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Monoclonal Anti-Involucrin antibody produced in mouse

Name: Monoclonal Anti-Involucrin antibody produced in mouse
Brand: SIGMA
Price: 601 USD
Availability: InStock

clone SY5, ascites fluid

Synonym(s):

Anti-Involucrin

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Size/SKU	Availability	Price
100 μL	Check Cart for Availability	$601.00
0.2 mL	Check Cart for Availability	$753.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.43

MDL number:

MFCD00212983

Conjugate:

unconjugated

Clone:

SY5, monoclonal

Application:

ELISA (i), ICC, IF, IHC (f), IHC (p), IP, WB

Citations:

$601.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

SY5, monoclonal

contains

15 mM sodium azide

species reactivity

canine, gorilla, owl monkey, pig, human

technique(s)

immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunohistochemistry (frozen sections): suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, indirect immunofluorescence: 1:100 using formalin-fixed, paraffin-embedded human skin sections, western blot: suitable

isotype

IgG1

UniProt accession no.

P07476

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... IVL(3713)

Description

General description

Involucrin is a soluble precursor protein that regulates terminal differentiation in keratinocytes. This protein is initially present in the cytosol component of keratinocytes and later associates with membrane proteins. Involucrin is expressed in squamous cell cancer and its expression is down-regulated by HPV 16 oncoproteins . Monoclonal Anti-Involucrin is specific for involucrin in primate and non-primate mammals. In cultured human keratinocytes, the antibody stains a 120 kDa band by immunoblot under denaturing-reducing conditions. However, in other cells the antibody binds to involucrin protein in varied sizes: 170 kDa in human breast adenocarcinoma cell line MCF-7, a doublet of approx. 115 and 150 kDa in gorilla and owl monkey, 66 kDa in dog, and a doublet at approx 105 kDa in pig. The antibody does not bind to mouse epidermis; hence the antibody can be used to study human xenografts in nude mice. The antibody stains the upper spinous and granular layers in human skin and the cytoplasm of suprabasal terminally differentiated keratinocytes in stratified colonies .

Immunogen

human involucrin.

Application

Monoclonal Anti-Involucrin antibody is suitable for use in immunofluorescence at 1:100 dilutions using keratinocytes or for indirect immunofluorescence using methanol fixed mouse embryonic fibroblasts-keratinocytes co-cultures . The antibody may also be used for western blot (1:1000) and immunohistochemical analysis . Additionally, the product can be useful for indirect ELISA, immunoblotting, immunoprecipitation and immunocytochemistry.

Biochem/physiol Actions

The antibody detects involucrin in a range of primate and nonprimate mammals in a variety of techniques. In immunoblotting of cultured human keratinocytes, the antibody stains a 120 kDa band under denaturing and reducing conditions. However, in other systems, the antibody stains the involucrin protein in a variety of sizes: 170 kDa in human breast adenocarcinoma cell line MCF-7, a doublet of approximately 115 and 150 kDa in gorilla and owl monkey, 66 kDa in dog, and a doublet of ~105 kDa in pig. The antibody does not react with mouse epidermis, permitting use in studies of human xenografts in nude mice. The antibody stains the upper spinous and granular layers in human skin and the cytoplasm of suprabasal terminally differentiated keratinocytes in stratified colonies.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	SAB5702058	ZRB1399	HPA055211
Sigma-Aldrich I9018 Monoclonal Anti-Involucrin antibody produced in mouse	Sigma-Aldrich SAB5702058 Anti-Involucrin Antibody, clone 8H7B3, Rabbit Monoclonal	Sigma-Aldrich ZRB1399 Anti-Involucrin Antibody, clone 1D13 ZooMAb^® Rabbit Monoclonal	Sigma-Aldrich HPA055211 Anti-IVL antibody produced in rabbit
description clone SY5, ascites fluid	description -	description recombinant, expressed in HEK 293 cells	description Prestige Antibodies^® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
clone SY5, monoclonal	clone 8H7B3, monoclonal	clone 1D13, recombinant monoclonal	clone polyclonal
biological source mouse	biological source rabbit	biological source rabbit	biological source rabbit
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
antibody form ascites fluid	antibody form -	antibody form purified antibody	antibody form affinity isolated antibody
species reactivity canine, gorilla, owl monkey, pig, human	species reactivity human, rat	species reactivity human	species reactivity human
Gene Information human ... IVL(3713)	Gene Information human ... IVL(3713)	Gene Information -	Gene Information human ... IVL(3713)

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Storage Class

10 - Combustible liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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