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Anti-Integrin β1 antibody, Mouse monoclonal

Name: Anti-Integrin β1 antibody, Mouse monoclonal
Brand: SIGMA
Price: 571 USD
Availability: InStock

clone W1B10, purified from hybridoma cell culture

Synonym(s):

Anti-CD29

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Size/SKU	Availability	Price
0.2 mL	Estimated to ship onAugust 17, 2026fromMILWAUKEE	$571.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD02178888

Conjugate:

unconjugated

Clone:

W1B10, monoclonal

Application:

IF, IP, WB

Citations:

$571.00

Estimated to ship onAugust 17, 2026Details

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture, purified immunoglobulin

antibody product type

primary antibodies

clone

W1B10, monoclonal

form

buffered aqueous solution

mol wt

antigen 100-140 kDa

contains

15 mM sodium azide

species reactivity

chicken

technique(s)

immunoprecipitation (IP): suitable, indirect immunofluorescence: suitable, western blot: 1:200 using denatured, non-reduced extract of cultured fibroblasts from chicken embryo lower leg

isotype

IgG1

UniProt accession no.

P07228

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

chicken ... ITGB1(374058)

Description

General description

Monoclonal anti-β-1-Chicken Integrin (mouse IgG1 isotype) is derived from the W1B10 hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified chicken integrin from fibroblasts of 11 days old chicken embryos. Chicken integrin is a heterodimer consisting of the common β-1 subunit and two α subunits (α-5 or α-3). The β-1 subunit of chicken integrin is a 803-amino acid transmembrane glycoprotein consisting of a large extracellular domain and a short cytoplasmic domain.

Immunogen

chicken integrin.¹

Application

Anti-Integrin β1 antibody, Mouse monoclonal has been used in western blot, fluorescent activated cell sorter (FACS) analysis.

Biochem/physiol Actions

Integrin binding to extracellular matrix (ECM) ligands regulates cell adhesion, shape, motility, intracellular signalling and gene expression. The monoclonal anti-b-1 chicken integrin antibody (clone W1B10) has been used to inhibit chicken muscle cell adhesion in cell cultures.

The antibody recognizes an epitope on the extracellular side of the avian integrin complex, and inhibits adhesion of embryo and tendon fibroblasts as well as skeletal myoblasts to both laminin and fibronectin. Under reducing and denaturing conditions using extracts of chicken embryo muscle cells, the antibody precipitates the β₁ class integrin heterodimer as a broad band of ~140 kDa. In the absence of reducing agents, the integrin β₁ subunit appears as bands at 100 kDa (immature form), 100 kDa (mature form), and 140 kDa (noncovalently associated α-subunits). The antibody may also be used for bioactivity studies in adhesion assays.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin with 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	A2859	M0691	SAB4200715
Sigma-Aldrich I8638 Anti-Integrin β1 antibody, Mouse monoclonal	Sigma-Aldrich A2859 Monoclonal Anti-ATG5 antibody produced in mouse	Sigma-Aldrich M0691 Anti-Myosin VI antibody, Mouse monoclonal	Sigma-Aldrich SAB4200715 Anti-β-tubulin antibody, Mouse monoclonal
description clone W1B10, purified from hybridoma cell culture	description clone ATG5-18, purified immunoglobulin, buffered aqueous solution	description ~1 mg/mL, clone MUD-19, purified from hybridoma cell culture	description clone 2-28-33, purified from hybridoma cell culture
antibody form purified from hybridoma cell culture, purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified from hybridoma cell culture	antibody form purified from hybridoma cell culture
biological source mouse	biological source mouse	biological source mouse	biological source mouse
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
species reactivity chicken	species reactivity human, mouse, rat	species reactivity human, mouse, rat	species reactivity dog, chicken, rat, bovine, monkey, human, mouse
Gene Information chicken ... ITGB1(374058)	Gene Information human ... ATG5(9474) mouse ... Atg5(11793) rat ... Atg5(365601)	Gene Information human ... MYO6(4646) mouse ... Myo6(17920) rat ... Myo6(315840)	Gene Information human ... TUBB(203068) mouse ... Tubb3(22152)
clone W1B10, monoclonal	clone ATG5-18, monoclonal	clone MUD-19, monoclonal	clone 2-28-33, monoclonal

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c