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Anti-Lamin A (C-terminal) antibody produced in rabbit

Name: Anti-Lamin A (C-terminal) antibody produced in rabbit
Brand: SIGMA
Price: 614 USD
Availability: InStock

Enhanced Validation

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-LAMA, Anti-LMN1, Anti-LMNA, Anti-Lamin A/C

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Size/SKU	Availability	Price
200 μL	Check Cart for Availability	$614.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD01633588

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IF, WB

Citations:

$614.00

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~70 kDa

species reactivity

rat, human, mouse

enhanced validation

independent
Learn more about Antibody Enhanced Validation

concentration

~1 mg/mL

technique(s)

indirect immunofluorescence: 1-2 μg/mL using human HeLa, rat NRK, and mouse 3T3 cells, western blot: 0.1-0.2 μg/mL using human HeLa nuclear extract

UniProt accession no.

P02545

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... LMNA(4000)
mouse ... Lmna(16905)
rat ... Lmna(60374)

Description

General description

Lamin is a structural protein of the nuclear lamina. Lamin A is a type A lamin encoded by the LMNA gene. Lamin A contains 664 amino acids and is expressed in most somatic cells. It contains α-helical rod domain to enable assembly into filaments, a nuclear localization sequence, and a carboxy-terminal CAAX box isoprenylation sequence for nuclear membrane targeting.

Immunogen

synthetic peptide corresponding to amino acids 598-611 of human lamin A with a C-terminal added cysteine, conjugated to KLH. The corresponding sequence differs by one amino acid in rat, three amino acids in mouse, and by a gap of one amino acid in both rat and mouse lamin A.

Application

Anti-Lamin A (C-terminal) antibody produced in rabbit has been used in:

western blotting
immunofluorescence microscopy
immunohistochemistry

Anti-Lamin A (C-terminal) antibody is suitable for use in western blot (0.1-0.2 μg/mL) using HeLa nuclear extract. This antibody can also be used in indirect immunofluorescence (1-2 μg/mL) using HeLa cells, rat NRK and mouse 3T3 cells. Additionally, anti-Lamin A (C-terminal) antibodies are suitable for use in immunoblotting (approx. 70 kDa). Cleaved fragments of lamin A may form additional bands at 45-50 kDa.

Biochem/physiol Actions

Lamin A cut into a 47kDa fragment that facilitates chromatin condensation and nuclear degradation during cell death

Mutations in lamin A and C have been linked to a variety of rare human diseases including muscular dystrophy, lipodystrophy, cardiomyopathy, neuropathy, and progeroid syndromes (collectively termed laminopathies) and to premature aging (Hutchinson-Gilford progeria syndrome).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	SAB4200236	SAB4501764	SAB5700618
Sigma-Aldrich L1293 Anti-Lamin A (C-terminal) antibody produced in rabbit	Sigma-Aldrich SAB4200236 Monoclonal Anti-Lamin A/C antibody produced in mouse	Sigma-Aldrich SAB4501764 Anti-Lamin A antibody produced in rabbit	Sigma-Aldrich SAB5700618 Anti-Lamin A/C antibody produced in rabbit
description ~1 mg/mL, affinity isolated antibody, buffered aqueous solution	description clone 4C11, purified from hybridoma cell culture	description affinity isolated antibody	description -
Quality Level 200	Quality Level 200	Quality Level 100	Quality Level 100
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
antibody form affinity isolated antibody	antibody form purified immunoglobulin	antibody form affinity isolated antibody	antibody form affinity isolated antibody
biological source rabbit	biological source mouse	biological source rabbit	biological source rabbit
shipped in dry ice	shipped in dry ice	shipped in wet ice	shipped in wet ice
technique(s) indirect immunofluorescence: 1-2 μg/mL using human HeLa, rat NRK, and mouse 3T3 cells, western blot: 0.1-0.2 μg/mL using human HeLa nuclear extract	technique(s) immunoprecipitation (IP): suitable, indirect immunofluorescence: suitable, western blot: 0.1-0.2 μg/mL using whole extracts of human HeLa cells	technique(s) ELISA: 1:1000, immunofluorescence: 1:100-1:500, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000	technique(s) immunofluorescence: 1:50-1:200, immunohistochemistry: 1:50-1:200, immunoprecipitation (IP): 1:50-1:200, western blot: 1:500-1:2000

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

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