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I2519

Monoclonal Anti-Interleukin-8 antibody produced in mouse

clone 6217, purified immunoglobulin, lyophilized powder

Synonym(s):

Anti-CXCL8, Anti-IL-8

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Size/SKUAvailabilityPrice
0.5 mg

Available to ship TODAYfromMILWAUKEE

$1,130.00
$960.50

About This Item

UNSPSC Code:
51111800
NACRES:
NA.41
MDL number:
MFCD00212220
Conjugate:
unconjugated
Clone:
6217, monoclonal
Application:
ELISA (c), FACS, ICC, Neutral, WB
Citations:
8

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biological source

mouse

Quality Segment

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6217, monoclonal

form

lyophilized powder

species reactivity

human

technique(s)

capture ELISA: suitable, flow cytometry: suitable, immunocytochemistry: 25 μg/mL using using calcium ionophore- and PMA-stimulated human peripheral blood mononuclear cells, neutralization: suitable, western blot: 1-2 μg/mL

isotype

IgG1

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... IL8(3576)

General description

Interleukin-8 or CXCL8 (IL-8) is a proinflammatory chemokine that belongs to the C-X-C family. Monocytes and macrophages are the primary sources of IL-8 but it is also secreted by most nucleated cells. IL-8 binds to two G protein-coupled receptors, CXCR1 and CXCR2 and leads to the downstream activation of PI3K, MAPK, PKC, STAT3 and FAK pathways. IL-8 is responsible for the recruitment monocytes and neutrophils that results in a prolonged acute inflammation. The expression of IL-8 is influenced by inflammatory signals, chemical and environmental stress and hormones. Deregulation of IL-8 signaling has been observed in atherosclerosis, cardiovascular disease and solid tumors of bladder, prostate, ovary, glial cells and pancreas. Monoclonal Anti-Interleukin-8 antibody recognizes human IL-8. By immunoblotting, the antibody shows 100% cross reactivity with recombinant porcine IL-8, and no cross reactivity with recombinant rat CINC-2α.

Immunogen

purified, E. coli-derived, recombinant human IL-8.

Application

Monoclonal Anti-Interleukin-8 antibody may be used for neutralization assays; ND50 is 0.08-0.4 μg/ml. The antibody may be used for immunoblotting at a working concentration of 1-2 μg/ml. For ELISA a working concentration of 0.5 μg/ml may be used. Antibody concentration of 25 μg/ml may be used for immunocytochemistry using stimulated human peripheral blood mononuclear cells. For flow cytometry, 10 μL of 25 μg/ml stock antibody solution may be used for 1-5 X 105 cells.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Monoclonal Anti-Interleukin-8 antibody produced in mouse clone 6217, purified immunoglobulin, lyophilized powder

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Monoclonal Anti-IL8 antibody produced in mouse clone 4E3, purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

SAB1409246

Monoclonal Anti-IL8 antibody produced in mouse

description

clone 6217, purified immunoglobulin, lyophilized powder

description

clone 6G4, purified immunoglobulin, buffered aqueous solution

description

affinity isolated antibody, lyophilized powder

description

clone 4E3, purified immunoglobulin, buffered aqueous solution

biological source

mouse

biological source

mouse

biological source

goat

biological source

mouse

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

clone

6217, monoclonal

clone

6G4, monoclonal

clone

polyclonal

clone

4E3, monoclonal

Gene Information

human ... IL8(3576)

Gene Information

human ... IL8(3576)

Gene Information

human ... IL8(3576)

Gene Information

human ... IL8(3576)

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

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Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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