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I5283

Anti-INCENP antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-Inner Centromere Protein

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Size/SKUAvailabilityPrice
200 μL
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$666.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD05665235
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ARR, IF, WB CL
Citations:
8

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biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~106 kDa

species reactivity

human, mouse, rat

technique(s)

indirect immunofluorescence: 1:100 using HeLa cells, microarray: suitable, western blot (chemiluminescent): 1:2,000 using whole extract of mouse NIH3T3 and rat PC12 cells, western blot (chemiluminescent): 1:5,000 using nuclear extract of human HeLa cells

UniProt accession no.

Q9NQS7

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... INCENP(3619)
mouse ... Incenp(16319)
rat ... Incenp(293733)

General description

The gene INCENP (inner centromere protein) encodes an inner centromere protein that forms a component of the chromosomal passenger complex (CPC). It is found to be localized to inner centromeres during mitosis. The CPC also contains Aurora B, survivin, and Borealin.

Immunogen

synthetic peptide corresponding to amino acid residues 884-901 of human INCENP with N-terminal added cysteine conjugated to KLH. The corresponding sequence differs by one amino acid in chicken and Xenopus laevis and by two amino acids in mouse.

Application

Anti-INCENP antibody produced in rabbit has been used in western blotting and immunofluorescence.

Biochem/physiol Actions

Studies in knockout mice established INCENP (inner centromere protein) as essential for mouse development and viability. Loss of INCENP results in chromosome segregation defects and failures in cytokinesis.
The inner centromere protein encoded by the INCENP gene is phosphorylated by Cdk1 (cyclin dependent kinase 1) in mitosis. It is found to be associated with Plk1 (Polo-like kinase) in a polo-box–dependent manner. Plk1 is a serine/threonine kinase that functions in cell cycle, especially in mitosis. INCEP participates in the localization of Bub1 (Budding uninhibited by benzimidazole 1), a serine/threonine kinase involved in spindle checkpoint, to kinetochore. This, in turn, promotes the recruitment of Plk1 to kinetochores. Phosphorylated INCENP stimulates the activity of Aurora B kinase at centromeres and the central spindle that is important for chromosome segregation and cytokinesis, respectively.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-INCENP antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

Sigma-Aldrich

I5283

Anti-INCENP antibody produced in rabbit

Anti-phospho-Cofilin (pSer3) antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

Sigma-Aldrich

C8992

Anti-phospho-Cofilin (pSer3) antibody produced in rabbit

description

IgG fraction of antiserum, buffered aqueous solution

description

IgG fraction of antiserum, buffered aqueous solution

Quality Level

200

Quality Level

200

biological source

rabbit

biological source

rabbit

antibody form

IgG fraction of antiserum

antibody form

IgG fraction of antiserum

conjugate

unconjugated

conjugate

unconjugated

Gene Information

human ... INCENP(3619)
mouse ... Incenp(16319)
rat ... Incenp(293733)

Gene Information

human ... CFL1(1072)
mouse ... Cfl1(12631)
rat ... Cfl1(29271)

technique(s)

indirect immunofluorescence: 1:100 using HeLa cells, western blot (chemiluminescent): 1:2,000 using whole extract of mouse NIH3T3 and rat PC12 cells, microarray: suitable, western blot (chemiluminescent): 1:5,000 using nuclear extract of human HeLa cells

technique(s)

western blot: 1:2,000-1:4,000 using whole extracts of HeLa human epithelioid carcinoma and mouse NIH3T3 cells

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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