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Anti-Jagged 1 antibody produced in goat

Name: Anti-Jagged 1 antibody produced in goat
Brand: SIGMA
Price: 651 USD
Availability: InStock

affinity isolated antibody, lyophilized powder

Synonym(s):

Anti-AGS, Anti-AGS1, Anti-AHD, Anti-AWS, Anti-CD339, Anti-CMT2HH, Anti-DCHE, Anti-HJ1, Anti-JAGL1

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Size/SKU	Availability	Price
0.1 mg	Available to ship TODAYfromMILWAUKEE	$628.00 $651.00

About This Item

UNSPSC Code:

51111800

NACRES:

NA.41

MDL number:

MFCD02096779

Conjugate:

unconjugated

Clone:

polyclonal

Application:

ELISA (c), FACS, WB

Citations:

$651.00

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Properties

biological source

goat

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

species reactivity

rat

technique(s)

capture ELISA: 0.2-0.8 μg/mL, flow cytometry: 2.5 μg using 10⁶rat cortical stem cells, western blot: 0.1 μg/mL

UniProt accession no.

Q63722

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... JAG1(182)
rat ... Jag1(29146)

Description

General description

Jagged-1 is a Notch ligand. The Notch signaling system regulates proliferation, differentiation, and apoptosis in many tissues, including hematopoietic tissue. Notch is a family of transmembrane receptors activated by ligands (Jagged-1, Jagged-2, and Delta-1) that are expressed on adjacent cells. Hematopoietic stem cells and early progenitors express Notch-1 and Notch-2, whereas primary stromal cultures have been shown to express Jagged-1, indicating that Jagged-1 may play a role in nurturing progenitor cells within the hematopoietic microenvironment. Cells engineered to express high levels of Jagged-1 caused a four-fold increase in formation of hematopoietic progenitor cell colonies. However, without the presence of other cytokines that stimulate proliferation and maturation, such as IL-3, IL-6, SCF and G-CSF, Jagged-1 actually decreased myeloid colony formation of CD34⁺ cells, suggesting that Jagged-1 modulates cytokine signals and helps preserve CD34⁺ cells in an immature state.

Notch signaling is a well conserved among the metazoans and is a regulator of growth, differentiation, and patterning processes. One of the ligands of Notch family receptors is Jagged. Mammalian Jagged family comprises of Jagged-1 and Jagged-2. Jagged proteins are expressed during the critical stages of embryonic and postnatal development. The Notch-Jagged signaling regulates the proliferation of endothelial cells, apoptosis, differentiation of hematopoietic progenitors, skeletal development and formation and differentiation of blood vessels. Notch-jagged pathway components play important role in the pathology of diseases like Alagille syndrome, CADASIL (cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy) and osteosarcoma. Anti-Jagged 1 antibody specifically recognizes rat Jagged-1.

Immunogen

recombinant rat Jagged 1 extracellular domain, expressed in NSO cells.

Application

Anti-Jagged 1 antibody may be used for ELISA at a working antibody concentration of 0.5-1.0 μg/ml. For immunoblotting, antibody concentration of 0.1-0.2 μg/ml is recommended. For flow cytometry, a concentration of 20 μg/ml (10 μL/10⁵ cells to 10⁶ cells) may be used. The antibody is suitable for neutralization assays (ND50 is ~2-10 μg/ml).

Physical form

lyophilized from a 0.2 μm filtered solution of phosphate buffered saline (PBS), pH 7.4, and 5% trehalose

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	ZRB1724	MABS2287	SAB1405437
Sigma-Aldrich J4127 Anti-Jagged 1 antibody produced in goat	Sigma-Aldrich ZRB1724 Anti-Jagged1 Antibody, clone 1C23 ZooMAb^® Rabbit Monoclonal	MABS2287 Anti-Jagged2 Antibody, clone HMJ2-1	Sigma-Aldrich SAB1405437 Anti-JAG1 antibody produced in mouse
description affinity isolated antibody, lyophilized powder	description recombinant, expressed in HEK 293 cells	description -	description purified immunoglobulin, buffered aqueous solution
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
biological source goat	biological source rabbit	biological source hamster	biological source mouse
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 100
antibody form affinity isolated antibody	antibody form purified antibody	antibody form purified antibody	antibody form purified immunoglobulin
storage temp. −20°C	storage temp. 2-8°C	storage temp. -10 to -25°C	storage temp. −20°C
Gene Information human ... JAG1(182) rat ... Jag1(29146)	Gene Information human ... JAG1(182)	Gene Information mouse ... Jag2(16450)	Gene Information human ... JAG1(182)

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Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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