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Monoclonal Anti-Heat Shock Protein 60 antibody produced in mouse

Name: Monoclonal Anti-Heat Shock Protein 60 antibody produced in mouse
Brand: SIGMA
Price: 624 USD
Availability: InStock

clone LK2, ascites fluid

Synonym(s):

Anti-CPN60, Anti-GROEL, Anti-HLD4, Anti-HSP-60, Anti-HSP60, Anti-HSP65, Anti-HuCHA60, Anti-SPG13

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Size/SKU	Availability	Price
0.2 mL	Check Cart for Availability	$624.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD02189138

eCl@ss:

32160702

Conjugate:

unconjugated

Clone:

LK2, monoclonal

Application:

ELISA (i), EM, IHC (p), WB

Citations:

$624.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

LK2, monoclonal

mol wt

antigen 60 kDa

contains

15 mM sodium azide

species reactivity

spinach, chicken, E. coli, helminth, rat, human

technique(s)

electron microscopy: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, indirect ELISA: suitable, western blot: 1:100 using cultured human foreskin fibroblast extract

isotype

IgG1

UniProt accession no.

P10809

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... HSPD1(3329)
rat ... Hspd1(63868)

Description

General description

Monoclonal Anti-Heat Shock Protein 60 (HSP60), clone LK2, recognizes an epitope located between amino acid residues 383-419 of the human (corresponding to a.a. residues 356-393 of the mycobacterial) HSP60. The antibody is reactive against the mammalian (e.g., human, rat), avian (e.g., chicken), bacterial (e.g., E. coli), helminths and spinach HSP60. In immunoblotting, the antibody may label additional bands at approx. 38 and 97 kDa. It is reactive with both the constitutive and the inducible HSP60. It shows a raised level of staining in immunohistochemistry of formalin-fixed, paraffin-embedded synovial membranes taken from patients with juvenile chronic arthritis.

Monoclonal anti-Heat Shock Protein 60 (mouse IgG1 isotype) is derived from the LK2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant human heat shock protein 60.

Immunogen

recombinant human heat shock protein 60 (HSP60).

Application

Monoclonal Anti-Heat Shock Protein 60 antibody produced in mouse has been used in:western blotting, immunocytochemistry, enzyme linked immuno sorbent assay (ELISA) ,immunoblotting

Biochem/physiol Actions

A wide variety of environmental perturbations, such as a sudden increase in temperature, induce cells to rapidly synthesize a group of polypeptides known as the heat shock (stress) proteins. The 60 kDa HSP family (HSP60) which retained a uniquely high level of sequence conservation during evolution is a focus of interest as a potential antigen in a number of autoimmune diseases. Abnormal immune reactivity involving HSP60 has also been implicated in the pathogenesis of schizophrenia. In human arthritis and experimentally-induced arthritis in animals, disease development was seen to coincide with development of immune reactivity directed against not only bacterial HSP60, but also against its mammalian homologue. A human mitochondrial protein, originally designated P1, has been described as the human homologue of the bacterial HSP60, and >45% of the protein has sequence identity with its bacterial homologue (groEL, HSP65).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	SAB5700661	PLA0269	SAB4501464
Sigma-Aldrich H3524 Monoclonal Anti-Heat Shock Protein 60 antibody produced in mouse	Sigma-Aldrich SAB5700661 Anti-Hsp60 antibody produced in rabbit	Sigma-Aldrich PLA0269 Rabbit anti-HSP60 Antibody, Affinity Purified	Sigma-Aldrich SAB4501464 Anti-HSP60 antibody produced in rabbit
description clone LK2, ascites fluid	description -	description Powered by Bethyl Laboratories, Inc.	description affinity isolated antibody
biological source mouse	biological source rabbit	biological source rabbit	biological source rabbit
species reactivity spinach, chicken, E. coli, helminth, rat, human	species reactivity human, rat, mouse	species reactivity human, mouse	species reactivity human, rat, mouse
clone LK2, monoclonal	clone polyclonal	clone polyclonal	clone polyclonal
Gene Information human ... HSPD1(3329) rat ... Hspd1(63868)	Gene Information human ... HSPD1(3329)	Gene Information rabbit ... HSP60(3329)	Gene Information human ... HSPD1(3329)
antibody form ascites fluid	antibody form affinity isolated antibody	antibody form affinity purified immunoglobulin	antibody form affinity isolated antibody
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated

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Storage Class

10 - Combustible liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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