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Anti-HMGB1 (HMG1) (N-terminal) antibody produced in rabbit

Name: Anti-HMGB1 (HMG1) (N-terminal) antibody produced in rabbit
Brand: SIGMA
Price: 501.5 USD
Availability: InStock

Enhanced Validation

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Amphoterin, Anti-High mobility group box 1, Anti-High mobility group protein 1

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Size/SKU	Availability	Price
200 μL	Available to ship TODAYfromMILWAUKEE	$590.00 $501.50

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD02263062

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IF, IP, WB

Citations:

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 25 kDa

species reactivity

human, rat, mouse

enhanced validation

independent
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technique(s)

immunoprecipitation (IP): 10 μg using HEK-293T cell lysates, indirect immunofluorescence: 1-2 μg/mL using paraformaldehyde-Triton fixed PC12 cultured cells., indirect immunofluorescence: suitable, western blot: 1-2 μg/mL using 3T3 cell lysates

UniProt accession no.

P09429

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... HMGB1(3146)
mouse ... Hmgb1(15289)
rat ... Hmgb1(25459)

Description

General description

Anti-HMGB1 (HMG1) (N-terminal) is produced in rabbit using as immunogen a synthetic peptide corresponding to human HMGB1 conjugated to KLH. High Mobility Group B (HMGB) protein family includes HMGB1, HMGB2 and HMGB3, that are highly conserved and indistinguishable in their biochemical properties. HMGB1 is a 25 kDa protein of 215 amino acids and consists of two homologous HMG-boxes rich in basic amino acids, and an acidic tail at the carboxy-terminus.

HMGB proteins belong to the High Mobility Group (HMG) family of proteins that contain the HMG-box for binding and changing DNA structures .

Immunogen

synthetic peptide corresponding to amino acids 2-17 of human HMGB1, conjugated to KLH. The corresponding sequence is conserved in mouse and rat.

Application

Anti-HMGB1 (HMG1) (N-terminal) antibody is suitable for use in chemiluminescent immunoblot (using mouse heart homogenates) . The antibody can also be used in immunoprecipitation (10 μg using HEK-293T cell lysates), indirect immunofluorescence (1-2 μg/mL using paraformaldehyde-Triton fixed PC12 cultured cells), and western blot (1-2 μg/mL using 3T3 cell lysates).

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

Biochem/physiol Actions

High Mobility Group B (HMGB-1 and 2) participate in the regulation of chromatin structure as well as being involved in transcription regulation, DNA repair, recombination, differentiation and extracellular signaling. It shows a defect in transcriptional enhancement of the glucocorticoid receptor, that indicates the important role for HMGB1 in proper transcriptional control by specific transcription factors. Increased expression of HMGB1 is observed in cancer cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	H9539	WH0003146M8	HPA003506
Sigma-Aldrich H9664 Anti-HMGB1 (HMG1) (N-terminal) antibody produced in rabbit	Sigma-Aldrich H9539 Anti-HMGB1 (HMG1) (C-terminal) antibody produced in rabbit	Sigma-Aldrich WH0003146M8 Monoclonal Anti-HMGB1 antibody produced in mouse	Sigma-Aldrich HPA003506 Anti-HMGB2 antibody produced in rabbit
description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution	description clone 2F6, purified immunoglobulin, buffered aqueous solution	description Prestige Antibodies^® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
biological source rabbit	biological source rabbit	biological source mouse	biological source rabbit
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form purified immunoglobulin	antibody form affinity isolated antibody
Quality Level 200	Quality Level 200	Quality Level 100	Quality Level 100
Gene Information human ... HMGB1(3146) mouse ... Hmgb1(15289) rat ... Hmgb1(25459)	Gene Information human ... HMGB1(3146) mouse ... Hmgb1(15289) rat ... Hmgb1(25459)	Gene Information human ... HMGB1(3146)	Gene Information human ... HMGB2(3148)
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

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