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I2276

Interleukin-12 human

≥97% (SDS-PAGE and N-terminal analysis), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

Synonym(s):

IL-12

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5 μg
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$649.00

About This Item

UNSPSC Code:
12352202
NACRES:
NA.32
MDL number:
MFCD00212975

$649.00

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biological source

human

Quality Segment

200

recombinant

expressed in baculovirus infected Sf21 cells

assay

≥97% (SDS-PAGE and N-terminal analysis)

form

lyophilized powder

potency

0.01-0.05 ng/mL ED50

mol wt

glycoprotein 75 kDa

packaging

pkg of 5 μg

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

P29459,
P29460

storage temp.

−20°C

Gene Information

human ... IL12A(3592), IL12B(3593)

General description

Recombinant human Interleukin‑12 (IL‑12) is expressed in baculovirus‑infected Sf21 insect cells and supplied as a lyophilized glycoprotein. The biological activity of recombinant human IL‑12 was measured by its ability to stimulate the proliferation of PHA‑activated human T lymphoblasts. IL‑12 has anti‑angiogenic properties.

Application

Recombinant human IL‑12 has been used in mammalian cell culture research applications, including studies of T‑cell and natural killer (NK) cell activation and cytokine‑mediated signaling pathways in vitro.

Biochem/physiol Actions

Interleukin‑12 (IL‑12) is an immunomodulatory cytokine that mediates cellular responses through activation of T cells and natural killer (NK) cells to produce IFN‑γ. Recombinant human IL‑12 has been widely studied in mammalian cell culture models investigating immune‑related signaling and cytokine‑driven cellular activation pathways.
Interleukin-12 (IL-12) was identified as a factor secreted by human Epstein-Barr (EBV)-transformed B cell lines. IL-12, or Natural Killer Cell Stimulatory Factor (NKSF), is a 75 kDa disulfide-linked heterodimer of a 35 kDa subunit and 40 kDa subunit. IL-12 is produced predominantly by monocytes and NK cells and induces T cells and NK cells to produce IFN-γ. Human IL-12 is not active on mouse cells, but murine IL-12 is active on both murine and human lymphocytes. The biological activity of recombinant human IL-12 was measured by its ability to stimulate the proliferation of PHA-activated human T lymphoblasts. IL-12 has anti-angiogenic properties.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.25 mg bovine serum albumin.

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description

≥97% (SDS-PAGE and N-terminal analysis), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

description

HumanKine®, recombinant, expressed in HEK 293 cells, suitable for cell culture, endotoxin, tested

description

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

description

≥97% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in HEK 293 cells

recombinant

expressed in E. coli

recombinant

expressed in baculovirus infected Sf21 cells

biological source

human

biological source

human

biological source

human

biological source

human

assay

≥97% (SDS-PAGE and N-terminal analysis)

assay

≥95% (SDS-PAGE)

assay

≥97% (SDS-PAGE)

assay

≥97% (SDS-PAGE)

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

lyophilized powder

form

lyophilized

form

lyophilized powder

form

lyophilized powder

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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