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H4502

Hexokinase from Saccharomyces cerevisiae

Type F-300, lyophilized powder, ≥130 units/mg protein (biuret)

Synonym(s):

Hexokinase D, Hexokinasetype IV, ATP:D-Hexose-6-phosphotransferase, Hexokinase from yeast

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500 units

Estimated to ship onMay 04, 2026fromMILWAUKEE

$88.10
1000 units

Estimated to ship onMay 04, 2026fromMILWAUKEE

$106.00
2500 units

Estimated to ship onMay 04, 2026fromMILWAUKEE

$178.00
10000 units

Estimated to ship onApril 07, 2027fromMILWAUKEE

$531.00

About This Item

CAS Number:
9001-51-8
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-611-5
NACRES:
NA.54
MDL number:
MFCD00131321
EC Number:
2.7.1.1 (BRENDA, IUBMB)
Specific activity:
≥130 units/mg protein (biuret)
Biological source:
Saccharomyces cerevisiae

$88.10

Estimated to ship onMay 04, 2026Details

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biological source

Saccharomyces cerevisiae

Quality Level

200

type

Type F-300

form

lyophilized powder

specific activity

≥130 units/mg protein (biuret)

mol wt

110 kDa

purified by

crystallization

storage condition

(Keep container tightly closed in a dry and well-ventilated place)

color

white

anion traces

sulfate (SO42-): free

UniProt accession no.

P04806, P04807

foreign activity

ATPase, myokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconic dehydrogenase and phosphoglucose isomerase ≤0.01%

storage temp.

−20°C

Gene Information

bakers yeast ... HXK1(850614), HXK2(852639)

General description

Research area: Cell Signaling

The hexokinase 1 (HXK1) gene is mapped to the Saccharomyces cerevisiae chromosome VI.[1] HXK1 is ubiquitously expressed in mammalian tissues and is abundant in the brain, erythrocytes, lymphocytes, and fibroblasts.[2]

Application

Hexokinase has been used:
  • for digesting glucose for measuring glucose content in the root and stem samples of Quercus velutina[3]
  • to treat ADP (adenosine diphosphate) solution along with D-glucose to remove the contaminating ATP (adenosine triphosphate)
  • in 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffer (pH 7.5) for ADP-[13CU]glucose synthesis

Biochem/physiol Actions

Catalyzes the phosphorylation of D-hexose sugars at the C6 position utilizing ATP as a phosphate source.

The rate of phosphorylation varies with different hexoses (pH 7.5, 30 °C).
D-fructose KM: 0.33 mM
D-glucose KM: 0.12 mM
D-mannose KM: 0.05 mM

Yeast hexokinase exists as two similar isoforms, PI and PII (A and B), with isoelectric points of 5.25 and 4, respectively.

Molecular Weight: ~ 54 kDa (monomer)
~110 kDa (dimer)
Optimal pH: 7.5 to 9.0
Extinction Coefficient: E1% = 8.85 (PI) and 9.47 (PII) at 280 nm

Activators: Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds.

Inhibitors: sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg2+ and 4-chloromercuribenzoate)
Hexokinase catalyzes the rate-limiting first step of glycolysis, which involves the phosphorylation of glucose.[4]

Physical form

Lyophilized powder containing approx. 15% sodium citrate

Preparation Note

Reconstitute with water or citrate buffer, pH 7.0. Studies have shown excellent stability during repeated freezing and thawing over a period of at least 30 days.

Analysis Note

A mixture of isoenzymes.

Other Notes

One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25 °C.

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H6380SRP6065A7011
Hexokinase from Saccharomyces cerevisiae Type F-300, lyophilized powder, ≥130 units/mg protein (biuret)

Sigma-Aldrich

H4502

Hexokinase from Saccharomyces cerevisiae

Hexokinase from Saccharomyces cerevisiae lyophilized powder, ≥350 units/mg protein, Protein ≥10 % by biuret

Sigma-Aldrich

H6380

Hexokinase from Saccharomyces cerevisiae

Hexokinase 1 human recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Sigma-Aldrich

SRP6065

Hexokinase 1 human

Alcohol Dehydrogenase from Saccharomyces cerevisiae ≥300 units/mg protein, lyophilized powder (contains buffer salts), Mw 141-151 kDa

Sigma-Aldrich

A7011

Alcohol Dehydrogenase from Saccharomyces cerevisiae

specific activity

≥130 units/mg protein (biuret)

specific activity

≥350 units/mg protein

specific activity

-

specific activity

≥300 units/mg protein

biological source

Saccharomyces cerevisiae

biological source

Saccharomyces cerevisiae

biological source

human

biological source

Saccharomyces cerevisiae

Gene Information

bakers yeast ... HXK1(850614), HXK2(852639)

Gene Information

bakers yeast ... HXK1(850614), HXK2(852639)

Gene Information

human ... HXK1(3098)

Gene Information

-

form

lyophilized powder

form

lyophilized powder

form

liquid

form

lyophilized powder (contains buffer salts)

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−70°C

storage temp.

−20°C

UniProt accession no.

P04806, P04807

UniProt accession no.

P04806, P04807

UniProt accession no.

P19367

UniProt accession no.

A0A3G3NDH9, S5RCH3, S5RK20, S5S176

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000439959
0000439959
0000439958
0000439957
0000439958

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Protocols

Enzymatic Assay of Hexokinase

Measure hexokinase activity using a continuous spectrophotometric rate-determination assay at 340 nm, catalyzing D-hexose sugar phosphorylation using ATP.

Daiki Kubota et al.
Ophthalmic genetics, 41(6), 629-638 (2020-08-21)
The hexokinase 1 (HK1) gene encodes one of the four human hexokinases that play essential roles in glucose metabolism. Recently, several cases of E847K mutation in the HK1 gene were reported to cause inherited retinal dystrophy. The purpose of this
Erin Wiley et al.
Tree physiology, 33(11), 1216-1228 (2013-11-26)
There is no consensus about how stresses such as low water availability and temperature limit tree growth. Sink limitation to growth and survival is often inferred if a given stress does not cause non-structural carbohydrate (NSC) concentrations or levels to
Manfred Schmid et al.
Molecular cell, 21(3), 379-391 (2006-02-04)
Our previous work identified the inner basket of the NPC as a physical activation/protection station for force-tethered, epigenetically silenced genes. Here we show that a specific nucleopore-to-gene-promoter interaction (Nup-PI) is an early physiological event of gene activation. Nup-PI was discovered
View All Related Papers

Global Trade Item Number

SKUGTIN
H4502-500UN04061833795422
H4502-2.5KU04061833795385
H4502-1KU04061833795347
H4502-10KU04061833795316

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