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L-Lactic Dehydrogenase from bovine muscle

Name: <SC>L</SC>-Lactic Dehydrogenase from bovine muscle
Brand: SIGMA
Price: 374 USD
Availability: InStock

Type X, ammonium sulfate suspension, ≥600 units/mg protein

Synonym(s):

(S)-Lactate: NAD⁺ oxidoreductase, L-LDH, LAD, LD, Lactate

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Size/SKU	Availability	Price
3000 units	Check Cart for Availability	$374.00
15000 units	Check Cart for Availability	$1,700.00

About This Item

CAS Number:

9001-60-9

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-617-8

MDL number:

MFCD00131466

EC Number:

1.1.1.27 (BRENDA, IUBMB)

Specific activity:

≥600 units/mg protein

Assay:

3.0—25.0 mg protein/mL (biuret)

Biological source:

bovine muscle

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Properties

biological source

bovine muscle

Quality Segment

200

type

Type X

assay

3.0—25.0 mg protein/mL (biuret)

form

ammonium sulfate suspension

specific activity

≥600 units/mg protein

manufacturer/tradename

Sigma-Aldrich

technique(s)

activity assay: suitable

color

white

pH

7.5

UniProt accession no.

P19858, Q5E9B1

application(s)

life science and biopharma

foreign activity

pyruvate kinase ≤0.01%

storage temp.

2-8°C

Gene Information

cow ... LDHA(281274), LDHB(281275)

Description

General description

Research Area: Cell Signaling
Lactate dehydrogenase (LDH) is a key enzyme in the anaerobic metabolic pathway and falls under the class of oxidoreductases. This enzyme is found in a wide range of organisms, including both plants and animals. It is widely distributed across all tissues. LDH is primarily a cytoplasmic enzyme, with particularly high concentrations in muscle, liver, and kidney tissues.

Application

L-Lactic Dehydrogenase from bovine muscle has been used in lactate assay to assess lactate tissue accumulation.

Biochem/physiol Actions

Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

Lactate dehydrogenase (LDH) plays a critical role as a checkpoint in gluconeogenesis and DNA metabolism. It plays a vital role in maintaining homeostasis during periods of low oxygen availability. As an oxidoreductase enzyme, lactate dehydrogenase catalyzes the reversible conversion of pyruvate to lactate using NADH. This enzyme is crucial for the anaerobic metabolism of glucose when oxygen is either absent or in short supply. LDH is the sole serum biomarker effective for evaluating metastatic melanomas.

Physical form

Crystalline suspension in 2.4 M (NH₄)₂SO₄ solution, pH 6.0

Analysis Note

Protein determined by biuret.

Other Notes

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

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This Item	L2625	L7525	L2500
Sigma-Aldrich L1378 L-Lactic Dehydrogenase from bovine muscle	Sigma-Aldrich L2625 L-Lactic Dehydrogenase from bovine heart	Sigma-Aldrich L7525 L-Lactic Dehydrogenase from porcine heart	Sigma-Aldrich L2500 L-Lactic Dehydrogenase from rabbit muscle
description Type X, ammonium sulfate suspension, ≥600 units/mg protein	description Type III, ammonium sulfate suspension, ≥500 units/mg protein	description ammonium sulfate suspension, ≥200 units/mg protein	description Type II, ammonium sulfate suspension, 800-1,200 units/mg protein
technique(s) activity assay: suitable	technique(s) -	technique(s) -	technique(s) -
Gene Information cow ... LDHA(281274), LDHB(281275)	Gene Information cow ... LDHA(281274), LDHB(281275)	Gene Information pig ... LDHA(407245)	Gene Information -
assay 3.0—25.0 mg protein/mL (biuret)	assay -	assay -	assay -
biological source bovine muscle	biological source -	biological source Porcine heart	biological source -
specific activity ≥600 units/mg protein	specific activity ≥500 units/mg protein	specific activity ≥200 units/mg protein	specific activity 800-1,200 units/mg protein
form ammonium sulfate suspension	form ammonium sulfate suspension	form ammonium sulfate suspension	form ammonium sulfate suspension

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

ppe

Eyeshields, Gloves, type N95 (US)

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