Skip to Content
MilliporeSigma

Key Documents

View All Documentation

Skip To

K0383

Maleimide Activated KLH

Synonym(s):

Maleimide Activated Keyhole Limpet Hemocyanin

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Size/SKUAvailabilityPrice
5 mg
Check Cart for Availability
$287.00

About This Item

NACRES:
NA.32
UNSPSC Code:
12352111
eCl@ss:
32150917
Form:
lyophilized powder
Biological source:
Megathura crenulata

$287.00

Check Cart for Availability
​
Request a Custom Order
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

Megathura crenulata

Quality Segment

form

lyophilized powder

technique(s)

bioconjugation: suitable

storage temp.

2-8°C

General description

Maleimide Activated KLH is provided in lyophilized, ready to-use form, eliminating the need for time-consuming activation and purification steps of the carrier protein. Maleimide Activated KLH contains approximately 160-320 maleimide groups for each KLH molecule. The conjugation reaction with a cysteine containing peptide is performed at pH 6.6-7.0. The extent of conjugation can then be determined by a colorimetric assay with Ellman′s reagent to detect unreacted thiol groups in the cysteine-containing peptide.

Application

Maleimide Activated KLH has been used in peptide conjugation.
Maleimide-activated KLH (mKLH) has reactive maleimide groups on its surface, available for conjugation with a cysteine-containing peptide or a thiol-containing hapten. The reaction of the maleimide group and the thiol group proceeds rapidly and selectively under mild coupling conditions (pH 6.5-7.5) to yield a stable, covalently linked peptide-protein conjugate that may be used in immunization protocols or in antibody screening methods.

Physical form

Each vial contains 5 mg of Maleimide Activated KLH lyophilized from 10 mM sodium phosphate buffer, pH 6.6, with 0.115 mM NaCl, 1 mM EDTA, and 40 mM sucrose as stabilizer.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
H7017374805374813
Maleimide Activated KLH

Sigma-Aldrich

K0383

Maleimide Activated KLH

Hemocyanin from Megathura crenulata (keyhole limpet)

Sigma-Aldrich

H7017

Hemocyanin from Megathura crenulata (keyhole limpet)

Hemocyanin, Keyhole Limpet, Megathura crenulata Hemocyanin, Keyhole Limpet, Megathura crenulata, CAS 9013-72-3, is a large, multi-subunit, oxygen-carrying, metalloprotein that is used as a carrier protein in the production of antibodies.

Sigma-Aldrich

374805

Hemocyanin, Keyhole Limpet, Megathura crenulata

Hemocyanin, Keyhole Limpet, Megathura crenulata, High Purity, Endotoxin-Free, Glycerol Hemocyanin, Keyhole Limpet, Megathura crenulata, High Purity, Endotoxin Free, is a large, multi-subunit, O2-carrying, metalloprotein that is used as a carrier protein in the production of antibodies.

Sigma-Aldrich

374813

Hemocyanin, Keyhole Limpet, Megathura crenulata, High Purity, Endotoxin-Free, Glycerol

description

-

description

-

description

Hemocyanin, Keyhole Limpet, Megathura crenulata, CAS 9013-72-3, is a large, multi-subunit, oxygen-carrying, metalloprotein that is used as a carrier protein in the production of antibodies.

description

Hemocyanin, Keyhole Limpet, Megathura crenulata, High Purity, Endotoxin Free, is a large, multi-subunit, O2-carrying, metalloprotein that is used as a carrier protein in the production of antibodies.

technique(s)

bioconjugation: suitable

technique(s)

ELISA: suitable

technique(s)

-

technique(s)

-

biological source

Megathura crenulata

biological source

Megathura crenulata

biological source

-

biological source

-

form

lyophilized powder

form

powder

form

lyophilized solid

form

liquid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

100

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Questions

Reviews

No rating value

Active Filters