluid | antibody form purified immunoglobulin | antibody form purified from hybridoma cell culture | antibody form purified from hybridoma cell culture |
| clone HIS-1, monoclonal | clone HIS-1, monoclonal | clone HIS-1, monoclonal | clone HIS-1, monoclonal |
| conjugate unconjugated | conjugate - | conjugate peroxidase conjugate | conjugate alkaline phosphatase conjugate |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| technique(s) indirect ELISA: suitable, immunoprecipitation (IP): suitable, dot blot: suitable, western blot: 1:3,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein | technique(s) immunoblotting: 0.25-0.5 μg/mL, immunofluorescence: 4-8 μg/mL, flow cytometry: 3-6 μg/test | technique(s) western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein | technique(s) western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein |
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Storage Class
10 - Combustible liquids
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How many histidines are required for proper recognition by Product H1029, Monoclonal Anti-polyHistidine antibody?
1 answer-
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Is Product H1029, Monoclonal Anti-polyHistidine antibody, suitable for use in immunofluorescence studies?
1 answer-
Product H1029 has been used in immunofluorescence and a good picture of the staining has been published. Ker R. Marshall, et al., Journal of General Virology 83, 1601-1612 (2002).
Helpful?
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What is the detection limit of Product H1029, Monoclonal Anti-polyHistidine antibody?
1 answer-
The detection limit of product H1029 is 0.5 μg/ml or less.
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